Responsabile dell'U.O.
Cognome e Nome
Masci Stefania
Qualifica
PA
Dipartimento
Agricultural and Forestry Science (DAFNE)
Settore scientifico disciplinare
AGR/07
masci@unitus.it
Telefono
+390761357255; +393288641241
Personale strutturato
Cognome e Nome
Lafiandra Domenico
Qualifica
PO
Dipartimento
DAFNE
Ente di appartenenza
Tuscia University
Cognome e Nome
Sestili Francesco
Qualifica
Ricercatore
Dipartimento
DAFNE
Ente di appartenenza
Tuscia University
Personale non strutturato
Cognome e Nome
Botticella Ermelinda
Qualifica
Post-doc
Dipartimento
DAFNE
Ente di appartenenza
Tuscia University
Cognome e Nome
Lupi Roberta
Qualifica
Post-doc
Dipartimento
DAFNE
Ente di appartenenza
Tuscia University
Cognome e Nome
Crmerlengo Francesco
Qualifica
Ph.D student
Dipartimento
DAFNE
Ente di appartenenza
Tuscia University
Linee di ricerca
The research is directed at understanding the genetic and molecular bases of wheat technological and nutritional properties, and at the manipulation of wheat kernel composition in order to obtain new wheat genotypes with improved properties.
In particular, the research is focused on wheat kernel proteins related to dough technological quality (gluten proteins), to adverse reactions to wheat (such as celiac disease, wheat sensitivity, wheat allergies), and on the manipulation of wheat starch composition, by altering the activity of genes related to starch metabolism
Tecnologie in uso dall'UO
- 1.Chemical mutagenesis
- 2.Durum wheat TILLING platform
- 3.Gel-based proteomics
- 4.Immunoassays by using sera of allergic patients and specific antibodies
- 5.Rapid Visco Analyser
- 6.Gene silencing by RNAi
- 7.Real Time PCR
- 8.SNP genotyping
Strumentazione
Denominazione
LightScanner (Idaho)
Gel-based proteomics (2D electrophoresis)
DIGE system
HPLC
LightCycler 480 (Roche)
Struttura ove la strumentazione è allocata
DAFNE
DAFNE
CGA
DAFNE
CGA
Responsabile
Sestili/Lafiandra
Masci
Masci
Masci/Lafiandra
Simeone
Pubblicazioni
- 1.Giuliani M.M, Palermo C., De Santis M.A., Mentana A., Pompa M., Giuzio L., Masci S., Centonze D., Flagella Z., 2015. Differential expression of durum wheat gluten proteome under water stress during grain filling. Journal of Agricultural and Food Chemistry 63(29):6501–6512
- 2.Masci S., Laino P., Janni M., Botticella E., Di Carli M., Benvenuto E., Danieli P.P., Lilley K.S., Lafiandra D., D’Ovidio R., 2015. Analysis of quality-related parameters in mature kernels of PGIP transgenic bread wheat infected with Fusarium graminearum. Journal of Agricultural and Food Chemistry 63(15):3962-3969
- 3.Rakszegi, M., Kisgyörgy, B.N., Kiss, T., Sestili, F., Láng, L., Lafiandra, D., Bedö, Z. Development and characterization of high-amylose wheat lines, 2015. Starch/Staerke, 67 (3-4), pp. 247-254
- 4.Sestili, F., Palombieri, S., Botticella, E., Mantovani, P., Bovina, R., Lafiandra, D. TILLING mutants of durum wheat result in a high amylose phenotype and provide information on alternative splicing mechanisms, 2015. Plant Science, 233:127-133
- 5.Egidi E., Sestili F., Janni M., D’Ovidio R., Lafiandra D., Ceriotti A., Vensel W.H., Kasarda D.D., Masci S., 2014. An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm. BMC Plant Biology BMC Plant Biology, 14:64
- 6.Lupi R., Masci S., Rogniaux H., Tranquet O., Brossard C., Lafiandra D., Moneret-Vautrin A.D., Denery-Papini S., Larré C., 2014. Assessment of the allergenicity of soluble fractions from GM and commercial genotypes of wheats. Journal of Cereal Science 60:179-186
- 7.Sparla F., Falini G., Botticella E., Pirone C., Talamè V., Bovina R., Salvi S., Tuberosa R., Sestili F., Trost P., 2014. New starch phenotypes produced by TILLING in barley. PLoS ONE, 9(10):e107779.
- 8.Wang, X., Feng, B., Xu, Z., Sestili, F., Zhao, G., Xiang, C., Lafiandra, D., Wang, T. Identification and characterization of granule bound starch synthase I (GBSSI) gene of tartary buckwheat (Fagopyrum tataricum Gaertn.), 2014. Gene, 534:229-235.
- 9.Lupi R., Denery-Papini S., Rogniaux H., Lafiandra D., Rizzi C., De Carli M., Moneret-Vautrin A.D., Masci S., Larré C., 2013. How much does transgenesis affect wheat allergenicity? Assessment in two GM lines over-expressing endogenous genes. Journal of Proteomics 80:281-291
- 10.Sestili F., Paoletti F., Botticella E., Masci S., Saletti R., Muccilli V., Lafiandra D., 2013. Comparative proteomic analysis of kernel proteins of two high amylose transgenic durum wheat lines obtained by biolistic and Agrobacterium-mediated transformations. Journal of Cereal Science 58:15-22
Dottorandi di ricerca
Componente UO
Masci Stefania
Masci Stefania
Lafiandra Domenico
Sestili
Dottorato di ricerca
Plant Biotechnology (up to the XXVIII Cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)
Coordinatore
Masci Stefania
Lafiandra Domenico
Lafiandra Domenico
Lafiandra Domenico
Sede
Viterbo
Viterbo
Viterbo
Viterbo