Roberti Marina Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome ROBERTI Marina
Qualifica ASSOCIATE PROFESSOR
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Settore Scientifico Disciplinare BIO/10
E-mail Questo indirizzo e-mail è protetto dallo spam bot. Abilita Javascript per vederlo.

PERSONALE STRUTTURATO

Cognome e Nome Attimonelli Marcella
Qualifica ASSOCIATE PROFESSOR
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"
Cognome e Nome Lezza M.S. Angela
Qualifica ASSISTANT PROFESSOR (RICERCATORE UNIVERSITA’ RTI)
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"
Cognome e Nome Pesce Vito
Qualifica ASSISTANT PROFESSOR (RICERCATORE UNIVERSITA’ RTI)
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"
Cognome e Nome Cormio Antonella
Qualifica ASSISTANT PROFESSOR (RICERCATORE UNIVERSITA’ RTI)
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"
Cognome e Nome Loguercio Polosa Paola
Qualifica ASSISTANT PROFESSOR (RICERCATORE UNIVERSITA’ RTI)
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"
Cognome e Nome BRUNI FRANCESCO
Qualifica ASSISTANT PROFESSOR (RICERCATORE UNIVERSITA’ RTD B)
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"

PERSONALE NON STRUTTURATO

Cognome e Nome Capriglia Francesco
Qualifica PHD STUDENT
Dipartimento Biosciences, Biotechnologies and Biopharmaceutics
Ente di appartenenza University of Bari "Aldo Moro"

LINEE DI RICERCA

Design and implementation of databases and tools for the study of the human mitochondrial variability in health and disease. At this aim the databases HmtDB (https://www.hmtdb.uniba.it/hmdb/), HmtVar (https://www.hmtvar.uniba.it) and HmtPhenome (this last will be soon implemented), as well as MToolBox (https://github.com/mitoNGS/MToolBox) and MewAS packages (this last in progress) have been developed, kept updated and used to assess pathogenicity of human mtDNA variants as well as to recognize their interactions with nuclear genes directed to mitochondrion.

Study of the role of mitochondria and the mitochondrial genetic system in the aging process and in age-related pathological conditions (sarcopenia, diabetes, neurodegenerative diseases, cancer etc) as well as of the nutritional strategies or drugs that may prevent the degenerative processes, eventually promoting mitochondrial biogenesis.

 

Study of nuclear factors involved in the signaling pathways of mitochondrial biogenesis (PGC1s, Akt, SIRTs). Determination and quantification of mtDNA damages, as well as analysis of mtDNA stability and TFAM-mtDNA interactions. Experimental models are: i) tissue samples from rats and humans of different ages (collaboration with Prof. R. Bernabei, Univ. Cattolica - Sacro Cuore, Roma), ii) rats treated with caloric restriction diet (collaboration with Prof. C. Leeuwenburgh, Univ. of Florida, Gainesville, FL, USA) or compounds qualified as nutraceuticals, iii) cancer tissues samples.

 

Studies on pathogenetic mechanisms responsible for the Leber Hereditary Optic Neuropathy. Role of mitochondrial biogenesis in modulating the disease penetrance and as predictive tool for the clinical manifestation of the disease.

Studies on mitochondrial tRNA related diseases (MELAS and MERRF): evaluation of tissue-specific effects and rescuing mechanisms of therapeutic molecules derived from mitochondrial leucyl tRNA synthetase (collaboration with prof. G. d’Amati, Università La Sapienza, Roma).

Study of mitochondrial DNA replication and transcription machinery in animal systems, aimed at the identification and characterization of new regulatory factors/enzymes (collaboration with prof. N.-G. Larsson, Dept of Med. Biochem. and Biophys., Karolinska Institutet, Stockholm, Sweden). A recent publication identifies Mgme1 exonuclease as a crucial regulator of mitochondrial DNA replication, as shown by mitochondrial pathologies caused by mutations in this gene.




TECNOLOGIE IN POSSESSO DELL'U. O.

