Responsabile dell'U.O.
Cognome e Nome
Tata Ada Maria
Qualifica
PO
Dipartimento
Biologia e Biotecnologie Charles Darwin
Settore scientifico disciplinare
BIO/06
adamaria.tata@uniroma1.it
Telefono
Personale strutturato
Cognome e Nome
Cacci Emanuele
Qualifica
Ricercatore
Dipartimento
Biologia e Biotecnologie Charles Darwin
Ente di appartenenza
Università di Roma “La Sapienza”
Cognome e Nome
Lupo Giuseppe
Qualifica
PA
Dipartimento
Biologia e Biotecnologie Charles Darwin
Ente di appartenenza
Università di Roma “La Sapienza”
Cognome e Nome
M.Elena Miranda Banos
Qualifica
PA
Dipartimento
Biologia e Biotecnologie Charles Darwin
Ente di appartenenza
Università di Roma “La Sapienza”
Cognome e Nome
Antonella De Jaco
Qualifica
PA
Dipartimento
Biologia e Biotecnologie Charles Darwin
Ente di appartenenza
Università di Roma “La Sapienza”
Cognome e Nome
Marco Fidaleo
Qualifica
PA
Dipartimento
Biologia e Biotecnologie Charles Darwin
Ente di appartenenza
Università di Roma “La Sapienza”
Personale non strutturato
Linee di ricerca
1.CHOLINERGIC CONTROL OF THE CELL GROWTH AND DIFFERENTIATION OF NORMAL AND PATHOLOGICAL GLIAL CELLS.
Cholinergic control of myelinating glial cell proliferation and differentiation isinvestigated. Schwann cell cultures from neonatal rat or Schwann–induced from rat and human mesenchymal stem cells are used as experimental model. The ability of muscarinic agonists to modulate the growth and survival of glioblastoma cancer stem cells obtained from human biopsies is also investigated. The possible altered functions of muscarinic and nicotinic cholinergic receptors in the control of inflammatory states in demyelinating disorders (multiple sclerosis) are also considered.
2. ACTIVATION OF NEURAL DIFFERENTIATION PROGRAMS: TRANSCRIPTION FACTORS AND ENVIRONMENTAL SIGNALS.
The aim of the project is to investigate the ability of transcription factors, whose expression is activated during neural differentiation, to direct undifferentiated cells to specific differentiation patterns and to test the responsiveness of cells, representing successive steps of differentiation, to different environments. Isolation and characterization of stem cells have become a major issue in neurobiology and the demonstration of stem cells in the adult brain has opened a new field in this area. The following points are under investigation: 1. EGR-1 and REST role in stem cell differentiation 2. Activation of neurospecific gene expression via neurotransmitter receptor activation. 3.Epigenetic modification associated to NPs differentiation and maintenance of regional identity.
3. ANALYSIS OF GENE MUTATION ASSOCIATED TO NEURODEVELOPMENT DISORDERS.
The project is focused on the functional analysis of mutated proteins using in vitro and in vivo models in order to clarify the mechanisms responsible of the neurological disorders (i.e. Autism and Fenib) and identify possible new therapeutic targets.
Tecnologie in uso dall'UO
- Culture of murine and human cell lines and primary cultures
- Isolation and culture of NPS
- Cell Transfection
- PCR and Real Time PCR
- Western and immunoblot
- In situ hybridization
- Immunocyto-histochemistry
Strumentazione
Denominazione
Real Time PCR (Bio-Rad)
P2 lab
Electron Microscope
Confocal Microscope (Leica)
Microscope with Apotome system (Zeiss) (Fluorescence and time lapse)
Reader Plate Glomax Multi Detection System
Cryostat and ultramicrotome
Struttura ove la strumentazione è allocata
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Biologia e Biotecnologie Charles Darwin
Responsabile
Prof.ssa A. Tata
Prof.ssa A. Tata
Prof.ssa E. De Stefano
Prof.ssa Sabatini
Prof. M. Fidaleo
Dr.ssa De Jaco
Prof.ssa E. De Stefano