SPOLAORE BARBARA

Responsabile dell'U.O.

Cognome e Nome

SPOLAORE Barbara

Qualifica

PA

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Settore scientifico disciplinare

BIO-10

E-mail

barbara.spolaore@unipd.it

Telefono

049-8275702

Personale strutturato

Cognome e Nome

DE FILIPPIS Vincenzo

Qualifica

PA

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Cognome e Nome

POLVERINO DE LAURETO Patrizia

Qualifica

Professore emerito

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Cognome e Nome

ACQUASALIENTE Laura

Qualifica

RTDB

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Cognome e Nome

PIERANGELINI ANDREA

Qualifica

Technician

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Personale non strutturato

Cognome e Nome

DEI ROSSI Alessia

Qualifica

Ph.D student

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Cognome e Nome

CAVEDON Elena

Qualifica

Ph.D student

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Cognome e Nome

FONGARO Benedetta

Qualifica

Post-doc

Dipartimento

Pharmaceutical and Pharmacological Sciences (DSF)

Ente di appartenenza

University of Padova

Linee di ricerca

The research concerns protein structure, protein folding and misfolding by using biochemical and biophysical technologies. These methodologies are applied on different protein systems of physio-pathological relevance, and in particular involved in cardiovascular diseases and amyloidosis. The U.O. has also experience in the production, characterisation and conjugation of proteins and peptides for biotechnological and pharmaceutical applications.

Tecnologie in uso dall'UO

  1. 1.
    Protein production by recombinant technologies
  2. 2.
    Protein purification by HPLC and FPLC chromatographic techniques
  3. 3.
    Bio-spectroscopy (UV-Visible absorption, fluorescence and circular dichroism spectroscopies)
  4. 4.
    Mass spectrometry (MS) for the chemical characterization of biomolecules
  5. 5.
    Hydrogen-Deuterium Exchange MS (HDX-MS)
  6. 6.
    Surface Plasmon Resonance
  7. 7.
    Dynamic light Scattering
  8. 8.
    Peptide Synthesis
  9. 9.
    Isothermal Titration Calorimetry applied to molecular recognition
  10. 10.
    Blood coagulation assays

Strumentazione

Denominazione

ESI/Q-TOF Xevo G2S (Waters) connected to an UPLC Acquity M-class (Waters)
Automation 2.0 sample workstation (Waters) connected to a HDX PAL-2 autosampler (Leap Technologies)
ESI/Q-TOF mod. 6550 (Agilent) connected to an UPLC mod. 1290 (Agilent)
Bio-spectroscopy: Circular dichroism (Jasco, J-815), Fluorescence (Jasco, FP-6500) SFM-3000 stopped-flow system (BioLogic).
Surface Plasmon Resonance (BiaCore X-100)
Isothermal Titration Calorimetry (Malvern, VP-ITC)
Differential Scanning Calorimetry (Malvern, VP-DSC)
Dynamic Light Scattering (DLS) (Malvern, Ultra-ZS)
Automated Peptide Synthesizer mod. PS3 (Gyros Protein Technologies)
FPLC (Akta Purifier) and HPLC systems (Jasco, PU-1580, PU-2089, PU4180; Agilent, 1100, 1200)

Struttura ove la strumentazione è allocata

DSF
DSF
DSF
DSF
DSF
DSF
DSF
DSF
DSF
DSF

Responsabile

Department Facility
Department Facility
Department Facility
Department Facility
Department Facility
Department Facility
Department Facility
Department Facility
De Filippis Vincenzo
De Filippis Vincenzo, Patrizia Polverino de Laureto, Barbara Spolaore

Pubblicazioni

  1. 1.
    Acquasaliente L, Pierangelini A, Pagotto A, Pozzi N, De Filippis V. From haemadin to haemanorm: Synthesis and characterization of full-length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm). Protein Sci. 2023; 32(12):e4825. doi: 10.1002/pro.4825.
  2. 2.
    Fongaro B, Campara B, Moscatiello GY, De Luigi A, Panzeri D, Sironi L, Bigini P, Carretta G, Miolo G, Pasut G, Polverino De Laureto P. Assessing the physicochemical stability and intracellular trafficking of mRNA-based COVID-19 vaccines. Int J Pharm. 2023; 644:123319. doi: 10.1016/j.ijpharm.2023.123319.
  3. 3.
    Acquasaliente L, Pontarollo G, Radu CM, Peterle D, Artusi I, Pagotto A, Uliana F, Negro A, Simioni P, De Filippis V. Exogenous human α-Synuclein acts in vitro as a mild platelet antiaggregant inhibiting α-thrombin-induced platelet activation. Sci Rep. 2022; 12(1):9880. doi: 10.1038/s41598-022-12886-y.
  4. 4.
    Fongaro B, Cappelletto E, Sosic A, Spolaore B, Polverino de Laureto P. 3,4-Dihydroxyphenylethanol and 3,4-dihydroxyphenylacetic acid affect the aggregation process of E46K variant of α-synuclein at different extent: Insights into the interplay between protein dynamics and catechol effect. Protein Sci. 2022; 31(7):e4356. doi: 10.1002/pro.4356
  5. 5.
    Ceschi S, Berselli M, Cozzaglio M, Giantin M, Toppo S, Spolaore B, Sissi C. Vimentin binds to G-quadruplex repeats found at telomeres and gene promoters. Nucleic Acids Res. 2022; 50(3):1370-1381. doi: 10.1093/nar/gkab1274.
  6. 6.
    Bolzati C, Salvarese N, Spolaore B, Vittadini A, Forrer D, Brunello S, Ghiani S, Maiocchi A. Water-Soluble [Tc(N)(PNP)] Moiety for Room-Temperature 99mTc Labeling of Sensitive Target Vectors. Mol Pharm. 2022; 19(3):876-894. doi: 10.1021/acs.molpharmaceut.1c00816.
  7. 7.
    Palazzi L, Fongaro B, Leri M, Acquasaliente L, Stefani M, Bucciantini M, Polverino de Laureto P. Structural Features and Toxicity of α-Synuclein Oligomers Grown in the Presence of DOPAC. Int J Mol Sci. 2021; 22(11):6008. doi: 10.3390/ijms22116008.
  8. 8.
    Uliana F, Vizovišek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, Auf dem Keller U, Aebersold R. Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screen. Nat Commun. 2021;12(1):1693. doi: 10.1038/s41467-021-21754-8.
  9. 9.
    Scapin G, Gasparotto M, Peterle D, Tescari S, Porcellato E, Piovesan A, Righetto I, Acquasaliente* L, De Filippis* V, Filippini* F. A conserved Neurite Outgrowth and Guidance motif with biomimetic potential in neuronal Cell Adhesion Molecules. Comput Struct Biotechnol J. 2021; 19:5622-5636. doi:10.1016/j.csbj.2021.10.005. *Corresponding authors
  10. 10.
    Peterle D, Pontarollo G, Spada S, Brun P, Palazzi L, Sokolov AV, Spolaore B, Polverino de Laureto P, Vasilyev VB, Castagliuolo I, De Filippis V. A serine protease secreted from Bacillus subtilis cleaves human plasma transthyretin to generate an amyloidogenic fragment. Commun Biol. 2020; 3(1):764. doi: 10.1038/s42003-020-01493-0.

Dottorati di ricerca

Componente UO

De Filippis Vincenzo
Polverino de Laureto Patrizia

Dottorato di ricerca

Scienze Molecolari
Medicina Biomolecolare

Coordinatore

Corni Stefano
Lucia De Franceschi

Sede

Università di Padova
Università di Padova