TATA ADA MARIA


Responsabile dell'U.O.

Cognome e Nome

Tata Ada Maria

Qualifica

PO

Dipartimento

Biologia e Biotecnologie Charles Darwin

Settore scientifico disciplinare

BIO/06

E-mail

adamaria.tata@uniroma1.it

Telefono

Personale strutturato

Cognome e Nome

Cacci Emanuele

Qualifica

Ricercatore

Dipartimento

Biologia e Biotecnologie Charles Darwin

Ente di appartenenza

Università di Roma “La Sapienza”

Cognome e Nome

Lupo Giuseppe

Qualifica

PA

Dipartimento

Biologia e Biotecnologie Charles Darwin

Ente di appartenenza

Università di Roma “La Sapienza”

Cognome e Nome

M.Elena Miranda Banos

Qualifica

PA

Dipartimento

Biologia e Biotecnologie Charles Darwin

Ente di appartenenza

Università di Roma “La Sapienza”

Cognome e Nome

Antonella De Jaco

Qualifica

PA

Dipartimento

Biologia e Biotecnologie Charles Darwin

Ente di appartenenza

Università di Roma “La Sapienza”

Cognome e Nome

Marco Fidaleo

Qualifica

PA

Dipartimento

Biologia e Biotecnologie Charles Darwin

Ente di appartenenza

Università di Roma “La Sapienza”

Personale non strutturato

Linee di ricerca

1.CHOLINERGIC CONTROL OF THE CELL GROWTH AND DIFFERENTIATION OF NORMAL AND PATHOLOGICAL GLIAL CELLS. Cholinergic control of myelinating glial cell proliferation and differentiation isinvestigated. Schwann cell cultures from neonatal rat or Schwann–induced from rat and human mesenchymal stem cells are used as experimental model. The ability of muscarinic agonists to modulate the growth and survival of glioblastoma cancer stem cells obtained from human biopsies is also investigated. The possible altered functions of muscarinic and nicotinic cholinergic receptors in the control of inflammatory states in demyelinating disorders (multiple sclerosis) are also considered. 2. ACTIVATION OF NEURAL DIFFERENTIATION PROGRAMS: TRANSCRIPTION FACTORS AND ENVIRONMENTAL SIGNALS. The aim of the project is to investigate the ability of transcription factors, whose expression is activated during neural differentiation, to direct undifferentiated cells to specific differentiation patterns and to test the responsiveness of cells, representing successive steps of differentiation, to different environments. Isolation and characterization of stem cells have become a major issue in neurobiology and the demonstration of stem cells in the adult brain has opened a new field in this area. The following points are under investigation: 1. EGR-1 and REST role in stem cell differentiation 2. Activation of neurospecific gene expression via neurotransmitter receptor activation. 3.Epigenetic modification associated to NPs differentiation and maintenance of regional identity. 3. ANALYSIS OF GENE MUTATION ASSOCIATED TO NEURODEVELOPMENT DISORDERS. The project is focused on the functional analysis of mutated proteins using in vitro and in vivo models in order to clarify the mechanisms responsible of the neurological disorders (i.e. Autism and Fenib) and identify possible new therapeutic targets.

Tecnologie in uso dall'UO

  1. 1.
    Culture of murine and human cell lines and primary cultures
  2. 2.
    Isolation and culture of NPS
  3. 3.
    Cell Transfection
  4. 4.
    PCR and Real Time PCR
  5. 5.
    Western and immunoblot
  6. 6.
    In situ hybridization
  7. 7.
    Immunocyto-histochemistry

Strumentazione

Denominazione

Real Time PCR (Bio-Rad)
P2 lab
Electron Microscope
Confocal Microscope (Leica)
Microscope with Apotome system (Zeiss) (Fluorescence and time lapse)
Reader Plate Glomax Multi Detection System
Cryostat and ultramicrotome

Struttura ove la strumentazione è allocata

Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin

Responsabile

Prof.ssa A. Tata
Prof.ssa A. Tata
Prof.ssa E. De Stefano
Prof.ssa Sabatini
Prof. M. Fidaleo
Dr.ssa De Jaco
Prof.ssa E. De Stefano

Pubblicazioni

Dottorati di ricerca

Componente UO

Dottorato di ricerca

Coordinatore

Sede