La Starza Roberta


Responsabile dell'U.O.

Cognome e Nome

La Starza Roberta

Qualifica

Ricercatore

Dipartimento

Medicine and Surgery

Settore scientifico disciplinare

MED/15 - MALATTIE DEL SANGUE

E-mail

roberta.lastarza@unipg.it

Telefono

075 5783455

Personale strutturato

Cognome e Nome

Martelli Maria Paola

Qualifica

PA

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Pierini Antonio

Qualifica

PA

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Caterina Matteucci

Qualifica

Technician

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Personale non strutturato

Cognome e Nome

Bardelli Valentina

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Lema Fernandez Anair Graciela

Qualifica

RTDA

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Nardelli Carlotta

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Milano Francesca

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Ranieri Roberta

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Pierangeli Sara

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Mancusi Antonella

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Cipriani Sabrina

Qualifica

Post-doc

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Cognome e Nome

Servoli Francesca

Qualifica

Ph.D student

Dipartimento

Medicine and Surgery

Ente di appartenenza

University of Perugia

Linee di ricerca

The Laboratory implements the most innovative diagnostic procedures for continuous improvement in the characterization of oncohematologic diseases for which an integrated clinical, hematologic, morphologic, hematopathologic, immunophenotypic, molecular-cytogenetics, next generation sequencing tools and epigenetic studies are used to pinpoint prognostic and predictive biomarkers. The laboratory is JACIE accredited for cell manipulation, AIFA and for performing diagnostic tests required for phase I clinical trials, and GenomicQualityAssessment, and it is a reference Center of the GIMEMA National Network for the diagnosis and treatment of acute leukemia. The main fields of research are represented by the genomic and epigenetic characterization of Acute Lymphoblastic and Myeloblastic Leukemias, Myelodysplastic Syndromes, Chronic Myelo- and Lympho- Proliferative Neoplasms, Hodgkin and Non-Hodgkin Lymphomas, and Hairy Cell Leukemia. We have also developed a line of research in post-transplant immunologic reconstitution and cell therapy, such as immunotherapy and CAR-T. The laboratory is also a reference Center for Congenital and Acquired Immunodeficiencies and Germline Predisposing Conditions, for which enables a precise molecular diagnosis through the application of custom target NGS panels. Translational research is linked in real time to diagnostics, in a continuous osmosis between the Departments of Hematology/DH and Laboratories. Therefore, the University component is relevant and the contribution to diagnosis decisive. The importance of a set of multidisciplinary laboratories that interface and operate in an integrated constitutes the added value for the real-time initiation of rapid and accurate diagnostic pathways, in each individual patient, in order to implement: 1) precise prognostic stratification of the patient; 2) make the best treatment choice in relation to the biological characteristics of the disease; 3) schedule continuous monitoring.

Tecnologie in uso dall'UO

  1. 1.
    Cytomorphology, flow cytometry and hemopathology
  2. 2.
    Target Next Generation Sequencing, DNA Single Cell Sequencing (Tapestri)
  3. 3.
    RNAseq
  4. 4.
    mERRBS
  5. 5.
    Cell transduction with viral vectors
  6. 6.
    CRISPR/CASP9
  7. 7.
    Gene cloning
  8. 8.
    Digital PCR and Real time PCR
  9. 9.
    Conventional Cytogenetics, DNA microarray, RNA microarray, FICTION
  10. 10.
    Selection, grow and purification of DNA clones

Strumentazione

Denominazione

ASI system (SPOTScan/METAScan/GenASIs/FISHview/BANDview, MICROSCOPIO OLYMPUS BX61)
DILUITORE HAMILTON MEDICAL AG, MICROLAB STARLET
EliSpot reader No. ELR08IFl2909359: AID iSpot con training online, monitor, lettore Elispot e chemioluminescenza
MultiRad225 Irradiator
Mission Bio Tapestry Platform for Single-cell Sequencing
MiSeq Illumina (3 instruments)
Flow Cytometer, BD Fortessa Fortessa
STEMvision Automated CFU Counting