  • Cloning expression and purification of proteins in different eukaryotic and prokaryotic systems
  • Analysis of protein-protein and protein-DNA interactions: Chromatin Immunoprecipitation (nuclear and mitochondrial), in vivo proximity labelling
  • Spectrophotometric analysis of enzymatic activities
  • Usage of mitochondrial data Banks
  • Sequence analysis. DNA mutations screening
  • Isolation characterization and quantification of DNA and RNA (PCR, RT-PCR, real-time PCR
  • Cell culture (mammalian cells and insect cells); creation and/or maintenance of stably and transiently transfected cell lines
  • Histochemical analysis of tissues micro-cryosections and Laser capture of single cells followed by Real Time  PCR
  • Compilation of the NumtS sequences in Human and other mammals in the genome browser UCSC
  • Two-dimensional gel electrophoresis. Blue Native gel electrophoresis Western blotting and immunodetection.

STRUMENTAZIONE

Denominazione

Struttura ove la strumentazione è allocata

Responsabile della strumentazione

ChemidocTM MP Imaging System (BioRad)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

A. Cormio

Imaging System Typhoon FLA 9500 (GE Healthcare)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

F. Bruni

Server MacPRO

Dept of Biosciences, Biotechnologies and Biopharmaceutics

M. Attimonelli

Cryostat microtome (Microm)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

V. Pesce

Bidimensional electrophoresis apparatus (Amersham)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

A. Cormio

Real Time PCR apparatus QuantStudio7Flex (Life Technologies)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

V. Pesce

Cell Cultures Facility

Dept of Biosciences, Biotechnologies and Biopharmaceutics

P. Loguercio Polosa

FPLC System for protein chromatography (BioRad)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

P. Loguercio Polosa

Calorimeter

MicroCal™ iTC200 System (GE Healthcare)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

M. Roberti

Fluorescence Microscopes System (Zeiss)

Dept of Biosciences, Biotechnologies and Biopharmaceutics

V. Pesce


PUBBLICAZIONI

Increased TFAM binding to mtDNA damage hot spots is associated with mtDNA loss in aged rat heart.

Chimienti G, Picca A, Sirago G, Fracasso F, Calvani R, Bernabei R, Russo F, Carter CS, Leeuwenburgh C, Pesce V, Marzetti E, Lezza AMS

Free Radic Biol Med. 2018 Aug 20; 124:447-453. doi:10.1016/j.freeradbiomed.2018.06.041.

 

Aging and calorie restriction oppositely affect mitochondrial biogenesis through TFAM binding at both origins of mitochondrial DNA replication in rat liver.

Picca A, Pesce V, Fracasso F, Joseph AM, Leeuwenburgh C, Lezza AMS

PLoS ONE, 2013, 8(9): e74644; doi:10.1371/journal.pone.0074644

 

Clinical, biochemical, and genetic features associated with VARS2-related mitochondrial disease.

Bruni F, Di Meo I, Bellacchio E, Webb BD, McFarland R, Chrzanowska-Lightowlers ZMA, He L, Skorupa E, Moroni I, Ardissone A, Walczak A, Tyynismaa H, Isohanni P, Mandel H, Prokisch H, Haack T, Bonnen PE, Enrico B, Pronicka E, Ghezzi D, Taylor RW, Diodato D.

Hum Mutat. 2018 Apr;39(4):563-578. doi: 10.1002/humu.23398.

 

Toward the Standardization of Mitochondrial Proteomics: The Italian Mitochondrial Human Proteome Project Initiative

Alberio T, Pieroni L, Ronci M, Banfi C, Bongarzone I, Bottoni P, Brioschi M, Caterino M, Chinello C, Cormio A, Cozzolino F, Cunsolo V, Fontana S, Garavaglia B, Giusti L, Greco V, Lucacchini A, Maffioli E, Magni F, Monteleone F, Monti M, Monti V, Musicco C, Petrosillo G, Porcelli V, Saletti R, Scatena R, Soggiu A, Tedeschi G, Zilocchi M, Roncada P, Urbani A, Fasano M

J Proteome Res. 2017 16: 4319-4329.28. doi: 10.1021/acs.jproteome.7b00350

 

Inhibition of Lon protease by triterpenoids alters mitochondria and is associated to cell death in human cancer cells.

