Responsabile dell'U.O.
Cognome e Nome
Vannini Candida
Qualifica
PA
Dipartimento
Biotechnology and Life Science (DBSV)
Settore scientifico disciplinare
BIO/04
candida.vannini@uninsubria.it
Telefono
0332 421418
Personale strutturato
Cognome e Nome
BRACALE MARCELLA
Qualifica
PO
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
MARSONI MILENA
Qualifica
Technician
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
DOMINGO GUIDO
Qualifica
Ricercatore
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Personale non strutturato
Cognome e Nome
DAVIDE ELEONORA
Qualifica
Ph.D student
Dipartimento
Biotechnology and Life Science (DBSV)
Ente di appartenenza
University of Insubria
Linee di ricerca
Our research group primarily concentrates on the following areas:
1. Understanding the physiology and molecular biology of plant responses to challenging environments, such as high temperatures and drought.
2. Analysis of the interaction between agronomically significant plants and beneficial soil microorganisms associated with the roots, with a specific focus on mycorrhizal symbiosis.
3. Extraction and purification of bioactive peptides and proteins of medical and industrial interest from plant-based sources.
4. Evaluation of the biostimulant and bioprotective properties of compounds of plant and animal origin, as well as waste-derived molecules, on crops.
5. Production of recombinant proteins in plant.
These studies involve extensive physiological and molecular analyses, with a particular emphasis on proteomics and metabolomics.
Tecnologie in uso dall'UO
- 1.Protein and peptide extraction from whole cell or subcellular compartments
- 2.Label and gel free proteomic and phosphoproteomic
- 3.LC-MS identification
- 4.Bioinformatic processing of proteomic data
- 5.Metabolites detection and quantification by LC-MSM
- 6.Plant cell cultures techniques
- 7.Plant transformation procedures (Arabidopsis, tobacco, tomato)
Strumentazione
Denominazione
Thermofisher Linear ion-trap Mass spectometer
Thermofisher autosampler
Thermofisher HPLC
Struttura ove la strumentazione è allocata
Proteomic Laboratory
Proteomic Laboratory
Laboratorio Microscopie Avanzate
Responsabile
Marsoni M.
Marsoni M.
Marsoni M.
Pubblicazioni
- 1.Vannini C, Marsoni M, Scoccianti V, Ceccarini C, Domingo G, Bracale M, Crinelli R. Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination. Journal of Proteomics 192: 334-345.
- 2.Domingo G, Villa F, Vannini C, Garuglieri E, Onelli E, Bracale M, Cappitelli F. Labelfree proteomic approach to study the non-lethal effects of silver nanoparticles on a gut bacterium. Front. Microbiol. 2019 10 (2709)
- 3.Fiorilli V, Vannini C, Ortolani F, Garcia-Seco D, Chiapello M, Novero M, Domingo G, Terzi V, Morcia C, Bagnaresi P, Moulin L, Bracale M, Bonfante P. Omics approaches revealed how arbuscular mycorrhizal symbiosis enhances yield and resistance to leaf pathogen in wheat. Sci Rep 2018 8, 9625.
- 4.Guarino MP, Altomare A, Barera S, et al. Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon. PLoS One. 2017 12(1):e0169481.
- 5.Verrillo F, Badeck FW, Terzi V, Rizza F, Bernardo L, Di Maro A, Fares C, Zaldei A, Miglietta F, Moschella A, Bracale M, Vannini C. Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains. Crop and Pasture Science. 2017 68: 713-725.
- 6.Parola S, Chiodaroli L, Orlandi V, Vannini C, Panno l. Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds. Functional Foods in Health and Disease. 2017 7: 773
- 7.Garcia Seco D, Chiapello M, Bracale M, Pesce C, Bagnaresi P, Dubois E, Mulin L, Vannini C, Koebnik R. Transcriptome and proteome analysis reveal new insight into proximal and distal responses of wheat to foliar infection by Xanthomonas translucens. Sci Rep 2017 7: 10157.
- 8.Sabetta W, Vannini C, Sgobba A, Marsoni M, Paradiso A, Ortolani F, Bracale M, Viggiano L, Blanco E, de Pinto MC. Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells. Plant Mol Biol. 2016 90(4-5):467-83.
- 9.Vannini C, Carpentieri C, Salvioli A, Novero M, Marsoni M, Testa L, de Pinto MC, Amoresano A, Ortolani F, Bracale M, Bonfante. An interdomain network: the endobacterium of a mycorrhizal fungus promotes antioxidative responses in both fungal and plant hosts. New Phytol. 2016 211(1):265-75.
- 10.Vannini C, Bracale M, Crinelli R, et al. Proteomic analysis of MG132-treated germinating pollen reveals expression signatures associated with proteasome inhibition. PLoS One. 2014 9(9):e108811
Dottorati di ricerca
Componente UO
Candida Vannini
Marcella Bracale
Dottorato di ricerca
Scienze della Vita e Biotecnologie
Scienze della Vita e Biotecnologie
Coordinatore
Silvia Sacchi
Silvia Sacchi
Sede
Insubria
Insubria