Masci Stefania

Responsabile dell'U.O.

Cognome e Nome

Masci Stefania

Qualifica

PA

Dipartimento

Agricultural and Forestry Science (DAFNE)

Settore scientifico disciplinare

AGR/07

E-mail

masci@unitus.it

Telefono

+390761357255; +393288641241

Personale strutturato

Cognome e Nome

Lafiandra Domenico

Qualifica

PO

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Sestili Francesco

Qualifica

Ricercatore

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Personale non strutturato

Cognome e Nome

Botticella Ermelinda

Qualifica

Post-doc

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Lupi Roberta

Qualifica

Post-doc

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Crmerlengo Francesco

Qualifica

Ph.D student

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Linee di ricerca

The research is directed at understanding the genetic and molecular bases of wheat technological and nutritional properties, and at the manipulation of wheat kernel composition in order to obtain new wheat genotypes with improved properties. In particular, the research is focused on wheat kernel proteins related to dough technological quality (gluten proteins), to adverse reactions to wheat (such as celiac disease, wheat sensitivity, wheat allergies), and on the manipulation of wheat starch composition, by altering the activity of genes related to starch metabolism

Tecnologie in uso dall'UO

  1. 1.
    Chemical mutagenesis
  2. 2.
    Durum wheat TILLING platform
  3. 3.
    Gel-based proteomics
  4. 4.
    Immunoassays by using sera of allergic patients and specific antibodies
  5. 5.
    Rapid Visco Analyser
  6. 6.
    Gene silencing by RNAi
  7. 7.
    Real Time PCR
  8. 8.
    SNP genotyping

Strumentazione

Denominazione

LightScanner (Idaho)
Gel-based proteomics (2D electrophoresis)
DIGE system
HPLC
LightCycler 480 (Roche)

Struttura ove la strumentazione è allocata

DAFNE
DAFNE
CGA
DAFNE
CGA

Responsabile

Sestili/Lafiandra
Masci
Masci
Masci/Lafiandra
Simeone

Pubblicazioni

  1. 1.
    Giuliani M.M, Palermo C., De Santis M.A., Mentana A., Pompa M., Giuzio L., Masci S., Centonze D., Flagella Z., 2015. Differential expression of durum wheat gluten proteome under water stress during grain filling. Journal of Agricultural and Food Chemistry 63(29):6501–6512
  2. 2.
    Masci S., Laino P., Janni M., Botticella E., Di Carli M., Benvenuto E., Danieli P.P., Lilley K.S., Lafiandra D., D’Ovidio R., 2015. Analysis of quality-related parameters in mature kernels of PGIP transgenic bread wheat infected with Fusarium graminearum. Journal of Agricultural and Food Chemistry 63(15):3962-3969
  3. 3.
    Rakszegi, M., Kisgyörgy, B.N., Kiss, T., Sestili, F., Láng, L., Lafiandra, D., Bedö, Z. Development and characterization of high-amylose wheat lines, 2015. Starch/Staerke, 67 (3-4), pp. 247-254
  4. 4.
    Sestili, F., Palombieri, S., Botticella, E., Mantovani, P., Bovina, R., Lafiandra, D. TILLING mutants of durum wheat result in a high amylose phenotype and provide information on alternative splicing mechanisms, 2015. Plant Science, 233:127-133
  5. 5.
    Egidi E., Sestili F., Janni M., D’Ovidio R., Lafiandra D., Ceriotti A., Vensel W.H., Kasarda D.D., Masci S., 2014. An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm. BMC Plant Biology BMC Plant Biology, 14:64
  6. 6.
    Lupi R., Masci S., Rogniaux H., Tranquet O., Brossard C., Lafiandra D., Moneret-Vautrin A.D., Denery-Papini S., Larré C., 2014. Assessment of the allergenicity of soluble fractions from GM and commercial genotypes of wheats. Journal of Cereal Science 60:179-186
  7. 7.
    Sparla F., Falini G., Botticella E., Pirone C., Talamè V., Bovina R., Salvi S., Tuberosa R., Sestili F., Trost P., 2014. New starch phenotypes produced by TILLING in barley. PLoS ONE, 9(10):e107779.
  8. 8.
    Wang, X., Feng, B., Xu, Z., Sestili, F., Zhao, G., Xiang, C., Lafiandra, D., Wang, T. Identification and characterization of granule bound starch synthase I (GBSSI) gene of tartary buckwheat (Fagopyrum tataricum Gaertn.), 2014. Gene, 534:229-235.
  9. 9.
    Lupi R., Denery-Papini S., Rogniaux H., Lafiandra D., Rizzi C., De Carli M., Moneret-Vautrin A.D., Masci S., Larré C., 2013. How much does transgenesis affect wheat allergenicity? Assessment in two GM lines over-expressing endogenous genes. Journal of Proteomics 80:281-291
  10. 10.
    Sestili F., Paoletti F., Botticella E., Masci S., Saletti R., Muccilli V., Lafiandra D., 2013. Comparative proteomic analysis of kernel proteins of two high amylose transgenic durum wheat lines obtained by biolistic and Agrobacterium-mediated transformations. Journal of Cereal Science 58:15-22

Dottorandi di ricerca

Componente UO

Masci Stefania
Masci Stefania
Lafiandra Domenico
Sestili

Dottorato di ricerca

Plant Biotechnology (up to the XXVIII Cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)

Coordinatore

Masci Stefania
Lafiandra Domenico
Lafiandra Domenico
Lafiandra Domenico

Sede

Viterbo
Viterbo
Viterbo
Viterbo