-Peptide nucleic acids as tool for the development of innovative protocols in the diagnosis of genetic diseases using SPR technologies: β-thalassemia, cystic fibrosis, sickle-cell disease.

-Non-invasive prenatal diagnosis of single point mutations causing inherited genetic disease using advanced technologies such as droplet digital PCR and SPR-based biosensors.

-Biological and diagnostic applications of dielectrophoresis (DEP) based Lab-on-a-chip devices to diagnosis and drug research and development.

-Identification of novel compounds as inducers of erythroid differentiation for β-thalassemia.

-Analysis of anti-inflammatory molecules and inflammatory pathways for cystic fibrosis, COPD disease and Shwachman-Diamond syndrome.

-Read-through strategy for the treatments of genetic rare diseases caused by nonsense mutations such as cystic fibrosis and Shwachman-Diamond syndrome.

-Analysis of the effects in vivo of molecules of interest in the therapy of β-thalassemia: the zebrafish model.

-Altered erythroid-related miRNA levels as a possible novel marker for detection of autologous blood transfusion misuse in sport.

-Liquid biopsy for the identification of miRNA pattern in colon cancer patients: miR141, miR-221 and miR-222 as target sequences.

-Next generation sequencing of miRNA plasma levels in colon cancer patients.

-Identification and characterization of antibacterial low molecular weight molecules inhibiting Pseudomonas aeruginosa in cystic fibrosis.

-Development of transgenic mice carrying β-thalassemia genetic mutations: β0-39, β-IVSI-6 and β-IVSI-110.

Procaryotic and eukaryotic cell cultures

Primary mammalian cell cultures

Single Cell Cloning

Reporter gene assays

Secretomic Analysis

Nucleic acid quantification: qRT-PCR, ddPCR

Flow cytometry

Microscopy and Live Cell Screening Systems

SPR technology

HPLC

1: Breveglieri G, D'Aversa E, Gallo TE, Pellegatti P, Guerra G, Cosenza LC, Finotti A, Gambari R, Borgatti M. A novel and efficient protocol for Surface Plasmon Resonance based detection of four Β-thalassemia point mutations in blood samples and salivary swabs. Sensors and Actuators, B: Chemical. 2018; 260:710-718. doi: 10.1016/j.snb.2017.12.209.

2: Fabbri E, Tamanini A, Jakova T, Gasparello J, Manicardi A, Corradini R, Sabbioni G, Finotti A, Borgatti M, Lampronti I, Munari S, Dechecchi MC, Cabrini G, Gambari R. A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells. Molecules. 2017 Dec 29;23(1). pii: E71. doi:

10.3390/molecules23010071.

3: Gasparello J, Fabbri E, Bianchi N, Breveglieri G, Zuccato C, Borgatti M, Gambari R, Finotti A. BCL11A mRNA Targeting by miR-210: A Possible Network Regulating γ-Globin Gene Expression. Int J Mol Sci. 2017 Nov 26;18(12). pii: E2530. doi: 10.3390/ijms18122530.

4: Breveglieri G, Travan A, D'Aversa E, Cosenza LC, Pellegatti P, Guerra G, Gambari R, Borgatti M. Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays. PLoS One. 2017 Feb 24;12(2):e0172756. doi: 10.1371/journal.pone.0172756. eCollection 2017.

5: Montagner G, Bezzerri V, Cabrini G, Fabbri E, Borgatti M, Lampronti I, Finotti A, Nielsen PE, Gambari R. An antisense peptide nucleic acid against Pseudomonas aeruginosa inhibiting bacterial-induced inflammatory responses in the cystic fibrosis IB3-1 cellular model system. Int J Biol Macromol. 2017 Jun;99:492-498. doi: 10.1016/j.ijbiomac.2017.02.011.

6: Breveglieri G, Gallo TE, Travan A, Pellegatti P, Guerra G, Gambari R, Borgatti M. Surface plasmon resonance analysis to detect the β(+)IVSI-110 thalassemia mutation in circulating cell-free fetal DNA. Clin Chim Acta. 2016 Nov 1;462:133-134. doi: 10.1016/j.cca.2016.09.008.

7: Cosenza LC, Breda L, Breveglieri G, Zuccato C, Finotti A, Lampronti I, Borgatti M, Chiavilli F, Gamberini MR, Satta S, Manunza L, De Martis FR, Moi P, Rivella S, Gambari R, Bianchi N. A validated cellular biobank for β-thalassemia.  J Transl Med. 2016 Sep 2;14:255. doi: 10.1186/s12967-016-1016-4.

8: Altamura E, Borgatti M, Finotti A, Gasparello J, Gambari R, Spinelli M, Castaldo R, Altamura N. Chemical-Induced Read-Through at Premature Termination Codons Determined by a Rapid Dual-Fluorescence System Based on S. cerevisiae. PLoS One. 2016 Apr 27;11(4):e0154260. doi: 10.1371/journal.pone.0154260.

9: Breveglieri G, Bassi E, Carlassara S, Cosenza LC, Pellegatti P, Guerra G, Finotti A, Gambari R, Borgatti M. Y-chromosome identification in circulating cell-free fetal DNA using surface plasmon resonance. Prenat Diagn. 2016 Apr;36(4):353-61. doi: 10.1002/pd.4788.

10: Brognara E, Fabbri E, Montagner G, Gasparello J, Manicardi A, Corradini R, Bianchi N, Finotti A, Breveglieri G, Borgatti M, Lampronti I, Milani R, Dechecchi MC, Cabrini G, Gambari R. High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222. Int J Oncol. 2016 Mar;48(3):1029-38. doi: 10.3892/ijo.2015.3308.