Santoro Claudio Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Santoro Claudio
Qualifica PO
Facoltà Scienze M.F.N.
Dipartimento
Scienze Mediche
Settore Scientifico Disciplinare BIO/13 Biologia Applicata
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PERSONALE STRUTTURATO

Cognome e Nome
Bozzo Chiarella
Qualifica RU
Dipartimento Medicina Clinica e Sperimentale
Ente di appartenenza UPO
Cognome e Nome Sblattero Daniele
Qualifica PA
Dipartimento Scienze Mediche
Ente di appartenenza UPO
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome Cotella Diego
Qualifica
Ass
Dipartimento
Scienze Mediche
Ente di appartenenza
UPO
Cognome e Nome
D’Angelo Sara
Qualifica
Dottoranda
Dipartimento
Scienze Mediche
Ente di appartenenza
UPO
Cognome e Nome Maione Federica
Qualifica Borsista
Dipartimento
Scienze Mediche
Ente di appartenenza
UPO
Cognome e Nome
Secco Paola
Qualifica
Ass
Dipartimento
Scienze Mediche
Ente di appartenenza
UPO
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

Antigen protein microarray
Despite intensive research, the etiology and the pathogenesis of the type 1 diabetes (T1DM) are still largely undetermined. Studies in animal models of spontaneous diabetes have shown that CD4+ and/or CD8+ cytotoxic T lymphocytes (CTL) directed against islet -cells become activated, expand clonally and entrain a cascade of processes (insulitis) culminating in -cell destruction. Moreover, a large fraction of type 1 diabetics have circulating auto-antibodies directed against islet specific antigens such as insulin (I), glutamic acid decarboxylase (GAD), and tyrosine phoshatase (IA-2). The search for the autoantigen(s) is crucially important both for understanding their role in regulation of the events occurring in pancreatic islets and for the elaboration of any antigen-specific based immunotherapy for T1DM. A possibility is given by the expression of cDNA fragments from target tissues or microorganisms. Although mapping sera with peptide phage libraries has had some success with small viral genomes such as hepatitis B, the selection of peptide repertoires appears not to be a suitable approach when profiling the complexity of the immune response in the context of the human genome. A more successful approach is to display cDNA expression libraries on filamentous phage. This his particularly true for autoimmune diseases, in which the source of cDNA may be the tissue recognized by the immune response. Recently we described an approach to filter out DNA encoding functional open reading frames from non-coding DNA. This approach is applicable to the efficient selection of random open reading frames representing the coding potential of whole organisms, and their subsequent downstream use in a number of different systems, in particular the production of protein arrays representative of the autoantigenic repertoire associated to T1DM.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Phage display
  • Expression of recombinant protein
  • Diabetes
  • Protein complementation assay
  • Protein array
  • Autoimmunity

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
SMART system
Dip. Sci. Mediche
Ezio Perucco
AKTA
Dip. Sci. Mediche
Ezio Perucco
Calligrapher BIO-Rad
Dip. Sci. Mediche
D’Angelo Sara
FACSalibur
Dip. Sci. Mediche
Bozzo Chiarella
Confocale Leica
Dip. Sci. Mediche
Bozzo Chiarella
2D system
Dip. Sci. Mediche
Chiocchetti Annalisa

PUBBLICAZIONI

Sblattero D, Ventura A, Tommasini A, Cattin L, Martelossi S, Florian F, Marzari R, Bradbury A, Not T. Cryptic gluten intolerance in type 1 diabetes: identifying suitable candidates for a gluten free diet. Gut. 2006 Jan;55(1):133-4. No abstract available.

Sblattero D, Maurano F, Mazzarella G, Rossi M, Auricchio S, Florian F, Ziberna F, Tommasini A, Not T, Ventura A, Bradbury A, Marzari R, Troncone R. Characterization of the anti-tissue transglutaminase antibody response in nonobese diabetic mice. J Immunol. 2005 May 1;174(9):5830-6.

Sblattero D, Florian F, Azzoni E, Ziberna F, Tommasini A, Not T, Ventura A, Bradbury A, Marzari R. One-step cloning of anti tissue transglutaminase scFv from subjects with celiac disease. J Autoimmun. 2004 Feb;22(1):65-72.

Zacchi P, Sblattero D, Florian F, Marzari R, Bradbury AR. Selecting open reading frames from DNA. Genome Res. 2003 May;13(5):980-90.

Chiocchetti A, Gibello L, Carando A, Aspesi A, Secco P, Garelli E, Loreni F, Angelini M, Biava A, Dahl N, Dianzani U, Ramenghi U, Santoro C, Dianzani I. Interactions between RPS19, mutated in Diamond-Blackfan anemia, and the PIM-1 oncoprotein. Haematologica. 2005 Nov;90(11):1453-62.

Collavin L, Gostissa M, Avolio F, Secco P, Ronchi A, Santoro C, Del Sal G. Modification of the erythroid transcription factor GATA-1 by SUMO-1. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8870-5. Epub 2004 Jun 1.

Bozzo C, Lombardi G, Santoro C, Canonico PL. Involvement of beta(1) integrin in betaAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci. 2004 Jan;25(1):1-8.

Secco P, Ferretti M, Gioia D, Cesaro P, Bozzo C, Marks JD, Santoro C. Characterization of a single-chain intrabody directed against the human receptor tyrosine kinase Ron. J Immunol Methods. 2004 Feb 1;285(1):99-109.

Secco P, Cotella D, Santoro C. Selection of peptides with affinity for the N-terminal domain of GATA-1: Identification of a potential interacting protein. Biochem Biophys Res Commun. 2003 Jun 13;305(4):1061-6.

Cotella D, Jost N, Darna M, Radicke S, Ravens U, Wettwer E. Silencing the cardiac potassium channel Kv4.3 by RNA interference in a CHO expression system. Biochem Biophys Res Commun. 2005 May 6;330(2):555-60.

 


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Santoro Claudio
Biotecnologie per l’uomo
Santoro Claudio
UPO
Bozzo Chiarella
Medicina Clinica e sperimentale
Gariglio Marisa
UPO

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10