Struttura ove la strumentazione è allocata

laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology
laboratory of integrated diagnostics of blood diseases, Hematology

Responsabile

Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza
Dr. Roberta La Starza

Pubblicazioni

  1. 1.
    Almeida A, T'Sas S, Pagliaro L, Fijalkowski I, Sleeckx W, Van Steenberge H, Zamponi R, Lintermans B, Van Loocke W, Palhais B, Reekmans A, Bardelli V, Demoen L, Reunes L, Deforce D, Van Nieuwerburgh F, Kentsis A, Ntziachristos P, Van Roy N, De Moerloose B, Mecucci C, La Starza R, Roti G, Goossens S, Van Vlierberghe P, Pieters T. Myb overexpression synergizes with the loss of Pten and is a dependency factor and therapeutic target in T-cell lymphoblastic leukemia. Hemasphere. 2024 Mar 10;8(3):e51. doi: 10.1002/hem3.51. PMID: 38463444; PMCID: PMC10924755.
  2. 2.
    Pierini V, Bardelli V, Giglio F, Arniani S, Matteucci C, Pellanera F, Quintini M, Crescenzi B, Bruno A, Sabattini E, Falcinelli E, Ruggeri L, Ronchi P, Ponzoni M, Ciceri F, Mecucci C, La Starza R. A Novel t(5;7)(q31;q21)/CDK6::IL3 in Immature T-cell Acute Lymphoblastic Leukemia With IL3 Expression and Eosinophilia. Hemasphere. 2022 Oct 19;6(11):e795. doi: 10.1097/HS9.0000000000000795. PMID: 36285071; PMCID: PMC9584195.
  3. 3.
    Arniani S, Pierini V, Pellanera F, Matteucci C, Di Giacomo D, Bardelli V, Quintini M, Mavridou E, Lema Fernandez AG, Nardelli C, Moretti M, Gorello P, Crescenzi B, Romoli S, Beacci D, Cerrano M, Fracchiolla N, Sica S, Forghieri F, Giglio F, Dargenio M, Elia L, La Starza R, Mecucci C. Chromothripsis is a frequent event and underlies typical genetic changes in early T-cell precursor lymphoblastic leukemia in adults. Leukemia. 2022 Nov;36(11):2577-2585. doi: 10.1038/s41375-022-01671-5. Epub 2022 Aug 16. PMID: 35974102; PMCID: PMC9613476.
  4. 4.
    Montanaro A, Kitara S, Cerretani E, Marchesini M, Rompietti C, Pagliaro L, Gherli A, Su A, Minchillo ML, Caputi M, Fioretzaki R, Lorusso B, Ross L, Alexe G, Masselli E, Marozzi M, Rizzi FMA, La Starza R, Mecucci C, Xiong Y, Jin J, Falco A, Knoechel B, Aversa F, Candini O, Quaini F, Sportoletti P, Stegmaier K, Roti G. Identification of an Epi-metabolic dependency on EHMT2/G9a in T-cell acute lymphoblastic leukemia. Cell Death Dis. 2022 Jun 17;13(6):551. doi: 10.1038/s41419-022-05002-5. PMID: 35710782; PMCID: PMC9203761.
  5. 5.
    Cerrano M, Bonifacio M, Olivi M, Curti A, Malagola M, Dargenio M, Scattolin AM, Papayannidis C, Forghieri F, Gurrieri C, Tanasi I, Zappasodi P, La Starza R, Fracchiolla NS, Chiusolo P, Giaccone L, Del Principe MI, Giglio F, Defina M, Favre C, Rizzari C, Castella B, Pizzolo G, Ferrara F, Chiaretti S, Foà R. Daratumumab with or without chemotherapy in relapsed and refractory acute lymphoblastic leukemia. A retrospective observational Campus ALL study. Haematologica. 2022 Apr 1;107(4):996-999. doi: 10.3324/haematol.2021.279851. PMID: 35021604; PMCID: PMC8968887.
