Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Scapigliati Giuseppe Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Scapigliati Giuseppe
Qualifica
ASSOCIATE PROFESSOR
Facoltà
SCIENCE
Dipartimento
ENVIRONMENTAL SCIENCES
Settore Scientifico Disciplinare BIO/05
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PERSONALE STRUTTURATO

Cognome e Nome
FRANCESCO BUONOCORE
Qualifica
RESEARCHER
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza UNIVERSITY OF TUSCIA
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome
DANIELA CASANI
Qualifica
POST-DOC
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza
UNIVERSITY OF TUSCIA
Cognome e Nome
ELISA RANDELLI
Qualifica POST-DOC
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza
UNIVERSITY OF TUSCIA
Cognome e Nome GIANPAOLO ZARLETTI
Qualifica
PHD STUDENT
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza
UNIVERSITY OF TUSCIA
Cognome e Nome MARIA LUISA MORO
Qualifica
PHD STUDENT
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza
UNIVERSITY OF TUSCIA
Cognome e Nome
SABRINA MELONI
Qualifica POST-DOC
Dipartimento
ENVIRONMENTAL SCIENCES
Ente di appartenenza
UNIVERSITY OF TUSCIA
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

Studies on evolution of immune defence reactions, with special attention to biotechnologically interesting animals (aquacultured teleosts fishes).

Cloning of cytokine genes and immunoregulatory molecules of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata).

Expression of recombinant cytokines and studies of their bioactivity "in vivo" and "in vitro".

Production of mono and polyclonal antibodies against cytokines and immunoregulatory molecules.

Studies of immune molecules gene expression in treated and/or vaccined animals.

EST libraries construction from animals treated with bacterial and viral pathogens.

Determination of constitutive and induced gene expression using PCR, Real Time PCR, PCR-ARRAY system, and hybridization "in situ".

Determination of leucocytes and lymphocytes activity "in vitro" from control and treated animals, using cell proliferations and activation of intracellular second messenger pathways.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Mono and bidimensional electrophoresis, western blotting.
  • Production of monoclonal and policlonal antibodies. Animals manipulation.
  • Primary cell colture and continuous cell lines of omeothermic and eterothermic species.
  • Labelling and use of monoclonal and polyclonal antibodies, methabolic labelling of cells.
  • Nucleic Acid extraction from cells and tissues.
  • Cloning of genes. cDNA, genomic and expression libraries construction.
  • Expression of recombinant protein in bacterial and eucaryotic cells.
  • PCR and Real time PCR
  • EST construction
  • Microarray screening (in learning)

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
REAL TIME PCR
dept of Environmental Sciences
Francesco Buonocore
Luminometer Fluorimeter
dept of Environmental Sciences
Giuseppe Scapigliati
Cytofluorimeter
dept of Environmental Sciences
Giuseppe Scapigliati

PUBBLICAZIONI

Randelli E., Buonocore F. and Scapigliati G. Cell markers and determinants in fish immunology. Fish Shellfish Immunol., 25: 326-340, 2008.

Buonocore F., Forlenza M., Secombes C.J., Zou J., Mazzini M. and Scapigliati G. 2002. Expression in E. coli and purification of sea bass (Dicentrarchus labrax) interleukin-1beta, a possible immuno-adjuvant in aquaculture. Mar. Biotecnol., 6: 53-59, 2004.

Benedetti S., Randelli E., Buonocore F., Zou J., Secombes C.J. and Scapigliati G. Evolution of cytokine response: IL-1β directly affects intracellular Ca2+ concentration of teleost fish leukocytes through a receptor-mediated mechanism. Cytokine, 34: 9-16, 2006.

Buonocore F., Randelli E., Casani D., Mazzini M., Cappuccio I., Secombes C.J. and Scapigliati G. cDNA cloning and expression analysis of a cyclooxygenase-2 from sea bass (Dicentrarchus labrax L.) after vaccination. Aquaculture, 245: 301-310, 2005.

Buonocore F., Forlenza M., Randelli E., Benedetti S., Bossù P, Meloni S., Secombes C.J., Mazzini M. and Scapigliati G. Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1. Marine Biotechnology, 7: 609-617, 2005.

Scapigliati G., Buonocore F., Mazzini M. Biological activity of cytokines: an evolutionary perspective. Curr. Pharmacol. Design, 12: 3071-3081, 2006.

Buonocore F., Libertini A., Prugnoli D., Mazzini M. and Scapigliati G. Production and characterization of a continuous embryonic cell line from sea bass (Dicentrarchus labrax L.) Marine Biotechnology, 8: 80-85, 2006.

Meloni S, Zarletti G, Benedetti S, Randelli E, Buonocore F, Scapigliati G. Cellular activities during a mixed leucocyte reaction in the teleost sea bass Dicentrarchus labrax. Fish Shellfish Immunol. 20: 739-749, 2006.

Buonocore F, Randelli E, Bird S, Secombes CJ, Costantini S, Facchiano A, Mazzini M, Scapigliati G. The CD8alpha from sea bass (Dicentrarchus labrax L.): Cloning, expression and 3D modelling. Fish Shellfish Immunol. 20: 637-646, 2006

Scapigliati G., Buonocore F., Randelli E., Casani D., Meloni S., Zarletti G., Tiberi M., Pietretti D., Boschi I., Manchado M., Martin-Antonio B., Jimenez-Cantizano R., Bovo G., Borghesan F., Lorenzen N., Einer-Jensen K., Adams S., Thompson K., Alonso C., Bejar J., Cano I., Borrego J.J., Alvarez M.C. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. Fish Shellfish Immunol., In press, 2010.

E. Randelli, F. Buonocore, D. Casani, A.M. Fausto, G. Scapigliati An “immunome” gene panel for transcriptomic analysis of immune defence activities in the teleost sea bass (Dicentrarchus labrax L.): a review Italian Journal of Zoology (2009), 76(2): 146-157.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Giuseppe Scapigliati
Genetics and Cell Biology
Giorgio Prantera
University of Tuscia
Francesco Buonocore
Biological and biochemical evolution
Laura Zucconi
University of Tuscia

CONGRESSI C.I.B.

Congressi Partecipazione
X Congresso Nazionale di Biotecnologie, Perugia
Si
6° Congresso Nazionale Biotecnologie, Padova.
Si
4° Congresso Nazionale Biotecnologie, Torino.
Si
5° Congresso Nazionale Biotecnologie, L’Aquila
Si
9th National Biotechnology Congress, Torino
Si
8th National Biotechnology Congress, Siena Si