Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Parolini Silvia Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Parolini Silvia
Qualifica Professore Associato
Facoltà Università di Medicina e Chirurgia
Dipartimento Scienze Biomediche e Biotecnologie
Settore Scientifico Disciplinare BIO/17
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PERSONALE STRUTTURATO

Cognome e Nome Tabellini Giovanna
Qualifica Ricercatore
Dipartimento Scienze Biomediche e Biotecnologie
Ente di appartenenza Università degli studi di Brescia
Cognome e Nome Coltrini Daniela
Qualifica Ricercatore
Dipartimento Scienze Biomediche e Biotecnologie
Ente di appartenenza Università degli studi di Brescia
Cognome e Nome Benassi Marzia
Qualifica Tecnico laureato
Dipartimento Scienze Biomediche e Biotecnologie
Ente di appartenenza Università degli studi di Brescia
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Dipartimento
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PERSONALE NON STRUTTURATO

Cognome e Nome Patrizi Ornella
Qualifica Assegnista
Dipartimento Scienze Biomediche e Biotecnologie
Ente di appartenenza Università degli studi di Brescia
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Dipartimento
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LINEE DI RICERCA

Human Natural Killer cells (NK) were originally identified on the capability of killing certain tumor cell lines in the absence of deliberate previous stimulation. Recentlys a novel concept has emerged indicating that the actual role of NK cells is not only confined to the destruction of virus-infected cells or tumors.

This is particularly relevant during the early phases of acute inflammation, secondary to infection. Various studies were focused on the crosstalk between NK cells and monocyte-derived dendritic cells and more recently on the involvement of plasmocytoid dendritic cells, mast cells, basophils, eosinophils, and neutrophils. This is in accord with the fact that NK cells from blood can be attracted to peripheral tissues by several chemokines released during inflammatory responses. In contrast, a minority of NK cells from blood expresses receptors for homing to lymphoid tissues.

In particular the function of NK cells is regulated by a balance between opposite signals delivered by a set of HLA class I specific inhibitory receptors and by a number of activating receptors and co-receptors responsible for NK cell triggering. By the combined use of these receptors, NK cells can discriminate between normal HLA class I+ cells and cells that have lost the expression of HLA class I molecules as a consequence of tumor transformation or viral infection.

For over a decade, our research group has been studying NK cells analysis identifying novel functional molecules expressed on different subsets of NK cells and investigating NK cells derived from patients affected by primary immune deficiencies.

Recently, in our laboratory, we also analyze the expression pattern and the function of inhibitory and activating NK receptors in NK cells different subsets derived from patients with epithelial ovarian carcinoma subjected to primary surgery. A special focus is made on the interaction between inhibitory/activating NK receptors and HLA class I molecules/tumoural ligands to understand better NK-mediated lysis mechanisms that may reflect a differential involvement of these receptors in controlling the process of tumor growth.

These immunological analyses are possible thanks to the isolation in our laboratory of monoclonal antibodies that can be used in this particular type of experiments in which most commercially available reagents can not be used.

At last, our research group have identified novel NK cell receptors and markers allowing a further and better characterization of different human NK cells subsets, by the generation and isolation of monoclonal antibodies in our laboratory.


TECNOLOGIE IN POSSESSO DELL'U. O.

  • NK cells culture technique and NK cell cloning
  • Primary tumor cell lines cultures
  • Immunocyto- and histochemistry
  • Generation and isolation of hybridomas producing monoclonal antibodies
  • Cytotoxic functional assay
  • Cytotoxicity and cytokine production tests
  • Immunofluorescence analysis by flow cytometry
  • Confocal microscopy
  • Basic molecular biology
  • Real-Time PCR

STRUMENTAZIONE

Denominazione

Struttura ove la strumentazione è allocata

Responsabile della strumentazione

Gamma-Counter

Dipartimento di Scienze Biomediche e Biotecnologie

Tabellini G.

FacsCanto BD

Dipartimento di Scienze Biomediche e Biotecnologie

Parolini S.

ZEISS Confocal Microscope

Dipartimento di Scienze Biomediche e Biotecnologie

Bresciani R

Bio-Rad I Cycler Real Time PCR

Dipartimento di Scienze Biomediche e Biotecnologie

Coltrini D.

