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Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

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Pollegioni Loredano Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome
Pollegioni Loredano
Qualifica FULL PROFESSOR
Facoltà FACULTY OF SCIENCES VARESE
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Settore Scientifico Disciplinare BIO/10
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PERSONALE STRUTTURATO

Cognome e Nome GIANLUCA MOLLA
Qualifica RESEARCH FELLOW, RU
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome
LUCIANO PIUBELLI
Qualifica RESEARCH FELLOW, RU
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome
POLLEGIONI LOREDANO
Qualifica FULL PROFESSOR
Dipartimento DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza UNIVERSITY OF INSUBRIA
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome
ELENA ROSINI
Qualifica
POST-DOC
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
FEDERICA VOLONTE’
Qualifica
PhD STUDENT
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
LAURA CALDINELLI
Qualifica
POST-DOC
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome MATTIA PEDOTTI
Qualifica
PhD STUDENT
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
SILVIA SACCHI
Qualifica
POST-DOC
Dipartimento
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
Ente di appartenenza
UNIVERSITY OF INSUBRIA
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

The research activity of this U.O. is mainly focused on: 1) protein engineering applied to industrial enzymology and to medical biotechnology, 2) the investigation of the molecular basis of the human pathologies. The wide experience acquired in the past years in resolving, identifying, and characterising proteins, introduced our research group in the functional post-genomics and protein engineering fields. At present the laboratory is engaged in the cloning and characterisation of proteins involved in human pathologies and production of enzymes relevant for biotechnological applications with novel functional properties. The main goals of our biotechnological projects are: - Cloning and overexpression in microrganisms of genes encoding for enzymes used in industrial applications (such as Cephalosporin C acylase). - Protein engineering of flavoenzymes to produce mutants for specific biotechnological applications using two different approaches: rational design (by site directed mutagenesis) and directed evolution (by random mutagenesis, screening and mutants selection). - Production and purification of recombinant proteins or native proteins, downstream processing and scaling-up studies. - Covalent immobilization of enzymes (D-amino acid oxidase, Cephalosporin C acylase, cholesterol oxidase) on different matrixes to be employed in bioreactors. - Set-up of bioconversion processes (with flavoenzymes, proteases and acylases) to produce fine chemicals. - Development of biosensors to detect and quantify D-amino acids in food and biological samples. - Gene therapy of solid tumors using D-amino acid oxidase from R. gracilis as produg converter (oxidative stress therapy mediated by hydrogen peroxide). – Investigation of the molecular basis of schizophrenia (modulation of D-serine concentration in human brain).

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Industrial enzymology
  • Protein engineering (SDM and directed evolution)
  • Protein expression, purification and downstream processing
  • Enzyme immobilization techniques
  • Immunolocalization
  • Transient kinetics techniques, protein-protein interactions
  • Molecular biology
  • Fermentation technologies, cell cultures
  • Bioreactors and bioconversions optimisation
  • Bioinformatics and molecular modelling

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Stopped-flow spectrofluorimeter
DBSM
G. Molla
Modulable fermentor (2 & 10 l)
DBSM
G. Molla
Äkta chromatography systems
DBSM
L. Piubelli
Procise protein sequencer- Applied Biosystem
DBSM
A. Boselli
HPLC Waters and Jasco
DBSM
A. Boselli
Sorvall Centrifuge
DBSM
L. Pollegioni
Beckman Centrifuge
DBSM
L. Pollegioni
UV-Vis Jasco Spectrophotometers
DBSM
L. Piubelli
CD Spectrophotometers Jasco J-815
DBSM
L. Piubelli

PUBBLICAZIONI

1: Caldinelli L, Iametti S, Barbiroli A, Fessas D, Bonomi F, Piubelli L, Molla G, Pollegioni L. Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD.Protein Sci. 2008, 17(3):409-19.
2: Pollegioni L, Piubelli L, Sacchi S, Pilone MS, Molla G. Physiological functions of D-amino acid oxidases: from yeast to humans. Cell Mol Life Sci. 2007, 64(11):1373-94.
3: Panatier A, Theodosis DT, Mothet JP, Touquet B, Pollegioni L, Poulain DA, Oliet SH. Glia-derived D-serine controls NMDA receptor activity and synaptic memory. Cell. 2006, 125(4):775-84.
4: Pollegioni L, Lorenzi S, Rosini E, Marcone GL, Molla G, Verga R, Cabri W, Pilone MS. Evolution of an acylase active on cephalosporin C. Protein Sci. 2005, 14(12):3064-76.
5: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Fessas D, Molla G, Pilone MS, Pollegioni L. Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin. J Biol Chem. 2005, 280(24):22572-81. Epub 2005 Apr 7.
6: Mothet JP, Pollegioni L, Ouanounou G, Martineau M, Fossier P, Baux G. Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine. Proc Natl Acad Sci U S A. 2005, 102(15):5606-11.
7: Mörtl M, Diederichs K, Welte W, Molla G, Motteran L, Andriolo G, Pilone MS, Pollegioni L. Structure-function correlation in glycine oxidase from Bacillus subtilis. J Biol Chem. 2004, 279(28):29718-27.
8: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Piubelli L, Ferranti P, Picariello G, Pilone MS, Pollegioni L. Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase. J Biol Chem. 2004, 279(27):28426-34.
9: Pollegioni L, Diederichs K, Molla G, Umhau S, Welte W, Ghisla S, Pilone MS. Yeast D-amino acid oxidase: structural basis of its catalytic properties. J Mol Biol. 2002, 324(3):535-46.
10: Umhau S, Pollegioni L, Molla G, Diederichs K, Welte W, Pilone MS, Ghisla S. The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation. Proc Natl Acad Sci U S A. 2000, 97(23):12463-8.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Loredano Pollegioni
Biotecnologie
Giancarlo DeLuca
Varese - DSBSC
Luciano Piubelli
Biologia Cellulare e Molecolare
Magda deEguileor
Varese - DSBSC

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5 Silvia Sacchi
Si
CNB6 Silvia Sacchi, Elena Rosini
Si
CNB7 Silvia Sacchi
Si
CNB8 Laura Caldinelli, Elena Rosini
Si
CNB9 Laura Caldinelli Si
CNB10