Palmieri Luigi

Responsabile dell'U.O.

Cognome e Nome

Palmieri Luigi

Qualifica

PO

Dipartimento

Biosciences, Biotechnologies and Environment

Settore scientifico disciplinare

BIO/10

E-mail

luigi.palmieri@farmbiol.uniba.it

Telefono

Personale strutturato

Cognome e Nome

Fiermonte Giuseppe

Qualifica

PO

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Gennaro Agrimi

Qualifica

PA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Pasquale Scarcia

Qualifica

PA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Vozza Angelo

Qualifica

PA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Lasorsa Francesco Massimo

Qualifica

PA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Pisano Isabella

Qualifica

RTDB

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Marobbio Carlo Marya Thomas

Qualifica

PA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Personale non strutturato

Cognome e Nome

Gorgoglione Ruggiero

Qualifica

RTDA

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Cognome e Nome

Biundo Antonino

Qualifica

Post-doc

Dipartimento

Biosciences, Biotechnologies and Environment

Ente di appartenenza

University of Bari

Linee di ricerca

• Identification and functional characterization of mitochondrial carrier coding genes. • Metabolic engineering of microorganisms for the production of chemical compounds and recombinant proteins of industrial interest • Biocatalysis for the production of molecules of industrial interest • Targeted and untargeted metabolomics using GC-MS and LC-MS • Cloning and heterologous and homologous overexpression in E. coli and S. cerevisiae of genes encoding mitochondrial transporters. • Gene silencing and overexpression in mammalian cells of genes encoding mitochondrial transporters, by transfection with plasmids, siRNA and infection of cultured cells with viral vectors. • Identification of mutations in patients with mitochondrial transporter deficiency. • Analysis of cellular and mitochondrial physiological parameters by flow cytometry. Bioenergetics and metabolism of ROS in cellular models of neurodegenerative diseases. • Phenotypic analysis of mutant yeast strains.

Tecnologie in uso dall'UO

  1. 1.
    Spectrophotometry and Spectrofluorimentry.
  2. 2.
    Mass spectrometry coupled to GC and LC.
  3. 3.
    Flow cytometry
  4. 4.
    Mammalian cell cultures.
  5. 5.
    Fermentation processes
  6. 6.
    Gene clonin, overexpression. Recombinant protein production and purification
  7. 7.
    Metabolic engineering of microbial cell factories
  8. 8.
    Radioisotopic techniques.
  9. 9.
    Real-Time PCR
  10. 10.
    Targeted e untargeted metabolomics

Strumentazione

Denominazione

Attune Nxt Acoustic Focusing Cytometer.
MS Micromass Quattro Premier (spettrometro di massa) GC-MS
Waters Alliance HPLC system
ADI 1010 Bio Controller for in situ Sterilizable BIO BENCH reactors (fermentatore)
QS3 Real Time PCR System –Applied Biosystem

Struttura ove la strumentazione è allocata

Stanza 211 (1° pianoex Dip. Farmaco-Biologico)
Stanza 211 (1° pianoex Dip. Farmaco-Biologico)
Stanza 211 (1° pianoex Dip. Farmaco-Biologico)
Stanza 211 (1° pianoex Dip. Farmaco-Biologico)
Locale sotterraneo ex Dip. Farmaco-Biologico)

