Vannini Candida


Responsabile dell'U.O.

Cognome e Nome

Vannini Candida

Qualifica

PA

Dipartimento

Biotechnology and Life Science (DBSV)

Settore scientifico disciplinare

BIO/04

E-mail

candida.vannini@uninsubria.it

Telefono

0332 421418

Personale strutturato

Cognome e Nome

BRACALE MARCELLA

Qualifica

PO

Dipartimento

Biotechnology and Life Science (DBSV)

Ente di appartenenza

University of Insubria

Cognome e Nome

MARSONI MILENA

Qualifica

Technician

Dipartimento

Biotechnology and Life Science (DBSV)

Ente di appartenenza

University of Insubria

Personale non strutturato

Cognome e Nome

DOMINGO GUIDO

Qualifica

Post-doc

Dipartimento

Biotechnology and Life Science (DBSV)

Ente di appartenenza

University of Insubria

Cognome e Nome

MANCINI ILARIA

Qualifica

Ph.D student

Dipartimento

Biotechnology and Life Science (DBSV)

Ente di appartenenza

University of Insubria

Cognome e Nome

COSTANTINI ELENA

Qualifica

Ph.D student

Dipartimento

Biotechnology and Life Science (DBSV)

Ente di appartenenza

University of Insubria

Linee di ricerca

design new strategies to screen germplasm for stress tolerance traitsediting) approaches to improve stress tolerance in crop species- deliver new genes to be introduced into classical breeding and new breeding (genome- obtain a global view of the molecular mechanisms related to plant stress response;Data from our research will permit to:stress biology.peptidomics) with physiological and functional genomics studies to identify key regulators in plantWe combine proteomic approaches (whole cell or subcellular analysis, phosphoproteomics,interaction between plants and beneficial or pathogenic microorganisms.which are interrelated and greatly affect plant productivity. We also aim to characterize theto environmental stresses. We are mainly interested to study temperature stresses and drought,Our biotechnological research focuses on analyzing physiological and molecular responses of plants

Tecnologie in uso dall'UO

  1. 1.
    Protein extraction from whole cell or subcellular compartments
  2. 2.
    Label and gel free proteomic and phosphoproteomic
  3. 3.
    Peptides isolation and LC-MS identification
  4. 4.
    Bioinformatic processing of proteomic data
  5. 5.
    Metabolites detection and quantification by LC-MSM
  6. 6.
    Molecular biology techniques (Real-Time PCR etc…)
  7. 7.
    Plant cell cultures techniques
  8. 8.
    Plant transformation procedures (Arabidopsis, tobacco, tomato)

Strumentazione

Denominazione

Thermofisher Linear ion-trap Mass spectometer
Thermofisher autosampler
Thermofisher HPLC
Real-Time PCR

Struttura ove la strumentazione è allocata

Proteomic Laboratory
Proteomic Laboratory
Laboratorio Microscopie Avanzate
Centro grandi attrezzature

Responsabile

Marsoni M.
Marsoni M.
Marsoni M.

Pubblicazioni

  1. 1.
    Vannini C, Marsoni M, Scoccianti V, Ceccarini C, Domingo G, Bracale M, Crinelli R. Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination. Journal of Proteomics 192: 334-345.
  2. 2.
    Domingo G, Villa F, Vannini C, Garuglieri E, Onelli E, Bracale M, Cappitelli F. Labelfree proteomic approach to study the non-lethal effects of silver nanoparticles on a gut bacterium. Front. Microbiol. 2019 10 (2709)
  3. 3.
    Fiorilli V, Vannini C, Ortolani F, Garcia-Seco D, Chiapello M, Novero M, Domingo G, Terzi V, Morcia C, Bagnaresi P, Moulin L, Bracale M, Bonfante P. Omics approaches revealed how arbuscular mycorrhizal symbiosis enhances yield and resistance to leaf pathogen in wheat. Sci Rep 2018 8, 9625.
  4. 4.
    Guarino MP, Altomare A, Barera S, et al. Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon. PLoS One. 2017 12(1):e0169481.
  5. 5.
    Verrillo F, Badeck FW, Terzi V, Rizza F, Bernardo L, Di Maro A, Fares C, Zaldei A, Miglietta F, Moschella A, Bracale M, Vannini C. Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains. Crop and Pasture Science. 2017 68: 713-725.
  6. 6.
    Parola S, Chiodaroli L, Orlandi V, Vannini C, Panno l. Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds. Functional Foods in Health and Disease. 2017 7: 773
  7. 7.
    Garcia Seco D, Chiapello M, Bracale M, Pesce C, Bagnaresi P, Dubois E, Mulin L, Vannini C, Koebnik R. Transcriptome and proteome analysis reveal new insight into proximal and distal responses of wheat to foliar infection by Xanthomonas translucens. Sci Rep 2017 7: 10157.
  8. 8.
    Sabetta W, Vannini C, Sgobba A, Marsoni M, Paradiso A, Ortolani F, Bracale M, Viggiano L, Blanco E, de Pinto MC. Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells. Plant Mol Biol. 2016 90(4-5):467-83.
  9. 9.
    Vannini C, Carpentieri C, Salvioli A, Novero M, Marsoni M, Testa L, de Pinto MC, Amoresano A, Ortolani F, Bracale M, Bonfante. An interdomain network: the endobacterium of a mycorrhizal fungus promotes antioxidative responses in both fungal and plant hosts. New Phytol. 2016 211(1):265-75.
  10. 10.
    Vannini C, Bracale M, Crinelli R, et al. Proteomic analysis of MG132-treated germinating pollen reveals expression signatures associated with proteasome inhibition. PLoS One. 2014 9(9):e108811

Dottorati di ricerca

Componente UO

Candida Vannini
Marcella Bracale

Dottorato di ricerca

Scienze della Vita e Biotecnologie
Scienze della Vita e Biotecnologie

Coordinatore

Silvia Sacchi
Silvia Sacchi

Sede

Insubria
Insubria