Marconi Peggy

Responsabile dell'U.O.

Cognome e Nome

Marconi Peggy

Qualifica

PO

Dipartimento

Scienze chimiche e farmaceutiche e Agrarie

Settore scientifico disciplinare

MED 07

E-mail

peggy.marconi@unife.it

Telefono

0532 455381/0532 455403

Personale strutturato

Cognome e Nome

Gavioli Riccardo

Qualifica

PO

Dipartimento

Scienze chimiche e farmaceutiche e Agrarie

Ente di appartenenza

Università di Ferrara

Cognome e Nome

Gallerani Eleonora

Qualifica

Technician

Dipartimento

Scienze della Vita e Biotecnologie

Ente di appartenenza

Università di Ferrara

Cognome e Nome

Buratto Mattia

Qualifica

Technician

Dipartimento

Scienze chimiche e farmaceutiche e Agrarie

Ente di appartenenza

Università di Ferrara

Personale non strutturato

Linee di ricerca

Study of the function of the principal Herpes simplex virus (HSV) glycoproteins. Construction of stable cell lines expressing HSV-1 and HSV-2 recombinant proteins, for diagnostic and vaccine purposes. Expression of HSV recombinant proteins Construction of recombinant plasmids and proteins for anti HSV vaccine. Construction of herpetic vectors with modified cellular targeting. Development of mono and polyvalent vaccines by using a new generation of HSV-1 vectors. Use of herpetic vectors for siRNA delivery. Development of HSV-1 vectors for the study and gene therapy of pathologies of the Central Nervous System. Development of HSV-1 oncolytic and non-replicative vectors for cancer gene therapy. Development of new viral challenge models to study antiviral vaccines. Study of antiviral drugs. Development of new vaccines against AIDS based on the HIV-1 Tat protein or Tat-derived peptides. Development of immunotherapeutic strategies to increase responses to vaccines, tumors and viral infections Study of immunosenescence Study of immunometabolism

Tecnologie in uso dall'UO

  1. 1.
    ELISPOT
  2. 2.
    PCR
  3. 3.
    Flow cytometry
  4. 4.
    tissue cultures
  5. 5.
    methods to study viruses
  6. 6.
    transfection and transduction
  7. 7.
    cloning techniques
  8. 8.
    construction of viral vectors
  9. 9.
    animal models for oncologic studies
  10. 10.
    animal model for vaccine studies
  11. 11.
    Culture of T cytotoxic lymphocytes
  12. 12.
    Protein purification
  13. 13.
    Enzymatic assays
  14. 14.
    Study of metabolic properties of immune cells
  15. 15.
    Analysis of in vitro and in vivo T and B cell responses

Strumentazione

Denominazione

ELISPOT reader
VICTOR
FACScantoII
3D
Automated Droplet Generator+ PX1TM PCR Plate Sealer+ QX200TM Droplet Reader + Quanta SoftTM Software versione 1.7

Struttura ove la strumentazione è allocata

Laboratorio grandi attrezzature (NIB CPA)
(NIB CPA)
SVEB/Dipartimento di scienz. chim. e farmac. e Agr.
(Lab grandi attrezzature –NIB CPA)
SVEB

Responsabile

Prof. R. Gavioli
Proff. F. Di Virgilio, R.Gavioli
Proff. M. Borgatti, R. Gavioli
Proff. Bernardi, Gambari, Gavioli, Pinotti, Bellini
Prof.ssa M. Borgatti

Pubblicazioni

  1. 1.
    Nicoli F, Gallerani E, Skarlis C, Sicurella M, Cafaro A, Ensoli B, Caputo A, Marconi PC, Gavioli R. (2016). Systemic immunodominant CD8 responses with an effector-like phenotype are induced by intravaginal immunization with attenuated HSV vectors expressing HIV Tat and mediate protection against HSV infection. Vaccine. 34(19):2216-24.
  2. 2.
    Marconi P, Fraefel C, Epstein AL. (2015). Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy. Methods Mol Biol. 1254:269-93.
  3. 3.
    Sicurella M, Nicoli F, Gallerani E, Volpi I, Berto E, Finessi V, Destro F, Manservigi R, Cafaro A, Ensoli B, Caputo A, Gavioli R, Marconi PC. (2014). An attenuated herpes simplex virus type 1 (HSV1) encoding the HIV-1 Tat protein protects mice from a deadly mucosal HSV1 challenge. PLoS One. 17;9(7):e100844.
  4. 4.
    Nicoli F, Finessi V, Sicurella M, Rizzotto L, Gallerani E, Destro F, Cafaro A, Marconi P, Caputo A, Ensoli B, Gavioli R. (2013). The HIV-1 Tat protein induces the activation of CD8+ T cells and affects in vivo the magnitude and kinetics of antiviral responses. PLoS One. 4;8(11):e77746.
  5. 5.
    Edelmann SL, Marconi P, Brocker T. (2011). Peripheral T cells re-enter the thymus and interfere with central tolerance induction. J Immunol. 15;186(10):5612-9
  6. 6.
    Marconi P, Argnani R, Epstein AL, Manservigi R. (2009). HSV as a vector in vaccine development and gene therapy. Adv Exp Med Biol. 655:118-44.
  7. 7.
    Paradiso B, Marconi P, Zucchini S, Berto E, Binaschi A, Bozac A, Buzzi A, Mazzuferi M, Magri E, Navarro Mora G, Rodi D, Su T, Volpi I, Zanetti L, Marzola A, Manservigi R, Fabene PF, Simonato M. (2009). Localized delivery of fibroblast growth factor-2 and brain-derived neurotrophic factor reduces spontaneous seizures in an epilepsy model. Proc Natl Acad Sci U S A 28;106(17):7191-6.
  8. 8.
    Dresch C, Edelmann SL, Marconi P, Brocker T. (2008) Lentiviral-mediated transcriptional targeting of dendritic cells for induction of T cell tolerance in vivo. J Immunol. 181(7):4495-506.
  9. 9.
    Marconi P, Argnani R, Berto E, Epstein AL, Manservigi R. (2008) HSV as a vector in vaccine development and gene therapy. Hum Vaccin.;4(2):91-105.
  10. 10.
    Luckashenak N, Schroeder S, Endt K, Schmidt D, Mahnke K, Bachmann MF, Marconi P, Deeg CA, Brocker T. (2008) Constitutive crosspresentation of tissue antigens by dendritic cells controls CD8+ T cell tolerance in vivo. Immunity. 28(4):521-32.

Dottorandi di ricerca

Componente UO

Prof. Peggy Marconi

Dottorato di ricerca

Scienze biomediche e biotecnologiche

Coordinatore

Paolo Pinton

Sede

Università di Ferrara