Gibellini L, Pinti M, Bartolomeo R, De Biasi S, Cormio A, Musicco C, Carnevale G, Pecorini S, Nasi M, De Pol A, Cossarizza A.

Oncotarget 2015 6: 25466-25483  doi: 10.18632/oncotarget.4510

 

HmtVar: a new resource for human mitochondrial variations and pathogenicity data.

Preste R, Vitale O, Clima R, Gasparre G, Attimonelli M.

Nucleic Acids Res.2019 Jan 8;47(D1):D1202-D1210. doi: 10.1093/nar/gky1024. PubMed PMID: 30371888;PubMed Central PMCID: PMC6323908.

 

MSeqDR mvTool: A mitochondrial DNA Web and API resource for comprehensive variant annotation, universal nomenclature collation, and reference genome conversion.

Shen L, Attimonelli M, Bai R, Lott MT, Wallace DC, Falk MJ, Gai X.

Hum Mutat. 2018 Jun;39(6):806-810. doi: 10.1002/humu.23422. Epub 2018 Apr 6. PubMed PMID: 29539190; PubMed Central PMCID: PMC5992054.

 

Cigarette toxicity triggers Leber’s hereditary optic neuropathy by affecting mtDNA copy number, oxidative phosphorylation and ROS detoxification pathways.

Giordano L., Deceglie S., d’Adamo P., Valentino M.L., La Morgia C., Fracasso F., Roberti M., Cappellari M., Petrosillo G., Ciaravolo S., Parente D., Giordano C., Maresca A., Iommarini L., Del Dotto V., Ghelli A.M., Salomao S.R., Berezovsky A., Belfort Jr R., Sadun A.A., Carelli V., Loguercio Polosa P., Cantatore P

Cell Death and Disease (2015) 6, e2021  doi: 10.1038/cddis.2015.364.

 

The sea urchin mitochondrial transcription factor a binds and bends DNA efficiently despite its unusually short c-terminal tail.

Malarkey C.S., Lionetti C., Deceglie S., Roberti M., Churchill M.E.A., Cantatore P., Loguercio Polosa P.

Mitochondrion (2016) 29, 1-6. doi: 10.1016/j.mito.2016.04.004.

 

Mice lacking the mitochondrial exonuclease MGME1 accumulate mtDNA deletions without developing progeria

Matic S, Jiang M,. Nicholls TJ,. Uhler JP, Dirksen-Schwanenland C, Loguercio Polosa P, Simard M-L, Li X, Atanassov I, Rackham O, Filipovska A, Stewart JB, Falkenberg M, Larsson N-G, Milenkovic D

Nature Communications (2018) 9(1):1202. doi: 10.1038/s41467-018-03552-x.

 


DOTTORATI DI RICERCA

Componente U.O.

Dottorato di Ricerca

Coordinatore

Sede

Attimonelli M.

genomica e proteomica funzionale e applicata

Prof. M. Svelto

Dept of Biosciences, Biotechnologies and Pharmacological Sciences

University of Bari

Loguercio Polosa P. genomica e proteomica funzionale e applicata

Prof. M. Svelto

Dept of Biosciences, Biotechnologies and Pharmacological Sciences

University of Bari

 

Pesce V.

genomica e proteomica funzionale e applicata

Prof. M. Svelto

Dept of Biosciences, Biotechnologies and Pharmacological Sciences
University of Bari
Cormio A. genomica e proteomica funzionale e applicata

Prof. M. Svelto

Dept of Biosciences, Biotechnologies and Pharmacological Sciences
University of Bari

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4
M. Volpicella, LR Ceci, R. Gallerani
CNB5
F. De Leo, R. Gallerani, M. Volpicella, LR Ceci
CNB6
M. Volpicella, F. De Leo, R. Gallerani, LR Ceci
CNB7
M. Volpicella, LR Ceci, F. De Leo, M. Sciancalepore, M. Losacco, R. Gallerani
CNB8
M. Losacco, F. Pisani, R. Gallerani, F. De Leo
CNB9 R. Gallerani,  F. De Leo, LR. Ceci
CNB10 F. De Leo, LR, Ceci, R. Gallerani, C. Leoni.