 

 

 

 

 

 


PUBBLICAZIONI

Exome sequencing reveals a pallidin mutation in a Hermansky-Pudlak-like primary immunodeficiency syndrome.

Badolato R, Prandini A, Caracciolo S, Colombo F, Tabellini G, Giacomelli M, Cantarini ME, Pession A, Bell CJ, Dinwiddie DL, Miller NA, Hateley SL, Saunders CJ, Zhang L, Schroth GP, Plebani A, Parolini S, Kingsmore SF.

Blood. 2012 Mar 29;119(13):3185-7.

A novel primary human immunodeficiency due to deficiency in the WASP-interacting protein WIP.

Lanzi G, Moratto D, Vairo D, Masneri S, Delmonte O, Paganini T, Parolini S, Tabellini G, Mazza C, Savoldi G, Montin D, Martino S, Tovo P, Pessach IM, Massaad MJ, Ramesh N, Porta F, Plebani A, Notarangelo LD, Geha RS, Giliani S.

J Exp Med. 2012 Jan 16;209(1):29-34. Epub 2012 Jan 9.

NK cells and their receptors during viral infections.

Marcenaro E, Carlomagno S, Pesce S, Della Chiesa M, Parolini S, Moretta A, Sivori S.

Immunotherapy. 2011 Sep;3(9):1075-86. Review.

Severe impairment of IFN-γ and IFN-α responses in cells of a patient with a novel STAT1 splicing mutation.

Vairo D, Tassone L, Tabellini G, Tamassia N, Gasperini S, Bazzoni F, Plebani A, Porta F, Notarangelo LD, Parolini S, Giliani S, Badolato R.

Blood. 2011 Aug 18;118(7):1806-17. Epub 2011 Jul 19.

GPR56 as a novel marker identifying the CD56dull CD16+ NK cell subset both in blood stream and in inflamed peripheral tissues.

Della Chiesa M, Falco M, Parolini S, Bellora F, Petretto A, Romeo E, Balsamo M, Gambarotti M, Scordamaglia F, Tabellini G, Facchetti F, Vermi W, Bottino C, Moretta A, Vitale M.

Int Immunol. 2010 Feb;22(2):91-100. Epub 2009 Dec 14.

NK cells at the interface between innate and adaptive immunity.

Moretta A, Marcenaro E, Parolini S, Ferlazzo G, Moretta L.

Cell Death Differ. 2008 Feb;15(2):226-33. Epub 2007 Jun 1. Review.

Impact of VEGF-dependent tumour micro-environment on EDB fibronectin expression by subcutaneous human tumour xenografts in nude mice.

Coltrini D, Ronca R, Belleri M, Zardi L, Indraccolo S, Scarlato V, Giavazzi R, Presta M.

J Pathol. 2009 Dec;219(4):455-62.

Functional characterization of natural killer cells in type I leukocyte adhesion deficiency.

Castriconi R, Dondero A, Cantoni C, Della Chiesa M, Prato C, Nanni M, Fiorini M, Notarangelo L, Parolini S, Moretta L, Notarangelo L, Moretta A, Bottino C.

Blood. 2007 Jun 1;109(11):4873-81. Epub 2007 Feb 1.

The role of chemerin in the colocalization of NK and dendritic cell subsets into inflamed tissues.

Parolini S, Santoro A, Marcenaro E, Luini W, Massardi L, Facchetti F, Communi D, Parmentier M, Majorana A, Sironi M, Tabellini G, Moretta A, Sozzani S.

Blood. 2007 May 1;109(9):3625-32. Epub 2007 Jan 3.

Innate immunity defects in Hermansky-Pudlak type 2 syndrome.

Fontana S, Parolini S, Vermi W, Booth S, Gallo F, Donini M, Benassi M, Gentili F, Ferrari D, Notarangelo LD, Cavadini P, Marcenaro E, Dusi S, Cassatella M, Facchetti F, Griffiths GM, Moretta A, Notarangelo LD, Badolato R.

Blood. 2006 Jun 15;107(12):4857-64. Epub 2006 Feb 28.


DOTTORATI DI RICERCA

Componente U.O.

Dottorato di Ricerca

Coordinatore

Sede

Parolini Silvia

Genetica Molecolare applicata alle Scienze Mediche

De Petro G.

Brescia

 

 

 

 

 

 

 

 


CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10