Pagnotta Mario-Augusto Stampa

Cognome e Nome Pagnotta Mario-Augusto
Qualifica PA
Scienze Agrarie e Forestali (DAFNE)
Settore Scientifico Disciplinare AGR/07 (Genetica Agraria)
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Tel 0761357423


Cognome e Nome De Pace Ciro
Qualifica PO
Scienze Agrarie e Forestali (DAFNE)
Ente di appartenenza
Università della Tuscia
Cognome e Nome Vittori Doriano
Qualifica Tecnico
Scienze Agrarie e Forestali (DAFNE)
Ente di appartenenza
Università della Tuscia


Cognome e Nome
Mondini Linda
Scienze Agrarie e Forestali (DAFNE)
Ente di appartenenza
Università della Tuscia


1) Development of biometrical methods to study the genetic relationships and to estimate heritability in germplasm accessions of wheat, faba-bean, Jerusalem artichoke, artichoke, hazelnut and chestnut.

2) Identification of polymorphic biochemical (isoenzymes and storage proteins) and molecular genetic markers (RAPD, AFLP, RFLP, SCAR, SSR, SNP, Microarray, NGS) to (i) assess the genetic variation in natural populations of different domesticated plant species (i.e. forage legumes, Triticum spp., Caper, Poplar, Orchids, Artichoke, Pistachio, Chestnut and Hazelnut), (ii) transfer genes from wild to cultivated species (i.e. D. villosum – wheats) for enhancing resistance to biotic stresses (powdery mildew and rusts), and (iii) fingerprinting and varietal identification.

3) Construction of genetic maps and genes localization; in particular genes involved in the resistance to water stresses by identifying some allelic variants conferring tolerance. Analyzing the linkage disequilibrium, the Hitchhiking effects and the QTLs involved in tolerance to abiotic stresses.

4) Designing and application of an effective selection method of new AABB lines of durum wheat by hybridization between anphiploidi AABBVV and hexaploid wheats AABBDD.

5) Microarray monitoring of gene expression during the synthesis and accumulation of carbohydrate in the tubers of Helianthus tuberosus clones displaying high production of biomass in order to identify those suitable for efficient conversion into bioethanol.

6) Development of indicators to quantify the biological diversity and geographical spread of invasive species of forest pathogens and to make predictions on the adaptive capacity of cultivated species to environmental extremes induced by climate changes.

7) Assessment of problems connected with the biodiversity preservation and evaluation of the relationships between genetic variability distribution and some climatic and micro-climatic conditions at the origin sites, utilizing an eco-genetics approach.

8) Quantification and phylogenetic analysis of the level of dispersion of retrotransposons in genomes of different species within the Triticum and Aegilops genera.


  • Molecular marker (RFLP, SSR, STS, EST) analyses (agarose and acrylamide)
  • High-Resolution Melting (HRM))
  • Genetic mapping
  • Quantitative real-time RT-PCR


Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Pagnotta Mario-A
Liquid Handler Biomek
Pagnotta Mario-A


Olango Temesgen Magule, Bizuayehu Tesfaye, Pagnotta Mario Augusto, Pè Mario Enrico, Catellani Marcello. 2015. Development of SSR markers and genetic diversity analysis in enset (Ensete ventricosum (Welw.) Cheesman), an orphan food security crop of Southern Ethiopia. BMC Genetics 16:98. DOI 10.1186/s12863-015-0250-8

Mondini Linda, Nachit Miloudi Mikael, Pagnotta M.A. 2015. Allelic variants in durum wheat (Triticum turgidum L. var. durum) DREB genes conferring tolerance to abiotic stresses. Molecular Genetics and Genomics 290: 531-544. doi: 10.1007/s00438-014-0933-2

