Gulisano Massimo

Responsabile dell'U.O.

Cognome e Nome

Gulisano Massimo

Qualifica

PO

Dipartimento

Scienze del Farmaco e della Salute (DSFS)

Settore scientifico disciplinare

BIO/13 Biologia Applicata

E-mail

m.gulisano@unict.it

Telefono

+39 095 7384110

Personale strutturato

Cognome e Nome

Leanza Giampiero

Qualifica

PO

Dipartimento

Scienze del Farmaco e della Salute (DSFS)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Parenti Rosalba

Qualifica

PO

Dipartimento

Scienze Biomediche e Biotecnologiche (BIOMETEC)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Gulino Rosario

Qualifica

PA

Dipartimento

Scienze Biomediche e Biotecnologiche (BIOMETEC)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Zappalà Agata

Qualifica

PA

Dipartimento

Scienze Biomediche e Biotecnologiche (BIOMETEC)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Vicario Nunzio

Qualifica

RTDB

Dipartimento

Scienze Biomediche e Biotecnologiche (BIOMETEC)

Ente di appartenenza

Università degli Studi di Catania

Personale non strutturato

Cognome e Nome

Alberghina Cristiana

Qualifica

Ph.D student

Dipartimento

Scienze Biomediche e Biotecnologiche (BIOMETEC)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Ersilia Vinci

Qualifica

Assegnista

Dipartimento

Scienze del Farmaco e della Salute (DSFS)

Ente di appartenenza

Università degli Studi di Catania

Cognome e Nome

Costantino Angelita

Qualifica

Assegnista

Dipartimento

Scienze del Farmaco e della Salute (DSFS)

Ente di appartenenza

Università degli Studi di Catania

Linee di ricerca

1. Mechanisms underlying cognitive and/or sensorimotor deficits and their modeling in rodents 2. Activity dependent modulation of hippocampal neurogenesis and cognitive abilities 3. Mechanisms underlying spontaneous or drug/cell-mediated neuroplasticity in rodent models of motoneuron disease 4. Cell based repair strategies in animal models of CNS injury or degeneration 5. Intercellular communication and regenerative mechanisms in neuroinflammatory and neurodegenerative disorders 6. Role of toxic protein accumulation and aggregation in neurodegenerative disorders: Sigma 1 receptors as a target for disease modifying therapies in neurodegenerative disorders 7. Cellular and molecular biomarkers of brain tumors and potential therapeutic approaches: from drug screening to radiotherapy 8. Osteochondral stem cells and regenerative medicine using collagen/hydroxyapatite scaffolds: in vitro, in vivo and translational modelling 9. Epithelial Mesenchimal Transition (EMT) molecular mechanism in embryo development, neurobiology and cancer 10. Nutraceutical and drug screening strategies optimization in patient-derived organoid 3D models.

Tecnologie in uso dall'UO

  1. 1.
    Analysis of sensory-motor and cognitive behaviour
  2. 2.
    Stereotaxic surgery on rodents
  3. 3.
    2D and 3D cell cultures (including neurospheres and organoids)
  4. 4.
    Histo-and immunohistochemistry
  5. 5.
    Western blotting
  6. 6.
    Microscopy and confocal imaging
  7. 7.
    Unbiased stereology
  8. 8.
    Image analysis
  9. 9.
    In vivo live imaging technology (Preclinical Ultrasound photoacoustic and MicroPET/CT)

Strumentazione

Denominazione

Devices for stereotaxic microsurgery
Nikon Eclipse 80i light/fluorescence microscope
Olympus motorized light/fluorescence microscope
Leica anatomic pathology scanner Aperio imagescope
Reichert-Jung cryostat
Devices for rodent behaviour analyses
Multiskan Sky Microplate Spectrophotometer
Cell culture room
Leica DM IRB fluorescence microscope
Leica TCS SP8 confocal microscope
FUJIFILM VisualSonics Vevo LAZRX
Micro-PET-CT
Leica MZ FLIII fluorescence stereomicroscope

Struttura ove la strumentazione è allocata

(DSFS)
(DSFS)
(DSFS)
(DSFS)
(BIOMETEC)
(BIOMETEC)
(BIOMETEC)
(BIOMETEC)
(BIOMETEC)
BRIT (UNICT)
BRIT (UNICT)
BRIT (UNICT)
(DSFS)

Responsabile

Giampiero Leanza
Giampiero Leanza
Giampiero Leanza
Giampiero Leanza
Rosario Gulino
Rosario Gulino
Rosario Gulino
Rosalba Parenti
Rosalba Parenti
Rosalba Parenti
Rosalba Parenti
Rosalba Parenti
Massimo Gulisano