Responsabile

Pisano Isabella
Gennaro Agrimi
Luigi Palmieri
Iacobazzi Vito
Pasquale Scarcia

Pubblicazioni

  1. 1.
    Biundo A, Stamm A, Gorgoglione R, Syrén PO, Curia S, Hauer B, Capriati V, Vitale P, Perna F, Agrimi G, Pisano I. Regio- and stereoselective biocatalytic hydration of fatty acids from waste cooking oils en route to hydroxy fatty acids and bio-based polyesters. Enzyme Microb Technol. 2023 Feb;163:110164. doi: 10.1016/j.enzmictec.2022.110164. Epub 2022 Nov 25. PMID: 36455467.
  2. 2.
    Shahroor MA, Lasorsa FM, Porcelli V, Dweikat I, Di Noia MA, Gur M, Agostino G, Shaag A, Rinaldi T, Gasparre G, Guerra F, Castegna A, Todisco S, Abu-Libdeh B, Elpeleg O, Palmieri L. PNC2 (SLC25A36) Deficiency Associated With the Hyperinsulinism/Hyperammonemia Syndrome. J Clin Endocrinol Metab. 2022 Apr 19;107(5):1346-1356. doi: 10.1210/clinem/dgab932. PMID: 34971397.
  3. 3.
    Seccia R, De Santis S, Di Noia MA, Palmieri F, Miniero DV, Marmo R, Paradies E, Santoro A, Pierri CL, Palmieri L, Marobbio CMT, Vozza A. Citrate Regulates the Saccharomyces cerevisiae Mitochondrial GDP/GTP Carrier (Ggc1p) by Triggering Unidirectional Transport of GTP. J Fungi (Basel). 2022 Jul 29;8(8):795. doi: 10.3390/jof8080795. PMID: 36012783; PMCID: PMC9410265.
  4. 4.
    Yuzbasheva EY, Scarcia P, Yuzbashev TV, Messina E, Kosikhina IM, Palmieri L, Shutov AV, Taratynova MO, Amaro RL, Palmieri F, Sineoky SP, Agrimi G. Engineering Yarrowia lipolytica for the selective and high-level production of isocitric acid through manipulation of mitochondrial dicarboxylate-tricarboxylate carriers. Metab Eng. 2021 May;65:156-166. doi: 10.1016/j.ymben.2020.11.001. Epub 2020 Nov 5. PMID: 33161142.
  5. 5.
    Wierckx N, Agrimi G, Lübeck PS, Steiger MG, Mira NP, Punt PJ. Metabolic specialization in itaconic acid production: a tale of two fungi. Curr Opin Biotechnol. 2020 Apr;62:153-159. doi: 10.1016/j.copbio.2019.09.014. Epub 2019 Nov 2. PMID: 31689647.
  6. 6.
    Raho S, Capobianco L, Malivindi R, Vozza A, Piazzolla C, De Leonardis F, Gorgoglione R, Scarcia P, Pezzuto F, Agrimi G, Barile SN, Pisano I, Reshkin SJ, Greco MR, Cardone RA, Rago V, Li Y, Marobbio CMT, Sommergruber W, Riley CL, Lasorsa FM, Mills E, Vegliante MC, De Benedetto GE, Fratantonio D, Palmieri L, Dolce V, Fiermonte G. KRAS-regulated glutamine metabolism requires UCP2-mediated aspartate transport to support pancreatic cancer growth. Nat Metab. 2020 Dec;2(12):1373-1381. doi: 10.1038/s42255-020-00315-1. Epub 2020 Nov 23. PMID: 33230296.
  7. 7.
    Girardi E, Agrimi G, Goldmann U, Fiume G, Lindinger S, Sedlyarov V, Srndic I, Gürtl B, Agerer B, Kartnig F, Scarcia P, Di Noia MA, Liñeiro E, Rebsamen M, Wiedmer T, Bergthaler A, Palmieri L, Superti-Furga G. Epistasis-driven identification of SLC25A51 as a regulator of human mitochondrial NAD import. Nat Commun. 2020 Dec 1;11(1):6145. doi: 10.1038/s41467-020-19871-x. PMID: 33262325; PMCID: PMC7708531.
  8. 8.
    Yuzbasheva EY, Agrimi G, Yuzbashev TV, Scarcia P, Vinogradova EB, Palmieri L, Shutov AV, Kosikhina IM, Palmieri F, Sineoky SP. The mitochondrial citrate carrier in Yarrowia lipolytica: Its identification, characterization and functional significance for the production of citric acid. Metab Eng. 2019 Jul;54:264-274. doi: 10.1016/j.ymben.2019.05.002. Epub 2019 May 6. PMID: 31071446.
  9. 9.
    Santoro A, Anjomani Virmouni S, Paradies E, Villalobos Coa VL, Al-Mahdawi S, Khoo M, Porcelli V, Vozza A, Perrone M, Denora N, Taroni F, Merla G, Palmieri L, Pook MA, Marobbio CMT. Effect of diazoxide on Friedreich ataxia models. Hum Mol Genet. 2018 Mar 15;27(6):992-1001. doi: 10.1093/hmg/ddy016. PMID: 29325032.
  10. 10.
    Zaltsman Y, Shachnai L, Yivgi-Ohana N, Schwarz M, Maryanovich M, Houtkooper RH, Vaz FM, De Leonardis F, Fiermonte G, Palmieri F, Gillissen B, Daniel PT, Jimenez E, Walsh S, Koehler CM, Roy SS, Walter L, Hajnóczky G, Gross A. MTCH2/MIMP is a major facilitator of tBID recruitment to mitochondria. Nat Cell Biol. 2010 Jun;12(6):553-562. doi: 10.1038/ncb2057. Epub 2010 May 2. PMID: 20436477; PMCID: PMC4070879.

Dottorandi di ricerca

Componente UO

Luigi Palmieri
Giuseppe Fiermonte
Gennaro Agrimi
Pasquale Scarcia
Angelo Vozza
Carlo Marya Thomas Marobbio
Lasorsa Francesco Massimo

Dottorato di ricerca

Biosciences and Biotechnologies
Biosciences and Biotechnologies
Biosciences and Biotechnologies
Biosciences and Biotechnologies
Biosciences and Biotechnologies
Biosciences and Biotechnologies
Biosciences and Biotechnologies

Coordinatore

Giovanna Valenti
Giovanna Valenti
Giovanna Valenti
Giovanna Valenti
Giovanna Valenti
Giovanna Valenti
Giovanna Valenti

Sede

Bari
Bari
Bari
Bari
Bari
Bari
Bari