Pesce Vito


Responsabile dell'U.O.

Cognome e Nome

Pesce Vito

Qualifica

PA

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Settore scientifico disciplinare

BIO/10

E-mail

vito.pesce@uniba.it

Telefono

Personale strutturato

Cognome e Nome

Lezza M.S. Angela

Qualifica

PA

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Ente di appartenenza

University of Bari "Aldo Moro"

Cognome e Nome

Cormio Antonella

Qualifica

PA

Dipartimento

PRECISION AND REGENERATIVE MEDICINE AND IONIAN AREA

Ente di appartenenza

University of Bari "Aldo Moro"

Cognome e Nome

Loguercio Polosa Paola

Qualifica

PA

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Ente di appartenenza

University of Bari "Aldo Moro"

Cognome e Nome

BRUNI FRANCESCO

Qualifica

PA

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Ente di appartenenza

University of Bari "Aldo Moro"

Personale non strutturato

Cognome e Nome

DI LORENZO ROSA

Qualifica

Ph.D student

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Ente di appartenenza

University of Bari "Aldo Moro"

Cognome e Nome

RIZZO FRANCESCA

Qualifica

Ph.D student

Dipartimento

BIOSCIENCES, BIOTECHNOLOGY AND ENVIRONMENT

Ente di appartenenza

University of Bari "Aldo Moro"

Linee di ricerca

Study of the role of mitochondria and the mitochondrial genetic system in the aging process and in age-related pathological conditions (sarcopenia, diabetes, neurodegenerative diseases, cancer etc) as well as of the nutritional strategies or drugs that may prevent the degenerative processes, eventually promoting mitochondrial biogenesis and dynamics. Study of nuclear factors involved in the signaling pathways of mitochondrial biogenesis (PGC1s, Akt, SIRTs) and dynamics. Determination and quantification of mtDNA damages, as well as analysis of mtDNA stability and TFAM-mtDNA interactions. Experimental models are: i) tissue samples from rats and humans of different ages (collaboration with Prof. E. Marzetti, Univ. Cattolica - Sacro Cuore, Roma), ii) rats treated with caloric restriction diet (collaboration with Prof. C. Leeuwenburgh, Univ. of Florida, Gainesville, FL, USA and Prof. A. Picca, Department of Medicine and Surgery, LUM University, Casamassima, Bari), compounds qualified as nutraceuticals or drugs iii) cancer tissues samples. Studies on pathogenetic mechanisms responsible for the Leber Hereditary Optic Neuropathy. Role of mitochondrial biogenesis in modulating the disease penetrance and as predictive tool for the clinical manifestation of the disease. Studies on mitochondrial tRNA related diseases (MELAS and MERRF): evaluation of tissue-specific effects and rescuing mechanisms of therapeutic molecules derived from mitochondrial leucyl tRNA synthetase (collaboration with prof. G. d’Amati, Università La Sapienza, Roma). Study of mitochondrial DNA replication and transcription machinery in animal systems, aimed at the identification and characterization of new regulatory factors/enzymes (collaboration with prof. N.G. Larsson, Dept of Med. Biochem. and Biophys., Karolinska Institutet, Stockholm, Sweden). Different publications identifies Mgme1 exonuclease as a crucial regulator of mitochondrial DNA replication, as shown by mitochondrial pathologies caused by mutations in this gene.

Tecnologie in uso dall'UO

  1. 1.
    Cloning expression and purification of proteins in different eukaryotic and prokaryotic systems
  2. 2.
    Analysis of protein-protein and protein-DNA interactions: Chromatin Immunoprecipitation (nuclear and mitochondrial), in vivo proximity labelling
  3. 3.
    Spectrophotometric analysis of enzymatic activities
  4. 4.
    Sequence analysis. DNA mutations screening
  5. 5.
    Isolation characterization and quantification of DNA and RNA (PCR, RT-PCR, real-time PCR
  6. 6.
    Cell culture (mammalian cells and insect cells); creation and/or maintenance of stably and transiently transfected cell lines
  7. 7.
    Histochemical analysis of tissues micro-cryosections and Laser capture of single cells followed by Real Time PCR
  8. 8.
    Two-dimensional gel electrophoresis. Blue Native gel electrophoresis Western blotting and immunodetection.

Strumentazione

Denominazione

ChemidocTM MP Imaging System (BioRad)
Imaging System Typhoon FLA 9500
Cryostat microtome (Microm)
Bidimensional electrophoresis apparatus
Real Time PCR apparatus QuantStudio7Flex
Cell Cultures Facility
FPLC System for protein chromatography
Calorimeter MicroCal™ iTC200 System
Fluorescence Microscopes

Struttura ove la strumentazione è allocata

Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment
Biosciences, Biotechnologies and Environment

Responsabile

A.M.S. Lezza
F. Bruni
V. Pesce
A.M.S. Lezza
F. Bruni
P. Loguercio Polosa
P. Loguercio Polosa
V. Pesce
V. Pesce

