Masci Stefania Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Masci Stefania
Qualifica PROFESSORE ASSOCIATO
Dipartimento Agricultural and Forestry Science (DAFNE)
Settore Scientifico Disciplinare AGR/07
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tel +390761357255;+393288641241

PERSONALE STRUTTURATO

Cognome e Nome Lafiandra Domenico
Qualifica PO
Dipartimento DAFNE
Ente di appartenenza Tuscia University
Cognome e Nome Sestili Francesco
Qualifica RU
Dipartimento DAFNE
Ente di appartenenza Tuscia University

PERSONALE NON STRUTTURATO

Cognome e Nome Botticella Ermelinda
Qualifica Post doc fellow
Dipartimento DAFNE
Ente di appartenenza Tuscia University
Cognome e Nome Lupi Roberta
Qualifica Post doc fellow
Dipartimento DAFNE
Ente di appartenenza Tuscia University
Cognome e Nome Crmerlengo Francesco
Qualifica PhD student
Dipartimento DAFNE
Ente di appartenenza Tuscia University

LINEE DI RICERCA

The research is directed at understanding the genetic and molecular bases of wheat technological and nutritional properties, and at the manipulation of wheat kernel composition in order to obtain new wheat genotypes with improved properties.

In particular, the research is focused on wheat kernel proteins related to dough technological quality (gluten proteins), to adverse reactions to wheat (such as celiac disease, wheat sensitivity, wheat allergies), and on the manipulation of wheat starch composition, by altering the activity of genes related to starch metabolism


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Chemical mutagenesis
  • Durum wheat TILLING platform
  • Gel-based proteomics
  • Immunoassays by using sera of allergic patients and specific antibodies
  • Rapid Visco Analyser
  • Gene silencing by RNAi
  • Real Time PCR
  • SNP genotyping

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
LightScanner (Idaho) DAFNE Sestili/Lafiandra
Gel-based proteomics (2D electrophoresis) DAFNE Masci
DIGE system CGA Masci
HPLC DAFNE Masci/Lafiandra
LightCycler 480 (Roche) CGA Simeone

PUBBLICAZIONI

Giuliani M.M, Palermo C., De Santis M.A., Mentana A., Pompa M., Giuzio L., Masci S., Centonze D., Flagella Z., 2015. Differential expression of durum wheat gluten proteome under water stress during grain filling. Journal of Agricultural and Food Chemistry 63(29):6501–6512

Masci S., Laino P., Janni M., Botticella E., Di Carli M., Benvenuto E., Danieli P.P., Lilley K.S., Lafiandra D., D’Ovidio R., 2015. Analysis of quality-related parameters in mature kernels of PGIP transgenic bread wheat infected with Fusarium graminearum. Journal of Agricultural and Food Chemistry 63(15):3962-3969

Rakszegi, M., Kisgyörgy, B.N., Kiss, T., Sestili, F., Láng, L., Lafiandra, D., Bedö, Z. Development and characterization of high-amylose wheat lines, 2015. Starch/Staerke, 67 (3-4), pp. 247-254

Sestili, F., Palombieri, S., Botticella, E., Mantovani, P., Bovina, R., Lafiandra, D. TILLING mutants of durum wheat result in a high amylose phenotype and provide information on alternative splicing mechanisms, 2015. Plant Science, 233:127-133

Egidi E., Sestili F., Janni M., D’Ovidio R., Lafiandra D., Ceriotti A., Vensel W.H., Kasarda D.D., Masci S., 2014. An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm. BMC Plant Biology BMC Plant Biology, 14:64

Lupi R., Masci S., Rogniaux H., Tranquet O., Brossard C., Lafiandra D., Moneret-Vautrin A.D., Denery-Papini S., Larré C., 2014. Assessment of the allergenicity of soluble fractions from GM and commercial genotypes of wheats. Journal of Cereal Science 60:179-186

Sparla F., Falini G., Botticella E., Pirone C., Talamè V., Bovina R., Salvi S., Tuberosa R., Sestili F., Trost P., 2014. New starch phenotypes produced by TILLING in barley. PLoS ONE, 9(10):e107779.

Wang, X., Feng, B., Xu, Z., Sestili, F., Zhao, G., Xiang, C., Lafiandra, D., Wang, T. Identification and characterization of granule bound starch synthase I (GBSSI) gene of tartary buckwheat (Fagopyrum tataricum Gaertn.), 2014. Gene, 534:229-235.

Lupi R., Denery-Papini S., Rogniaux H., Lafiandra D., Rizzi C., De Carli M., Moneret-Vautrin A.D., Masci S., Larré C., 2013. How much does transgenesis affect wheat allergenicity? Assessment in two GM lines over-expressing endogenous genes. Journal of Proteomics 80:281-291

Sestili F., Paoletti F., Botticella E., Masci S., Saletti R., Muccilli V., Lafiandra D., 2013. Comparative proteomic analysis of kernel proteins of two high amylose transgenic durum wheat lines obtained by biolistic and Agrobacterium-mediated transformations. Journal of Cereal Science 58:15-22


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Masci Stefania Plant Biotechnology (up to the XXVIII Cycle) Masci Stefania Viterbo
Masci Stefania Plant and Animal Science (starting from the XXIX cycle)

Lafiandra Domenico (coordinator)

Masci Stefania (vice-coordinator)
Viterbo
Lafiandra Domenico Plant and Animal Science (starting from the XXIX cycle)

Lafiandra Domenico (coordinator)

Masci Stefania (vice-coordinator)
Viterbo
Sestili Plant and Animal Science (starting from the XXIX cycle)

Lafiandra Domenico (coordinator)

Masci Stefania (vice-coordinator)
Viterbo



CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10