Prat Maria Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Prat Maria
Qualifica Full Professor
Facoltà Medicina, Chirurgia e Scienze della salute
Dipartimento Scienze Mediche, Università del Piemonte Orientale
Settore Scientifico Disciplinare BIO/17
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PERSONALE STRUTTURATO

Cognome e Nome Rimondini Lia
Qualifica Associate Professor
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Follenzi Antonia
Qualifica Assistant Professor
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
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PERSONALE NON STRUTTURATO

Cognome e Nome Pietronave Stefano
Qualifica Senior Research Fellow
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Iafisco Michele
Qualifica Senior Research Fellow
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Merlin Simone
Qualifica Research Fellow
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Varoni Elena
Qualifica Ph.D. student
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Ranaldo Gabriella
Qualifica Ph.D. student
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Zamperone Andrea
Qualifica Ph.D. student
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
Cognome e Nome Cannizzo Elvira Stefania
Qualifica Ph.D. student
Dipartimento Scienze Mediche
Ente di appartenenza Universita’ del Piemonte Orientale
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LINEE DI RICERCA

The major interest of the R.U. is regenerative medicine and tissue engineering, in their fields of cell therapy, gene therapy and biomaterials with the goal to find out the best microenvironment conditions to produce cells with the desired phenotype starting from adult stem or progenitor cells.
Different conditions (celle culture on termosensitive polymeric substrates, which allow the production of cell sheets, electric stimulation in a bioreactor – joint project with the Politecnico di Milano-, addition of growth factors or molecules, such as monoclonal antibodies, which mimick their activity) to induce progenitor cells isolated from human auriculae in cardiosurgery or adult stem cells isolated from human or murine adipose tissue towards the cardiomyocytic phenotype.
We are also setting up a cell and gene therapy approach to cure the Hemophilia A phenotype. We use endothelial cells from hepatic sinusoid or mesenchymal stem cells. Moreover we have started to reprogram cells from cutaneous bioposies from hemophilic patients, to correct them through gene therapy by means of Lentiviral vectors and to differentiate them towards endothelial cells to be transplanted in immunocompromised hemophilic mice to verify their engraftment, proliferation and correction of the hemorragic phenotype.
Biomaterials for osseointegration , bone and dental tissue engineering (inorganic/organic composites and materials functionalized with biologically active moieties -polypeptides, enzymes, growth factors and components of the extracellular matrix) are developed and characterized from various point of view (morphology, surface chemical-physical properties, mechanical performances, cells and tissue responses, antigens recognition according to the final clinical destination). Moreover, functionalized carriers for targeted delivery of anticancer drug, namely nanocomposites including inorganic materials (carbonate-calcium phosphate) and antibodies are being developed and characterized.


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Production of monoclonal antibodies
  • Lentiviral Vectors
  • Functionalization of nanoparticles
  • Isolation and characterization of adult stem cells from different tissues
  • Gene and Cell Therapy of Hemophilia A
  • Differentiation of adult stem cells
  • Riprogramming of adult somatic cells to iPS (induced pluripotent stem cells)

STRUMENTAZIONE

Denominazione

Struttura ove la strumentazione è allocata

Responsabile della strumentazione

ULTRACENTRIFUGE BECKMAN

Dipartimento Scienze Mediche

Dr. Enzo Perucco

REAL TIME PCR APPARATUS

Dipartimento Scienze Mediche

Dr. Enzo Perucco

COFOCAL MICROSCOPE APPARATUS

Dipartimento Scienze Mediche

Dr. Enzo Perucco

CITOFLUORIMETRO A FLUSSO

Dipartimento Scienze Mediche

Dr. Anna Rapa

IMAGE STATION 440 CF FOR DETECTION AND QUANTIFICATION OF AND

FLUORESCENCE

Dipartimento Scienze Mediche

Prof. Ciro Isidoro

DNA SEQUENCING APPARATUS

Dipartimento Scienze Mediche

Dr. Anna Rapa


PUBBLICAZIONI

Pietronave S, Forte G, Locarno D, Merlin S, Zamperone A, Nicotra G, Isidoro C, Di Nardo P, Prat M. Agonist monoclonal antibodies against the HGF-receptor protect cardiac muscle cells from apoptosis. Am J Physiol Heart Circ Physiol. 2010; 298(4):H1155-65

Pietronave S, Iafisco M, Locarno D, Rimondini L, Prat M. Functionalized nanomaterials for diagnosis and therapy of cancer. Journal of Applied Biomaterials & Biomechanics. 2009; 7:77-89.

Forte G, Minieri M, Cossa P, Antenucci D, Sala M, Gnocchi V, Fiaccavento R, Carotenuto F, De Vito P, Baldini PM, Prat M, Di Nardo P. Hepatocyte growth factor effects on mesenchymal stem cells: proliferation, migration, and differentiation. Stem Cells. 2006 Jan;24(1):23-33.

Zanetta M, Quirici N, Demarosi F, Tanzi MC, Rimondini L, Farè S. Ability of polyurethane foams to support cell proliferation and the differentiation of MSCs into osteoblasts. Acta Biomater. 2009 May;5(4):1126-36

Borsari V, Fini M, Giavaresi G, Tschon M, Chiesa R, Chiusoli L, Salito A, Rimondini L, Giardino R. Comparative in vivo evaluation of porous and dense duplex titanium and hydroxyapatite coating with high roughnesses in different implantation environments. J Biomed Mater Res A. 2009 May;89(2):550-60

Chiapasco M, Zaniboni M, Rimondini L. Dental implants placed in grafted maxillary sinuses: a retrospective analysis of clinical outcome according to the initial clinical situation and a proposal of defect classification. Clin Oral Implants Res. 2008 Apr;19(4):416-28.

Salvucci O, Maric D, Economopoulou M, Sakakibara S, Merlin S, Follenzi A, Tosato G. EphrinB reverse signaling contributes to endothelial and mural cell assembly into vascular structures. Blood. 2009 Aug 20;114(8):1707-16.


Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation. Inada M, Follenzi A, Cheng K, Surana M, Joseph B, Benten D, Bandi S, Qian H, Gupta S. J Cell Sci. 2008 Apr 1;121:1002-13

Follenzi A, Benten D, Novikoff P, Faulkner L, Raut S, Gupta S. Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice. J Clin Invest. 2008 Mar;118(3):935-45.

Follenzi A, Battaglia M, Lombardo A, Annoni A, Roncarolo MG, Naldini L.Targeting lentiviral vector expression to hepatocytes limits transgene-specific immune response and establishes long-term expression of human antihemophilic factor IX in mice. Blood. 2004 May 15;103(10):3700-9.


DOTTORATI DI RICERCA

Componente U.O.

Dottorato di Ricerca

Coordinatore

Sede

Maria Prat

Biotechnologies for human health

Prof. Claudio Santoro

Novara

Lia Rimondini

Biotechnologies for human health

Prof. Claudio Santoro

Novara

Antonia Follenzi

Biotechnologies for human health

Prof. Claudio Santoro

Novara


CONGRESSI C.I.B.

Congressi Partecipazione
CNB4
si
CNB5

CNB6
si
CNB7

CNB8
si
CNB9
CNB10