Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Cantoni Orazio Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Cantoni Orazio
Qualifica Full Professor
Facoltà Faculty of Pharmacy
Dipartimento Institute of Pharmacology and Pharmacognosy
Settore Scientifico Disciplinare BIO/14
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PERSONALE STRUTTURATO

Cognome e Nome Balduini Walter
Qualifica PA
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome Guidarelli Andrea
Qualifica
RU
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome
Palomba Letizia
Qualifica
RU
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome Carloni Elisa
Qualifica
Ph.D. Student
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome
Carloni Silvia
Qualifica
Ph.D. Student
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome Cerioni Liana
Qualifica Post-Doctoral fellow
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome Tommasini Ilaria
Qualifica Post-Doctoral fellow (Assegnista)
Dipartimento
Institute of Pharmacology and Pharmacognosy
Ente di appartenenza University of Urbino
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

We are studying the mechanism(s) whereby cells belonging to the monocyte/macrophage lineage cope with peroxynitrite. We know that non-toxic concentrations of peroxynitrite nevertheless commit these cells to a rapid mitochondrial permeability transition-dependent death, that is however prevented by a signalling pathway leading to changes in the sub-cellular distributions of Bad. In this pathway, the sequential involvement of cytosolic phospholipase A2, 5-lipoxygenase and PKC alpha was demonstrated. We are currently investigating whether other pathways converge in the regulation of Bad localisation and whether the same survival mechanisms regulate viability of M1 macrophages (i.e. activated with bacterial products or Th1 cytokines) or M2 macrophages (i.e. activated by Th2 cytokines or immunosuppressors). The long-term target of our studies is the identification of mechanisms critically involved in the regulation of survival in polarised macrophages. These findings might then provide the ground for designing therapies for the deletion of specific macrophage-subsets (e.g. M1 macrophages in inflamed tissues).

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Western blotting
  • Immunocytochemistry (Cells and tissues)
  • DNA fragmentation analysis by conventional gel and programmable, autonomously controlled electrode electrophoresis
  • HPLC assays (e.g. ATP, NADPH, etc).
  • Transfection with oligonucleotides
  • Cytotoxicity assays
  • Techniques for the measurement of DNA damage (e.g. comet and alkaline Halo assays)
  • Assays using a microplate reader (e.g. for the determination of GSH, MTT reduction, etc.)
  • Assays using confocal or fluorescence microscopy (e.g. for the detection of NO, superoxide, hydrogen peroxide, etc.)
  • Spectrofotometric assays (e.g. for the determination of the activities ofcatalase, glutathione peroxidase, superoxide dismutase, etc. )

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Reversed-phase HPLC with UV detector (Beckman syst
Institute of Pharmacology and Pharmacognosy
Prof. Orazio Cantoni
Confocal microscope (Bio Rad DVC 250)
Institute of Pharmacology and Pharmacognosy
Prof. Orazio Cantoni
Fluorescence microscope (Olympus BX51)
Institute of Pharmacology and Pharmacognosy
Prof. Orazio Cantoni
Computer controlled microplate reader for absorban
Institute of Pharmacology and Pharmacognosy
Prof. Orazio Cantoni

PUBBLICAZIONI

1. Balduini W, De Angelis V, Mazzoni E, Cimino M. Stroke. 2001 Sep;32(9):2185-91. 2. Tommasini I, Sestili P, Cantoni O. Mol Pharmacol. 2002 Apr;61(4):870-8. 3. Tommasini I, Sestili P, Guidarelli A, Cantoni O. Cell Death Differ. 2002 Dec;9(12):1368-76. 4. Palomba L, Bianchi M, Persichini T, Magnani M, Colasanti M, Cantoni O. Free Radic Biol Med. 2004 Feb 1;36(3):319-29 5. Carloni S, Mazzoni E, Balduini W. J Neurochem. 2004 Apr;89(1):197-203. 6. Palomba L, Persichini T, Mazzone V, Colasanti M, Cantoni O. J Biol Chem. 2004 Jul 16;279(29):29895-901. Epub 2004 May 17. 7. Nisoli E, Falcone S, Tonello C, Cozzi V, Palomba L, Fiorani M, Pisconti A, Brunelli S, Cardile A, Francolini M, Cantoni O, Carruba MO, Moncada S, Clementi E. Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16507-12. 8. Tommasini I, Sestili P, Guidarelli A, Cantoni O. Cell Death Differ. 2004 Sep;11(9):974-84. 9. Nisoli E, Tonello C, Cardile A, Cozzi V, Bracale R, Tedesco L, Falcone S, Valerio A, Cantoni O, Clementi E, Moncada S, Carruba MO. Science. 2005 Oct 14;310(5746):314-7. 10. Guidarelli A, Cerioni L, Tommasini I, Fiorani M, Brune B, Cantoni O. Free Radic Biol Med. 2005 Dec 15;39(12):1638-49. Epub 2005 Sep 7.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10