Kole C, Muthamilarasan M, Henry R, Edwards D, Sharma R, Abberton M, Batley J, Bentley A, Blakeney M, Bryant J, Cai H, Cakir M, Cseke LJ, Cockram J, de Oliveira AC, De Pace C, Dempewolf H, Ellison S, Gepts P, Greenland A, Hall A, Hori K, Hughes S, Humphreys MW, Iorizzo M, Ismail AM, Marshall A, Mayes S, Nguyen HT, Ogbonnaya FC, Ortiz R, Paterson AH, Simon PW, Tohme J, Tuberosa R, Valliyodan B, Varshney RK, Wullschleger SD, Yano M and Prasad M. 2015. Application of genomics-assisted breeding for generation of climate resilient crops: progress and prospects. Front. Plant Sci. 6:563. 11 August 2015 |

Abberton M., Batley J., Bentley A., Bryant J., Cai H., Cockram J., Costa de Oliveira A., Cseke L.J., Dempewolf H., De Pace C., Edwards D., Gepts P., Greenland A., Hall A.E., Henry R., Hori K., Howe G.T., Hughes S., Humphreys M., Lightfoot D., Marshall A., Mayes S., Nguyen H.T., Ogbonnaya F.C., Ortiz R., Paterson A.H., Tuberosa R., Valliyodan B., Varshney R.K. and Yano M. 2015, Global agricultural intensification during climate change: a role for genomics. Plant Biotechnol. J., pp. 1-4. doi: 10.1111/pbi.12467

Mondini Linda, Grausgruber Heinrich, Pagnotta M.A.  2015. Evaluation of European emmer wheat germplasm for agro-morphological, grain quality traits and molecular traits. Genetic Resources and Crop Evolution 61(1): 69-87. Doi 10.1007/s10722-013-0016-y

Santini A., L. Ghelardini, C. De Pace, M. L. Desprez-Loustau, P. Capretti, A. Chandelier, T. Cech, D. Chira, S. Diamandis, T. Gaitniekis, J. Hantula, O. Holdenrieder, L. Jankovsky, T. Jung, D. Jurc, T. Kirisits, A. Kunca, V. Lygis, M. Malecka, B. Marcais, S. Schmitz, J. Schumacher, H. Solheim, A. Solla, I. Szabò, P. Tsopelas, A. Vannini, A. M. Vettraino, J. Webber, S. Woodward, J. Stenlid, 2013. Biogeographical patterns and determinants of invasion by forest pathogens in Europe. New Phytologist 197(1): 238-250.

Ciancolini A., Rey NA, Pagnotta MA, Crinò P. 2012. Characterization of Italian spring globe artichoke germplasm: morphological and molecular profiles. Euphytica 186(2): 433-443. (DOI: 10.1007/s10681-012-0634-z)

Gennaro Andrea, Forte Paola, Panichi Daniela, Lafiandra Domenico, Pagnotta Mario Augusto, D’Egidio Maria Grazia, Ceoloni Carla. 2012. Stacking small segments of the 1D chromosome of bread wheat containing major gluten quality genes into durum wheat: transfer strategy and breeding prospects. Molecular Breeding 30(1): 149-167. (DOI 10.1007/s11032-011-9606-6).

De Pace C., M. Pasquini, P. Vaccino, M. Bizzarri, F. Nocente, M. Corbellini, M. E. Caceres, P. G. Cionini, D. Vittori, and G. Vida, 2011. Deployment of either a whole or dissected wild nuclear genome into the wheat gene pool meets the breeding challenges posed by the sustainable farming systems. Plant Genetic Resources, 9: 352-356.

Pagnotta M.A., Annicchiarico P., Farina A., Proietti S. 2011. Characterizing the molecular and morphophysiological diversity of Italian red clover. Euphytica, 179: 393-404. ISSN: 0014-2336, doi: 10.1007/s10681-010-0333-6



Componente U.O. Dottorato di Ricerca Coordinatore Sede
Pagnotta Mario A
Ecosistemi e sistemi produttivi
Sorrentino Alessandro
Università della Tuscia


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