Saggio Isabella


Responsabile dell'U.O.

Cognome e Nome

Saggio Isabella

Qualifica

PA

Dipartimento

Biologia e biotecnologie Charles Darwin

Settore scientifico disciplinare

BIO/18

E-mail

isabella.saggio@uniroma1.it

Telefono

06.49912870

Personale strutturato

Cognome e Nome

FIAMMETTA VERNI

Qualifica

Ricercatore

Dipartimento

Biologia e biotecnologie Charles Darwin

Ente di appartenenza

Sapienza università di Roma

Cognome e Nome

ROMINA BURLA

Qualifica

Ricercatore

Dipartimento

Biologia e biotecnologie Charles Darwin

Ente di appartenenza

Sapienza università di Roma

Personale non strutturato

Linee di ricerca

Saggio-lab projects -Gene therapy vectors In the years we have been dealing with different viruses, including AAV, lentivirus, adenovirus and humanized phages. We use viral vectors for gene correction and disease modeling. Along with this we have been studying the properties of these viral vectors to understand their biology and possibly to design their amelioration. -Telomeres and lamins in natural and pathological aging We have characterized a new telomeric gene interacting with lamins named AKTIP. AKTIP properties put it at the crossroad of central aspects of human metabolism including DNA replication, nuclear architecture, telomeropathies, laminopathies and cancer. We’re studying the implication of this gene in the different metabolic pathways by in tube and in vivo analyses. Collaborative projects -Stem cell related bone disease We collaborate with P. Bianco lab (Sapienza Univeristy) in a project aiming at modeling, studying and correcting the bone genetic disease fibrous dysplasia. -Neonatal diabetes We collaborate with F. Barbetti lab (TorVergata University) for the development of a therapeutic approach for neonatal diabetes associated with dominant insulin gene mutations by means of allele specific silencing.

Tecnologie in uso dall'UO

  1. 1.
    Lentiviral vector construction for shRNA expression
  2. 2.
    Adenoviral vector production
  3. 3.
    Lambda and M13 phage technology (peptide libraries)
  4. 4.
    Lentiviral vectors for in vivo studies, in vivo injection and transgenic production
  5. 5.
    Mouse manipulation

Strumentazione

Denominazione

Real Time PCR machine
P2 cell culture facility
Confocal microscopy
FACS and Cell sorter
Animal house

Struttura ove la strumentazione è allocata

Dipartimento Genetica e Biologia Molecolare
Fondazione Scientifica Parco Biomedico di Roma
Dipartimento Genetica e Biologia Molecolare
Fondazione Scientifica Parco Biomedico di Roma
Fondazione Scientifica Parco Biomedico di Roma

Responsabile

R. Burla
R. Burla
R. Burla
R. Burla
Plaisant-M. La Torre

Pubblicazioni

Dottorati di ricerca

Componente UO

Dottorato di ricerca

Coordinatore

Sede