  6. 6.
    Di Giacomo D, La Starza R, Gorello P, Pellanera F, Kalender Atak Z, De Keersmaecker K, Pierini V, Harrison CJ, Arniani S, Moretti M, Testoni N, De Santis G, Roti G, Matteucci C, Bassan R, Vandenberghe P, Aerts S, Cools J, Bornhauser B, Bourquin JP, Piazza R, Mecucci C. 14q32 rearrangements deregulating BCL11B mark a distinct subgroup of T-lymphoid and myeloid immature acute leukemia. Blood. 2021 Sep 2;138(9):773-784. doi: 10.1182/blood.2020010510. PMID: 33876209; PMCID: PMC8513670.
  7. 7.
    Bardelli V, Arniani S, Pierini V, Pierini T, Di Giacomo D, Gorello P, Moretti M, Pellanera F, Elia L, Vitale A, Storlazzi CT, Tolomeo D, Mastrodicasa E, Caniglia M, Chiaretti S, Ruggeri L, Roti G, Schwab C, Harrison CJ, Almeida A, Pieters T, Van Vlierberghe P, Mecucci C, La Starza R. MYB rearrangements and over-expression in T-cell acute lymphoblastic leukemia. Genes Chromosomes Cancer. 2021 Jul;60(7):482-488. doi: 10.1002/gcc.22943. Epub 2021 Mar 8. PMID: 33611795.
  8. 8.
    Guardalupi F, Sorrentino C, Corradi G, Giancola R, Baldoni S, Ulbar F, Fabi B, Andres Ejarque R, Timms J, Restuccia F, Santarone S, Accorsi P, Sportoletti P, De Falco F, Rosati E, Carotti A, Falzetti F, Velardi A, Martelli MF, Kordasti S, Pierini A, Ruggeri L, Di Ianni M. A pro-inflammatory environment in bone marrow of Treg transplanted patients matches with graft-versus-leukemia effect. Leukemia. 2023 Jul;37(7):1572-1575. doi: 10.1038/s41375-023-01932-x. Epub 2023 Jun 7. PMID: 37286785; PMCID: PMC10317833.
  9. 9.
    Pierini A, Ruggeri L, Carotti A, Falzetti F, Saldi S, Terenzi A, Zucchetti C, Ingrosso G, Zei T, Iacucci Ostini R, Piccinelli S, Bonato S, Tricarico S, Mancusi A, Ciardelli S, Limongello R, Merluzzi M, Di Ianni M, Tognellini R, Minelli O, Mecucci C, Martelli MP, Falini B, Martelli MF, Aristei C, Velardi A. Haploidentical age-adapted myeloablative transplant and regulatory and effector T cells for acute myeloid leukemia. Blood Adv. 2021 Mar 9;5(5):1199-1208. doi: 10.1182/bloodadvances.2020003739. PMID: 33646302; PMCID: PMC7948281.
  10. 10.
    La Starza R, Cambò B, Pierini A, Bornhauser B, Montanaro A, Bourquin JP, Mecucci C, Roti G. Venetoclax and Bortezomib in Relapsed/Refractory Early T-Cell Precursor Acute Lymphoblastic Leukemia. JCO Precis Oncol. 2019 Sep 20;3:PO.19.00172. doi: 10.1200/PO.19.00172. PMID: 32923866; PMCID: PMC7448796.

Dottorati di ricerca

Componente UO

La Starza Roberta
Martelli Maria Paol
Pierini Antonio

Dottorato di ricerca

Clinical and molecular medicine
Clinical and molecular medicine
Clinical and molecular medicine

Coordinatore

Prof. Maria Paola Martelli
Prof. Maria Paola Martelli
Prof. Maria Paola Martelli

Sede

Perugia
Perugia
Perugia