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RESPONSABILE DELLA U. O. |
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Cognome e Nome |
Pollegioni Loredano
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Qualifica |
FULL PROFESSOR |
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Facoltà |
FACULTY OF SCIENCES VARESE |
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES |
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Settore Scientifico Disciplinare |
BIO/10 |
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PERSONALE STRUTTURATO |
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Cognome e Nome |
GIANLUCA MOLLA |
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Qualifica |
RESEARCH FELLOW, RU |
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES |
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA |
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Cognome e Nome |
LUCIANO PIUBELLI
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Qualifica |
RESEARCH FELLOW, RU |
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES |
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA |
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Cognome e Nome |
POLLEGIONI LOREDANO
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Qualifica |
FULL PROFESSOR |
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES |
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA |
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PERSONALE NON STRUTTURATO |
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Cognome e Nome |
ELENA ROSINI
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Qualifica |
POST-DOC
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA
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Cognome e Nome |
FEDERICA VOLONTE’
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Qualifica |
PhD STUDENT
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA
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Cognome e Nome |
LAURA CALDINELLI
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Qualifica |
POST-DOC
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA
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Cognome e Nome |
MATTIA PEDOTTI |
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Qualifica |
PhD STUDENT
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA
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Cognome e Nome |
SILVIA SACCHI
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Qualifica |
POST-DOC
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Dipartimento |
DEPARTMENT OF BIOTECHNOLOGY AND MOLECULAR SCIENCES
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Ente di appartenenza |
UNIVERSITY OF INSUBRIA
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Cognome e Nome |
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LINEE DI RICERCA |
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The research activity of this U.O. is mainly focused on: 1) protein engineering applied to industrial enzymology and to medical biotechnology, 2) the investigation of the molecular basis of the human pathologies. The wide experience acquired in the past years in resolving, identifying, and characterising proteins, introduced our research group in the functional post-genomics and protein engineering fields. At present the laboratory is engaged in the cloning and characterisation of proteins involved in human pathologies and production of enzymes relevant for biotechnological applications with novel functional properties. The main goals of our biotechnological projects are: - Cloning and overexpression in microrganisms of genes encoding for enzymes used in industrial applications (such as Cephalosporin C acylase). - Protein engineering of flavoenzymes to produce mutants for specific biotechnological applications using two different approaches: rational design (by site directed mutagenesis) and directed evolution (by random mutagenesis, screening and mutants selection). - Production and purification of recombinant proteins or native proteins, downstream processing and scaling-up studies. - Covalent immobilization of enzymes (D-amino acid oxidase, Cephalosporin C acylase, cholesterol oxidase) on different matrixes to be employed in bioreactors. - Set-up of bioconversion processes (with flavoenzymes, proteases and acylases) to produce fine chemicals. - Development of biosensors to detect and quantify D-amino acids in food and biological samples. - Gene therapy of solid tumors using D-amino acid oxidase from R. gracilis as produg converter (oxidative stress therapy mediated by hydrogen peroxide). – Investigation of the molecular basis of schizophrenia (modulation of D-serine concentration in human brain).
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TECNOLOGIE IN POSSESSO DELL'U. O. |
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- Protein engineering (SDM and directed evolution)
- Protein expression, purification and downstream processing
- Enzyme immobilization techniques
- Transient kinetics techniques, protein-protein interactions
- Fermentation technologies, cell cultures
- Bioreactors and bioconversions optimisation
- Bioinformatics and molecular modelling
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STRUMENTAZIONE |
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Denominazione
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Struttura ove la strumentazione è allocata
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Responsabile della strumentazione
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Stopped-flow spectrofluorimeter
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DBSM
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G. Molla
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Modulable fermentor (2 & 10 l)
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DBSM
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G. Molla
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Äkta chromatography systems
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DBSM
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L. Piubelli
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Procise protein sequencer- Applied Biosystem
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DBSM
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A. Boselli
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HPLC Waters and Jasco
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DBSM
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A. Boselli
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Sorvall Centrifuge
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DBSM
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L. Pollegioni
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Beckman Centrifuge
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DBSM
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L. Pollegioni
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UV-Vis Jasco Spectrophotometers
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DBSM
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L. Piubelli
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CD Spectrophotometers Jasco J-815
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DBSM
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L. Piubelli
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PUBBLICAZIONI |
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1: Caldinelli L, Iametti S, Barbiroli A, Fessas D, Bonomi F, Piubelli L, Molla G, Pollegioni L. Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD.Protein Sci. 2008, 17(3):409-19.
2: Pollegioni L, Piubelli L, Sacchi S, Pilone MS, Molla G. Physiological functions of D-amino acid oxidases: from yeast to humans. Cell Mol Life Sci. 2007, 64(11):1373-94.
3: Panatier A, Theodosis DT, Mothet JP, Touquet B, Pollegioni L, Poulain DA, Oliet SH. Glia-derived D-serine controls NMDA receptor activity and synaptic memory. Cell. 2006, 125(4):775-84.
4: Pollegioni L, Lorenzi S, Rosini E, Marcone GL, Molla G, Verga R, Cabri W, Pilone MS. Evolution of an acylase active on cephalosporin C. Protein Sci. 2005, 14(12):3064-76.
5: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Fessas D, Molla G, Pilone MS, Pollegioni L. Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin. J Biol Chem. 2005, 280(24):22572-81. Epub 2005 Apr 7.
6: Mothet JP, Pollegioni L, Ouanounou G, Martineau M, Fossier P, Baux G. Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine. Proc Natl Acad Sci U S A. 2005, 102(15):5606-11.
7: Mörtl M, Diederichs K, Welte W, Molla G, Motteran L, Andriolo G, Pilone MS, Pollegioni L. Structure-function correlation in glycine oxidase from Bacillus subtilis. J Biol Chem. 2004, 279(28):29718-27.
8: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Piubelli L, Ferranti P, Picariello G, Pilone MS, Pollegioni L. Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase. J Biol Chem. 2004, 279(27):28426-34.
9: Pollegioni L, Diederichs K, Molla G, Umhau S, Welte W, Ghisla S, Pilone MS. Yeast D-amino acid oxidase: structural basis of its catalytic properties. J Mol Biol. 2002, 324(3):535-46.
10: Umhau S, Pollegioni L, Molla G, Diederichs K, Welte W, Pilone MS, Ghisla S. The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation. Proc Natl Acad Sci U S A. 2000, 97(23):12463-8.
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DOTTORATI DI RICERCA |
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| Componente U.O. |
Dottorato di Ricerca |
Coordinatore |
Sede |
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Loredano Pollegioni
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Biotecnologie
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Giancarlo DeLuca
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Varese - DSBSC
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Luciano Piubelli
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Biologia Cellulare e Molecolare
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Magda deEguileor
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Varese - DSBSC
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CONGRESSI C.I.B. |
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| Congressi |
Partecipazione |
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CNB4
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CNB5 Silvia Sacchi
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Si |
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CNB6 Silvia Sacchi, Elena Rosini
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CNB7 Silvia Sacchi
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CNB8 Laura Caldinelli, Elena Rosini
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Si |
| CNB9 Laura Caldinelli |
Si |
| CNB10 |
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