Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Caruso Carla Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Caruso Carla
Qualifica Professore associato
Facoltà Sienze MM FF NN
Dipartimento Agrobiologia e Agrochimica
Settore Scientifico Disciplinare BIO/10
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PERSONALE STRUTTURATO

Cognome e Nome Caporale Carlo
Qualifica Professore associato
Dipartimento
Dipartimento di Agrobiologia e Agrochimica
Ente di appartenenza
Università della Tuscia
Cognome e Nome Caruso Carla
Qualifica Professore associato
Dipartimento
Dipartimento di Agrobiologia e Agrochimica
Ente di appartenenza
Università della Tuscia
Cognome e Nome Chilosi Gabriele
Qualifica Professore associato
Dipartimento Dipartimento di Protezione delle Piante
Ente di appartenenza
Università della Tuscia
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
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Dipartimento
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Dipartimento
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PERSONALE NON STRUTTURATO

Cognome e Nome
Bertini Laura
Qualifica Assegnista di ricerca
Dipartimento
Dipartimento di Agrobiologia e Agrochimica
Ente di appartenenza
Università della Tuscia
Cognome e Nome
Proietti Silvia
Qualifica Studentessa
Dipartimento
Dipartimento di Agrobiologia e Agrochimica
Ente di appartenenza
Università della Tuscia
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
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Dipartimento
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Dipartimento
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Dipartimento
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LINEE DI RICERCA

Plant ability to cope with environmental stresses is essential for their survival. Following infection by pathogenic micro-organisms, plants rapidly activate local and systemic response leading to the synthesis of several antimicrobial molecules. Among them are antifungal proteins collectively known as “Pathogenesis Related (PR) proteins. The wide-ranging modes of action of PR proteins make them an ideal tool in defence strategies against “all comers”. In fact, in some cases overexpression of some PR genes has been shown to provide protection against several pathogen. This holds promises for the development of new strategies complementing conventional breeding programs to produce more resistant cultivars, thus meeting both the growing concern about the environment and the strong motivation to lower production costs. However, it is timely to highlight that overexpression of PR genes has been mainly obtained using the 35S cauliflower mosaic virus (CaMV) promoter, which confers constitutive expression of the transgene. A worthwhile research objective will involve the use of inducible and/or tissue-specific promoters that will allow expression of transgenic PR proteins only when needed and only in the desired tissues.Structural-functional characterization of wheat PR proteins and genes is only at the beginning and represents an interesting research area due to the relevance of this cereal for nutrition. The main aim of this research unit is to study in depth the mode of action of wheat PR-4 proteins and genes as well as the mechanism of antifungal activity towards pathogenic fungi and oomyces. Moreover, the isolation of genomic clones encoding these proteins will allow the study of PR4 gene organization and expression besides the identification of regulatory elements through traditional promoter deletion analysis coupled with mutagenesis of functional domain. Finally, recombinant vectors will be constructed carrying GUS (or GFP) reporter gene under control of wheat PR-4 promoter and used in expression analysis in transgenic plants challenged with different biotic and abiotic inducers.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Chromatographic and electrophoretic analyses
  • Gene cloning and expression
  • Recombinant protein production in prokariotic and eukariotic systems
  • Western, Northern, Southern and South-Western analysis
  • PCR and RT-PCR (both conventional and quantitative)
  • Functional proteomics: protein-protein interactions
  • Gene gun plant transformation
  • Agrobacterium tumefaciens-mediated plant transformation
  • Fungal disease assessment
  • Antifungal activity assay

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Spectrophotometer
Dipartimento di Agrobiologia e Agrochimica
Prof. Caruso
HPLC
Dipartimento di Agrobiologia e Agrochimica
Prof. Caruso
AKTA purifier
Dipartimento di Agrobiologia e Agrochimica
Prof. Luna
Light cycler
Dipartimento di Scienze Ambientali
Prof. Scapigliati
DNA sequencer
Dipartimento di Agrobiologia e Agrochimica
Dr. Delre
Climatic chamber
Dipartimento di Protezione delle Piante
Prof. Chilosi

PUBBLICAZIONI

Bertini L., Leonardi L., Caporale C., Tucci M., Cascone N., Di Berardino I., Buonocore V. and CARUSO C. Pathogen-responsive wheat PR4 genes are induced by plant activator of systemic acquired resistance and wounding. Plant Science, 2003, 164/6, 1067-1078.

Caporale C., CARUSO C., Colonna G., Facchiano A., Ferranti P., Metafora S., and Stiuso P. Structural properties of the protein SV-IV. European Journal of Biochemistry, 2003, 271, 263-271.

Caporale C., Di Berardino I., Leonardi L., Bertini L., Cascone A., Buonocore V. and CARUSO C. Wheat pathogenesis-related proteins of class 4 have ribonuclease activity. FEBS Letters, 2004, Vol 575 (1-3), 71-76.

Caporale C., Bertini L. Pucci P., Buonocore V. and CARUSO C. CysMap and CysJoin: database and tools for protein disulphides localisation. FEBS Letters, 2005, Vol 579 (14), 3048-3054.

Bertini L. and CARUSO C. Pathogenesis related genes as leading actors of plant defence mechanisms. In: “Plant Molecules: basic and applied research", Tiezzi A. and Santucci A. Eds., Gangemi Editore, 2006, 139-161

Fiocchetti F., CARUSO C., Bertini L , Vitti D., Saccardo F. and Tucci M. Over-expression of a pathogenesis-related gene in transgenic tomato interacts with the transcription pattern of other defence genes. J. Hort. Science & Biotech, 2006, 81 (1), 27-32.

Bertini L., Cascone A., Tucci M., D’Amore R., Di Berardino I., Buonocore V., Caporale C. and CARUSO C. Molecular and functional analysis of new members of the wheat PR4 gene family. Biol. Chem., 2006, 387, 1101-1111.

Roberti R., Veronesi AR., Cesari A., Cascone A, Di Berardino I., Bertini L. and CARUSO C. Induction of PR proteins and resistance by the biocontrol agent Clonostachys rosea in wheat plants infected with Fusarium culmorum, Plant Science, 2008, 175, 339-347.

Bertini L., Proietti S., Caporale C, and CARUSO C. Molecular characterization of a wheat protein induced by vernalisation. Protein J. 2009, 28, 253-262. DOI 10.1007/s10930-009-9190-3.

Bertini L., Caporale C., Testa M., Proietti S. and CARUSO C. Structural basis of the antifungal activity of wheat PR4 proteins. FEBS Letters, 2009, 583, 2865-2871.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Caruso
Genetica e biologia Cellulare
Luigi Bosco
Viterbo
Caporale
Genetica e biologia Cellular
Giorgio Prantera
Viterbo
Chilosi
Protezione delle Piante
Naldo Anselmi
Viterbo

CONGRESSI C.I.B.

Congressi Partecipazione
CNB3 Si
CNB4
Si
CNB5

CNB6
Si
CNB7
Si
CNB8

CNB9
CNB10 Si