Masci Stefania

Responsabile dell'U.O.

Cognome e Nome

Masci Stefania

Qualifica

PA

Dipartimento

Agricultural and Forestry Science (DAFNE)

Settore scientifico disciplinare

AGR/07

E-mail

masci@unitus.it

Telefono

+390761357255; +393288641241

Personale strutturato

Cognome e Nome

Lafiandra Domenico

Qualifica

PO

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Sestili Francesco

Qualifica

PA

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Personale non strutturato

Cognome e Nome

Palombieri Samuela

Qualifica

RTDA

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Metelli Giulio

Qualifica

Post-doc

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Frittelli Arianna

Qualifica

Post-doc

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Bonarrigo Marco

Qualifica

Ph.D student

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

D’Attilia Chiara

Qualifica

Ph.D student

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Bruno Giuliana

Qualifica

Ph.D student

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Cognome e Nome

Ali Ehtasham

Qualifica

Ph.D student

Dipartimento

DAFNE

Ente di appartenenza

Tuscia University

Linee di ricerca

The research is directed at understanding the genetic and molecular bases of wheat technological and nutritional properties, and at the manipulation of wheat kernel composition in order to obtain new wheat genotypes with improved properties. In particular, the research is focused on wheat kernel proteins related to dough technological quality (gluten proteins), to adverse reactions to wheat (such as celiac disease, wheat sensitivity, wheat allergies), and to the increase of nutritional properties, by using classical and biotechnological approaches including genome editing by CRISPR-Cas9

Tecnologie in uso dall'UO

  1. 1.
    Chemical mutagenesis
  2. 2.
    Durum wheat TILLING platform
  3. 3.
    Gel-based proteomics
  4. 4.
    Immunoassays by using sera of allergic patients and specific antibodies
  5. 5.
    Rapid Visco Analyser
  6. 6.
    Gene silencing by RNAi and CRISPR-Cas9
  7. 7.
    Real Time PCR
  8. 8.
    SNP genotyping

Strumentazione

Denominazione

LightScanner (Idaho)
Gel-based proteomics (2D electrophoresis)
Biomark X9
HPLC
LightCycler 480 (Roche)

Struttura ove la strumentazione è allocata

DAFNE
DAFNE
CGA
DAFNE
CGA

Responsabile

Sestili/Masci
Sestili/Masci
Sestili/Masci
Sestili/Masci
Sestili/Masci

Pubblicazioni

  1. 1.
    Coppa E., Celletti S., Sestili F., Mimmo T., Garcia Molina M.D., Cesco S., Astolfi S. 2023. Interaction between sulfate and selenate in tetraploid wheat (Triticum turgidum L.) genotypes. International Journal of Molecular Science 24, 5443. https://doi.org/10.3390/ijms24065443
  2. 2.
    Di Rosa, C.; De Arcangelis, E.; Vitelli, V.; Crucillà, S.; Angelicola, M.; Trivisonno, M.C.; Sestili, F.; Blasi, E.; Cicatiello, C.; Lafiandra, D.; Masci S., Messia M.C., De Gara L., Marconi E., Khazraiet Y.M., 2023. Effect of Three Bakery Products Formulated with High-Amylose Wheat Flour on Post-Prandial Glycaemia in Healthy Volunteers. Foods 2023, 12, 319. https://doi.org/10.3390/foods12020319
  3. 3.
    Emide D., Magni C., Saitta F., Cardone G., Botticella E., Fessas D., Iametti S., Lafiandra D., Sestili F., Marti A., Barbirolia A. 2023. Molecular insights into the role of amylose/amylopectin ratio on gluten protein organization. Food Chemistry, 404, 134675. https://doi.org/10.1016/j.foodchem.2022.134675
  4. 4.
    Francesconi S., Ronchetti R., Camaioni E., Giovagnoli S., Sestili F., Palombieri S., Balestra G. 2023. Boosting immunity and management against wheat Fusarium diseases by a sustainable, circular nanostructured delivery platform. Plants 12(6), 1223. https://doi.org/10.3390/plants12061223
  5. 5.
    Frittelli A, Botticella E, Palombieri S, Masci S, Celletti S, Fontanella MC, Astolfi S, De Vita P, Volpato M and Sestili F (2023) The suppression of TdMRP3 genes reduces the phytic acid and increases the nutrient accumulation in durum wheat grain. Front. Plant Sci. 14:1079559. doi: 10.3389/fpls.2023.1079559
  6. 6.
    Frittelli Arianna; Samuela Palombieri; Giulia Quagliata; Silvia Celletti; Stefania Astolfi; Ermelinda Botticella; Stefania Masci; Mirko Volpato; Francesco Sestili, 2023. The silencing of TdIPK1 genes enhances micronutrient concentration in durum wheat grain. Current Plant Biology 35-36: 100309. https://doi.org/10.1016/j.cpb.2023.100309
  7. 7.
    Palombieri, S., Bonarrigo, M., Cammerata, A., Quagliata, G., Astolfi, S., Lafiandra, D., Sestili, F., & Masci, S., 2023. Characterization of Triticum turgidum sspp. Durum, turanicum and polonicum grown in Central Italy in relation to technological and nutritional aspects. Frontiers in Plant Science, 14, 1269212. https://doi.org/10.3389/fpls.2023.1269212
  8. 8.
    Pasqualone A., Palombieri S., Koksel H., Summo C., De Vita P., Sestili F. 2023. Milling and bread-making performance of the new soft kernel durum wheat variety Faridur. International Journal of Food Science and Technology. https://doi.org/10.1111/ijfs.16200
  9. 9.
    Quagliata G., Abdirad S., Celletti S., Sestili F., Astolfi S. 2023 Screening of Triticum turgidum genotypes for tolerance to drought stress. Plant Physiology and Biochemistry, 194, 271-280. https://doi.org/10.1016/j.plaphy.2022.11.025
  10. 10.
    Sissons M., Palombieri S., Sestili F., Lafiandra D. 2023. Impact of Variation in Amylose Content on Durum Wheat cv. Svevo Technological and Starch Properties. Foods, 12(22), 4112. https://doi.org/10.3390/foods12224112
  11. 11.
    Tafuri Andrea, Zuccaro Melania, Ravaglia Stefano, Pirona Raul, Masci Stefania, Sestili Francesco, Lafiandra Domenico, Ceriotti Aldo, Baldoni Elena (2023) Exploring variability in free asparagine seed content in bread wheat (Triticum aestivum L.) Italian cultivars to reduce acrylamide-forming potential. Plants 2023, 12, 1349. https://doi.org/10.3390/plants12061349

Dottorati di ricerca

Componente UO

Masci Stefania
Masci Stefania
Lafiandra Domenico
Sestili Francesco

Dottorato di ricerca

Plant Biotechnology (up to the XXVIII Cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)
Plant and Animal Science (starting from the XXIX cycle)

Coordinatore

Masci Stefania
Lafiandra Domenico
Lafiandra Domenico
Lafiandra Domenico

Sede

Viterbo
Viterbo
Viterbo
Viterbo