Responsabile dell'U.O.
Cognome e Nome
Vannini Candida
Qualifica
PA
Dipartimento
Biotechnology and Life Science (DBSV)
Settore scientifico disciplinare
BIO/04
candida.vannini@uninsubria.it
Telefono
0332 421418
Personale strutturato
Cognome e Nome
BRACALE MARCELLA
Qualifica
PO
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
MARSONI MILENA
Qualifica
Technician
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Personale non strutturato
Cognome e Nome
DOMINGO GUIDO
Qualifica
Post-doc
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
MANCINI ILARIA
Qualifica
Ph.D student
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
COSTANTINI ELENA
Qualifica
Ph.D student
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Linee di ricerca
design new strategies to screen germplasm for stress tolerance traitsediting) approaches to improve stress tolerance in crop species- deliver new genes to be introduced into classical breeding and new breeding (genome- obtain a global view of the molecular mechanisms related to plant stress response;Data from our research will permit to:stress biology.peptidomics) with physiological and functional genomics studies to identify key regulators in plantWe combine proteomic approaches (whole cell or subcellular analysis, phosphoproteomics,interaction between plants and beneficial or pathogenic microorganisms.which are interrelated and greatly affect plant productivity. We also aim to characterize theto environmental stresses. We are mainly interested to study temperature stresses and drought,Our biotechnological research focuses on analyzing physiological and molecular responses of plants
Tecnologie in uso dall'UO
- Protein extraction from whole cell or subcellular compartments
- Label and gel free proteomic and phosphoproteomic
- Peptides isolation and LC-MS identification
- Bioinformatic processing of proteomic data
- Metabolites detection and quantification by LC-MSM
- Molecular biology techniques (Real-Time PCR etc…)
- Plant cell cultures techniques
- Plant transformation procedures (Arabidopsis, tobacco, tomato)
Strumentazione
Denominazione
Thermofisher Linear ion-trap Mass spectometer
Thermofisher autosampler
Thermofisher HPLC
Real-Time PCR
Struttura ove la strumentazione è allocata
Proteomic Laboratory
Proteomic Laboratory
Laboratorio Microscopie Avanzate
Centro grandi attrezzature
Responsabile
Marsoni M.
Marsoni M.
Marsoni M.
Pubblicazioni
- Vannini C, Marsoni M, Scoccianti V, Ceccarini C, Domingo G, Bracale M, Crinelli R. Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination. Journal of Proteomics 192: 334-345.
- Domingo G, Villa F, Vannini C, Garuglieri E, Onelli E, Bracale M, Cappitelli F. Labelfree proteomic approach to study the non-lethal effects of silver nanoparticles on a gut bacterium. Front. Microbiol. 2019 10 (2709)
- Fiorilli V, Vannini C, Ortolani F, Garcia-Seco D, Chiapello M, Novero M, Domingo G, Terzi V, Morcia C, Bagnaresi P, Moulin L, Bracale M, Bonfante P. Omics approaches revealed how arbuscular mycorrhizal symbiosis enhances yield and resistance to leaf pathogen in wheat. Sci Rep 2018 8, 9625.
- Guarino MP, Altomare A, Barera S, et al. Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon. PLoS One. 2017 12(1):e0169481.
- Verrillo F, Badeck FW, Terzi V, Rizza F, Bernardo L, Di Maro A, Fares C, Zaldei A, Miglietta F, Moschella A, Bracale M, Vannini C. Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains. Crop and Pasture Science. 2017 68: 713-725.
- Parola S, Chiodaroli L, Orlandi V, Vannini C, Panno l. Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds. Functional Foods in Health and Disease. 2017 7: 773
- Garcia Seco D, Chiapello M, Bracale M, Pesce C, Bagnaresi P, Dubois E, Mulin L, Vannini C, Koebnik R. Transcriptome and proteome analysis reveal new insight into proximal and distal responses of wheat to foliar infection by Xanthomonas translucens. Sci Rep 2017 7: 10157.
- Sabetta W, Vannini C, Sgobba A, Marsoni M, Paradiso A, Ortolani F, Bracale M, Viggiano L, Blanco E, de Pinto MC. Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells. Plant Mol Biol. 2016 90(4-5):467-83.
- Vannini C, Carpentieri C, Salvioli A, Novero M, Marsoni M, Testa L, de Pinto MC, Amoresano A, Ortolani F, Bracale M, Bonfante. An interdomain network: the endobacterium of a mycorrhizal fungus promotes antioxidative responses in both fungal and plant hosts. New Phytol. 2016 211(1):265-75.
- Vannini C, Bracale M, Crinelli R, et al. Proteomic analysis of MG132-treated germinating pollen reveals expression signatures associated with proteasome inhibition. PLoS One. 2014 9(9):e108811
Dottorati di ricerca
Componente UO
Candida Vannini
Marcella Bracale
Dottorato di ricerca
Scienze della Vita e Biotecnologie
Scienze della Vita e Biotecnologie
Coordinatore
Silvia Sacchi
Silvia Sacchi
Sede
Insubria
Insubria