Pubblicazioni

  1. 1.
    Differences in liver TFAM binding to mtDNA and mtDNA damage between aged and extremely aged rats. Chimienti G, Picca A, Fracasso F, Marzetti E, Calvani R, Leeuwenburgh C, Russo F, Lezza AMS, Pesce V Int J Mol Sci. 2019 May 27;20(10):2601. doi: 10.3390/ijms20102601. PMID: 31137890 
  2. 2.
    Increased TFAM binding to mtDNA damage hot spots is associated with mtDNA loss in aged rat heart. Chimienti G, Picca A, Sirago G, Fracasso F, Calvani R, Bernabei R, Russo F, Carter CS, Leeuwenburgh C, Pesce V, Marzetti E, Lezza AMS Free Radic Biol Med. 2018 Aug 20; 124:447-453. doi:10.1016/j.freeradbiomed.2018.06.041.
  3. 3.
    Aging and calorie restriction oppositely affect mitochondrial biogenesis through TFAM binding at both origins of mitochondrial DNA replication in rat liver. Picca A, Pesce V, Fracasso F, Joseph AM, Leeuwenburgh C, Lezza AMS PLoS ONE, 2013, 8(9): e74644; doi:10.1371/journal.pone.0074644
  4. 4.
    Growth hormone secretagogues hexarelin and JMV2894 protect skeletal muscle from mitochondrial damages in a rat model of cisplatin-induced cachexia. Sirago G, Conte E, Fracasso F, Cormio A, Fehrentz JA, Martinez J, Musicco C, Camerino GM, Fonzino A, Rizzi L, Torsello A, Lezza AMS, Liantonio A, Cantatore P, Pesce V. Sci Rep. 2017 Oct 12;7(1):13017. doi: 10.1038/s41598-017-13504-y. PMID: 29026190 
  5. 5.
    Clinical, biochemical, and genetic features associated with VARS2-related mitochondrial disease. Bruni F, Di Meo I, Bellacchio E, Webb BD, McFarland R, Chrzanowska-Lightowlers ZMA, He L, Skorupa E, Moroni I, Ardissone A, Walczak A, Tyynismaa H, Isohanni P, Mandel H, Prokisch H, Haack T, Bonnen PE, Enrico B, Pronicka E, Ghezzi D, Taylor RW, Diodato D. Hum Mutat. 2018 Apr;39(4):563-578. doi: 10.1002/humu.23398.
  6. 6.
    Toward the Standardization of Mitochondrial Proteomics: The Italian Mitochondrial Human Proteome Project Initiative Alberio T, Pieroni L, Ronci M, Banfi C, Bongarzone I, Bottoni P, Brioschi M, Caterino M, Chinello C, Cormio A, Cozzolino F, Cunsolo V, Fontana S, Garavaglia B, Giusti L, Greco V, Lucacchini A, Maffioli E, Magni F, Monteleone F, Monti M, Monti V, Musicco C, Petrosillo G, Porcelli V, Saletti R, Scatena R, Soggiu A, Tedeschi G, Zilocchi M, Roncada P, Urbani A, Fasano M J Proteome Res. 2017 16: 4319-4329.28. doi: 10.1021/acs.jproteome.7b00350
  7. 7.
    Inhibition of Lon protease by triterpenoids alters mitochondria and is associated to cell death in human cancer cells. Gibellini L, Pinti M, Bartolomeo R, De Biasi S, Cormio A, Musicco C, Carnevale G, Pecorini S, Nasi M, De Pol A, Cossarizza A. Oncotarget 2015 6: 25466-25483 doi: 10.18632/oncotarget.4510
  8. 8.
    Cigarette toxicity triggers Leber’s hereditary optic neuropathy by affecting mtDNA copy number, oxidative phosphorylation and ROS detoxification pathways. Giordano L., Deceglie S., d’Adamo P., Valentino M.L., La Morgia C., Fracasso F., Roberti M., Cappellari M., Petrosillo G., Ciaravolo S., Parente D., Giordano C., Maresca A., Iommarini L., Del Dotto V., Ghelli A.M., Salomao S.R., Berezovsky A., Belfort Jr R., Sadun A.A., Carelli V., Loguercio Polosa P., Cantatore P Cell Death and Disease (2015) 6, e2021 doi: 10.1038/cddis.2015.364.
  9. 9.
    The sea urchin mitochondrial transcription factor a binds and bends DNA efficiently despite its unusually short c-terminal tail. Malarkey C.S., Lionetti C., Deceglie S., Roberti M., Churchill M.E.A., Cantatore P., Loguercio Polosa P. Mitochondrion (2016) 29, 1-6. doi: 10.1016/j.mito.2016.04.004.
  10. 10.
    Mice lacking the mitochondrial exonuclease MGME1 accumulate mtDNA deletions without developing progeria Matic S, Jiang M,. Nicholls TJ,. Uhler JP, Dirksen-Schwanenland C, Loguercio Polosa P, Simard M-L, Li X, Atanassov I, Rackham O, Filipovska A, Stewart JB, Falkenberg M, Larsson N-G, Milenkovic D Nature Communications (2018) 9(1):1202. doi: 10.1038/s41467-018-03552-x.

Dottorati di ricerca

Componente UO

Pesce V.
Lezza A.M.S.
Bruni F.
Cormio A.

Dottorato di ricerca

BIOSCIENZE E BIOTECNOLOGIE
BIOSCIENZE E BIOTECNOLOGIE
BIOSCIENZE E BIOTECNOLOGIE
BIOSCIENZE E BIOTECNOLOGIE

Coordinatore

Prof. G. Valenti
Prof. G. Valenti
Prof. G. Valenti
Prof. G. Valenti

Sede

University of Bari
University of Bari
University of Bari
University of Bari