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Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Pollegioni Loredano Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome
POLLEGGIONI LOREDANO
Qualifica FULL PROFESSOR
Facoltà FACULTY OF SCIENCES VARESE
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Settore Scientifico Disciplinare BIO/10
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PERSONALE STRUTTURATO

Cognome e Nome GIANLUCA MOLLA
Qualifica RESEARCH FELLOW, RU
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome
LUCIANO PIUBELLI
Qualifica RESEARCH FELLOW, RU
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome
SACCHI SILVIA
Qualifica RESEARCH FELLOW, RU
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome CALDINELLI LAURA
Qualifica RESEARCH TECHNICIAN
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA

PERSONALE NON STRUTTURATO

Cognome e Nome
ELENA ROSINI
Qualifica
POST-DOC
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
CAPPELLETTI PAMELA
Qualifica
POST-DOC
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
TONIN FABIO
Qualifica
PhD STUDENT
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome MELIS ROBERTA
Qualifica
PhD STUDENT
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND LIFE SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA

LINEE DI RICERCA

The research activity of this U.O. is mainly focused on:

1) protein engineering applied to industrial enzymology and to medical biotechnology,

2) the investigation of the life basis of the human pathologies,

3) elucidation of the role of D-amino acids on neurotransmission.

The wide experience acquired in the past years in resolving, identifying, and characterising proteins, introduced our research group in the functional post-genomics and protein engineering fields. At present the laboratory is engaged in the cloning and characterisation of proteins involved in human pathologies and production of enzymes relevant for biotechnological applications with novel functional properties.

The main goals of our biotechnological projects are:

- Cloning and overexpression in microorganisms of genes encoding for enzymes used in industrial applications.

- Protein engineering of flavoenzymes to produce mutants for specific biotechnological applications using two different approaches: rational design (by site directed mutagenesis) and directed evolution (by random mutagenesis, screening and mutants selection).

- Production and purification of recombinant proteins or native proteins, downstream processing and scaling-up studies.

- Covalent immobilization of enzymes on different matrixes to be employed in bioreactors.

- Set-up of bioconversion processes to produce fine chemicals.

- Development of biosensors to detect and quantify D-amino acids in food and biological samples.

- Gene therapy of solid tumors using D-amino acid oxidase from R. gracilis as prodrug converter (oxidative stress therapy mediated by hydrogen peroxide).

– Investigation of the life basis of schizophrenia (modulation of D-serine concentration in human brain).

- Investigation of the role of D-serine and D-aspartate on brain functions (and pathologies).


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Industrial enzymology
  • Protein engineering (SDM and directed evolution)
  • Protein expression, purification and downstream processing
  • Enzyme immobilization techniques
  • Immunolocalization
  • Transient kinetics techniques, protein-protein interactions
  • Neurotransmission
  • Fermentation technologies, cell cultures
  • Bioreactors and bioconversions optimisation
  • Bioinformatics and molecular modelling

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Stopped-flow spectrofluorimeter
DBSV
G. Molla
Modulable fermentor (2 & 10 l)
DBSV
G. Molla
Äkta chromatography systems
DBSV
L. Piubelli
epMotion 5075 liquid handler
DBSV
E. Rosini
HPLC Jasco
DBSV
L. Caldinelli
Sorvall Centrifuge
DBSV
L. Caldinelli
Beckman Centrifuge
DBSV
L. Caldinelli
UV-Vis Jasco Spectrophotometers
DBSV
L. Piubelli
CD Spectrophotometers Jasco J-815
DBSV
L. Piubelli

PUBBLICAZIONI

Pollegioni L, Molla G.2011 New biotech applications from evolved D-amino acid oxidases. Trends Biotechnol. 29(6):276-283.

Papouin T, Ladépêche L, Ruel J, Sacchi S, Labasque M, Hanini M, Groc L, Pollegioni L, Mothet JP, Oliet SH. Synaptic and Extrasynaptic NMDA Receptors Are Gated by Different Endogenous Coagonists. Cell. 2012 150(3):633-46.

Caldinelli L, Sacchi S, Molla G, Nardini M, Pollegioni L.Characterization of human DAAO variants potentially related to an increased risk of schizophrenia. Biochim Biophys Acta Mol. Basis Diseases. 2013;1832(3):400-10.

Golden E, Paterson R, Tie WJ, Anandan A, Flematti G, Molla G, Rosini E, Pollegioni L, Vrielink A. Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity. Biochem J. 2013 451(2):217-26.

Li Y, Sacchi S, Pollegioni L, Basu AC, Coyle JT, Bolshakov VY. Identity of endogenous NMDAR glycine site agonist in amygdala is determined by synaptic activity level. Nat Commun. 2013 4:1760

Hopkins SC, Heffernan ML, Saraswat LD, Bowen CA, Melnick L, Hardy LW, Orsini MA, Allen MS, Koch P, Spear KL, Foglesong RJ, Soukri M, Chytil M, Fang QK, Jones SW, Varney MA, Panatier A, Oliet SH, Pollegioni L, Piubelli L, Molla G, Nardini M, Large TH. Structural, Kinetic, and Pharmacodynamic Mechanisms of D-Amino Acid Oxidase Inhibition by Small Molecules. J Med Chem. 2013 56(9):3710-24.

Rosini E, Piubelli L, Molla G, Frattini L, Valentino M, Varriale A, D'Auria S, Pollegioni L. Novel biosensors based on optimized glycine oxidase. 2014 FEBS J. 281(15):3460-

Le Bail M, Martineau M, Sacchi S, Yatsenko N, Radzishevsky I, Conrod S, Ait Ouares K, Wolosker H,Pollegioni L, Billard JM, Mothet JP.Identity of the NMDA receptor coagonist is synapse specific and developmentally regulated in the hippocampus. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E204-13.

Molla G, Nardini M, Motta P, D'Arrigo P, Panzeri W, Pollegioni L. Aminoacetone oxidase from Streptococcus oligofermentans belongs to a new three-domain family of bacterial flavoproteins. Biochem J. 2014, 464(3):387-99.

Pollegioni L, Tonin F, Rosini E. Lignin-degrading enzymes. FEBS J. 2015 282(7):1190-213.


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Loredano Pollegioni
Biotecnologie, Bioscienze e Tecnologie Chirurgiche
Loredano Pollegioni
Varese - DBSV
Luciano Piubelli
Biotecnologie, Bioscienze e Tecnologie Chirurgiche
Loredano Pollegioni
Varese - DBSV
Gianluca Molla Biotecnologie, Bioscienze e Tecnologie Chirurgiche Loredano Pollegioni Varese - DBSV
Silvia Sacchi Biotecnologie, Bioscienze e Tecnologie Chirurgiche Loredano Pollegioni Varese - DBSV

CONGRESSI C.I.B.

Congressi Partecipazione
CNB5 Silvia Sacchi
Si
CNB6 Silvia Sacchi, Elena Rosini
Si
CNB7 Silvia Sacchi
Si
CNB8 Laura Caldinelli, Elena Rosini
Si
CNB9 Laura Caldinelli Si
CNB10 Loredano Polleggioni SI
CNB11 Loredano Pollegioni, Luciano Piubelli, Silvia Sacchi, Gianluca Molla, Laura Caldinelli, Pamela Cappelletti SI