-Non-invasive prenatal diagnosis of single point mutations causing inherited genetic disease using advanced technologies such as droplet digital PCR and SPR-based biosensors.

-Investigation of signaling pathway and secretomic profile in Shwachman-Diamond Syndrome.

-Non coding RNA modulation as innovative therapeutic strategy in thalassemia and cystic fibrosis.

-Identification of novel compounds as inducers of erythroid differentiation for β-thalassemia and sickle cell disease.

-Analysis of the effects in vivo of molecules of interest in the therapy of β-thalassemia: the zebrafish model.

-Analysis of anti-inflammatory molecules and inflammatory pathways for cystic fibrosis, COPD disease and Shwachman-Diamond syndrome.

- Identification of novel anti-inflammatory agents and CFTR-modulators for cystic fibrosis.

-Read-through strategy for the treatments of genetic rare diseases caused by nonsense mutations such as cystic fibrosis and Shwachman-Diamond syndrome.

-Peptide nucleic acids as tool for the development of innovative protocols in the diagnosis of genetic diseases using SPR technologies: β-thalassemia, cystic fibrosis, sickle-cell disease.

-Biological and diagnostic applications of dielectrophoresis (DEP) based Lab-on-a-chip devices to diagnosis and drug research and development.

-Studies on the anti-proliferative and pro-apoptotic activities of natural or synthetic derivatives.

Procaryotic and eukaryotic cell cultures

Primary mammalian cell cultures

Single Cell Cloning

Reporter gene assays

Secretomic Analysis

Nucleic acid quantification: qRT-PCR, ddPCR

Flow cytometry

Microscopy and Live Cell Screening Systems

SPR technology

HPLC

Breveglieri G, Pacifico S, Zuccato C, Cosenza LC, Sultan S, D'Aversa E, Gambari R, Preti D, Trapella C, Guerrini R, Borgatti M. Discovery of Novel Fetal Hemoglobin Inducers through Small Chemical Library Screening. Int J Mol Sci. 2020 Oct 8;21(19):7426. doi: 10.3390/ijms21197426. PMID: 33050052; PMCID: PMC7582302.

Breveglieri G, Travan A, D'Aversa E, Cosenza LC, Pellegatti P, Guerra G, Gambari R, Borgatti M. Postnatal and non-invasive prenatal detection of β-thalassemia mutations based on Taqman genotyping assays. PLoS One. 2017 Feb 24;12(2):e0172756. doi: 10.1371/journal.pone.0172756. PMID: 28235086; PMCID: PMC5325530.

Vella A, D'Aversa E, Api M, Breveglieri G, Allegri M, Giacomazzi A, Marinelli Busilacchi E, Fabrizzi B, Cestari T, Sorio C, Bedini G, D'Amico G, Bronte V, Poloni A, Benedetti A, Bovo C, Corey SJ, Borgatti M, Cipolli M, Bezzerri V. mTOR and STAT3 Pathway Hyper-Activation is Associated with Elevated Interleukin-6 Levels in Patients with Shwachman-Diamond Syndrome: Further Evidence of Lymphoid Lineage Impairment. Cancers (Basel). 2020 Mar 5;12(3):597. doi: 10.3390/cancers12030597. PMID: 32150944; PMCID: PMC7139896.

Sultan S, Rozzi A, Gasparello J, Manicardi A, Corradini R, Papi C, Finotti A, Lampronti I, Reali E, Cabrini G, Gambari R, Borgatti M. A Peptide Nucleic Acid

(PNA) Masking the miR-145-5p Binding Site of the 3'UTR of the Cystic Fibrosis

Transmembrane Conductance Regulator (CFTR) mRNA Enhances CFTR Expression in Calu-3 Cells. Molecules. 2020 Apr 5;25(7):1677. doi:

10.3390/molecules25071677. PMID: 32260566; PMCID: PMC7181265.

Borgatti M, Altamura E, Salvatori F, D'Aversa E, Altamura N. Screening Readthrough Compounds to Suppress Nonsense Mutations: Possible Application to β-Thalassemia. J Clin Med. 2020 Jan 21;9(2):289. doi: 10.3390/jcm9020289. PMID:

31972957; PMCID: PMC7073686.

Breveglieri G, D'Aversa E, Cosenza LC, Boutou E, Balassopoulou A, Voskaridou

E, Gambari R, Borgatti M. Detection of the sickle hemoglobin allele using a surface plasmon resonance based biosensor. Sens Actuators B Chem. 2019 Oct 1;296:126604. doi: 10.1016/j.snb.2019.05.081. PMID: 31853166; PMCID: PMC6894342.

Breveglieri G, D'Aversa E, Finotti A, Borgatti M. Non-invasive Prenatal Testing Using Fetal DNA. Mol Diagn Ther. 2019 Apr;23(2):291-299. doi: 10.1007/s40291-019-00385-2. PMID: 30712216.

D'Aversa E, Breveglieri G, Pellegatti P, Guerra G, Gambari R, Borgatti M. Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR. Mol Med. 2018 Apr 5;24(1):14. doi: 10.1186/s10020-018-0016-7. PMID: 30134789; PMCID: PMC6016869.

Lampronti I, Dechecchi MC, Rimessi A, Bezzerri V, Nicolis E, Guerrini A, Tacchini M, Tamanini A, Munari S, D'Aversa E, Santangelo A, Lippi G, Sacchetti G, Pinton P, Gambari R, Agostini M, Cabrini G. β-Sitosterol Reduces the Expression of Chemotactic Cytokine Genes in Cystic Fibrosis Bronchial Epithelial Cells. Front Pharmacol. 2017 May 12;8:236. doi: 10.3389/fphar.2017.00236. eCollection 2017.PMID: 28553226

Lampronti I, Manzione MG, Sacchetti G, Ferrari D, Spisani S, Bezzerri V, Finotti A, Borgatti M, Dechecchi MC, Miolo G, Marzaro G, Cabrini G, Gambari R, Chilin A. Differential Effects of Angelicin Analogues on NF- κ B Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells. Mediators Inflamm. 2017;2017:2389487. doi: 10.1155/2017/2389487. Epub 2017 Sep 27.PMID: 29089668