Pubblicazioni

  1. 1.
    D.W. Kasongo, G. de Leo, N. Vicario, G. Leanza*, G. Legname* Chronic alpha-synuclein accumulation in rat hippocampus induces Lewy Bodies formation and specific cognitive impairments ENeuro 7(3) (2020) (*Co-Corresponding Authors)
  2. 2.
    E. Giusto, M. Codrich, G. de Leo, V. Francardo, M. Coradazzi, R. Parenti, M. Gulisano, N. Vicario, R. Gulino, G. Leanza Compensatory changes in degenerating spinal motoneurons sustain functional sparing in the SOD1 G93A mouse model of amyotrophic lateral sclerosis. J. Comparative Neurology (2020) 528, 231-243.
  3. 3.
    Pisciotta, P; Costantino, A; Cammarata, FP; Torrisi, F; Calabrese, G; Marchese, V; Cirrone, GAP; Petringa, G; Forte, GI; Minafra, L; Bravatà, V; Gulisano, M; Scopelliti, F; Tommasino, F; Scifoni, E; Cuttone, G; Ippolito, M; Parenti, R; Russo, G (2020) "Evaluation of proton beam radiation-induced skin injury in a murine model using a clinical SOBP", PLoS ONE 15(5): e0233258. (doi.org/10.1371/journal.pone.0233258). Epub 2020 May 22
  4. 4.
    Caruso, G, Musso N, Grasso M, Costantino A, Lazzarino G, Tascedda F, Gulisano M, Lunte SM Caraci F (2020) "Microfluidics as a novel tool for biological and toxicological assays in drug discovery processes: focus on microchip electrophoresis", Micromachines (Special Issue “Lab-on-a-Chip Systems for Toxicology”) 2020, 11(6), 593 (doi.org/10.3390/mi11060593 (registering DOI)) Epub 2020 June 15
  5. 5.
    Torrisi F, Vicario N, Spitale FM, Cammarata FP, Minafra L, Salvatorelli L, Russo G, Cuttone G, Valable S, Gulino R, Magro G, Parenti R. The Role of Hypoxia and SRC Tyrosine Kinase in Glioblastoma Invasiveness and Radioresistance. Cancers (Basel). 2020 Oct 4;12(10):2860. doi: 10.3390/cancers12102860. PMID: 33020459; PMCID: PMC7599682.
  6. 6.
    Spitale FM, Vicario N, Rosa MD, Tibullo D, Vecchio M, Gulino R, Parenti R. Increased expression of connexin 43 in a mouse model of spinal motoneuronal loss. Aging (Albany NY). 2020 Jun 24;12(13):12598-12608. doi: 10.18632/aging.103561. Epub 2020 Jun 24. PMID: 32579130; PMCID: PMC7377853.
  7. 7.
    Vicario N, Turnaturi R, Spitale FM, Torrisi F, Zappalà A, Gulino R, Pasquinucci L, Chiechio S, Parenti C, Parenti R. Intercellular communication and ion channels in neuropathic pain chronicization. Inflamm Res. 2020 Sep;69(9):841-850. doi: 10.1007/s00011-020- 01363-9. Epub 2020 Jun 12. PMID: 32533221.
  8. 8.
    Torrisi F, Minafra L, Cammarata FP, Savoca G, Calvaruso M, Vicario N, Maccari L, Pérès EA, Özçelik H, Bernaudin M, Botta L, Russo G, Parenti R, Valable S. SRC Tyrosine Kinase Inhibitor and X-rays Combined Effect on Glioblastoma Cell Lines. Int J Mol Sci. 2020 May 30;21(11):3917. doi: 10.3390/ijms21113917. PMID: 32486205; PMCID: PMC7312922
  9. 9.
    N. Vicario, L. Pasquinucci, F.M. Spitale, S. Chiechio, R. Turnaturi, F. Caraci, D. Tibullo, R. Avola, R. Gulino, R. Parenti, C. Parenti. Simultaneous Activation of Mu and Delta Opioid Receptors Reduces Allodynia and Astrocytic Connexin 43 in an Animal Model of Neuropathic Pain. Molecular Neurobiology (2019) 56, 7338-7354.
  10. 10.
    J.D. Bernstock, L. Peruzzotti-Jametti, T. Leonardi, N. Vicario, D. Ye, Y.J. Lee, D. Maric, K.R. Johnson, Y. Mou, A. Van Den Bosch, M. Winterbone, G.K. Friedman, R.J.M. Franklin, J.M. Hallenbeck, S. Pluchino. SUMOylation promotes survival and integration of neural stem cell grafts in ischemic stroke. EBioMedicine (2019) 42, 214-224.

Dottorati di ricerca

Componente UO

Rosalba Parenti
Giampiero Leanza

Dottorato di ricerca

PhD program in Biotecnologie
PhD Program in Structural and Functional Genomics

Coordinatore

Prof. V. De Pinto
Prof. A. Mallamaci

Sede

UNICT
SISSA-ISAS Trieste