Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Marinelli Flavia Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome
Marinelli Flavia
Qualifica
Full Professor
Facoltà Scienze, University of Insubria
Dipartimento
Biotechnology and Life Sciences (DBSV)
Settore Scientifico Disciplinare
CHIM/11
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PERSONALE STRUTTURATO

Cognome e Nome
Viviana Teresa Orlandi
Qualifica
Researcher
Dipartimento
Biotechnology and Life Sciences (DBSV)
Ente di appartenenza
Scienze, University of Insubria
Settore Scientifico Disciplinare BIO/19
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Cognome e Nome Fabrizio Bolognese
Qualifica Technician
Dipartimento Biotechnology and Life Sciences (DBSV)
Ente di appartenenza Scienze, University of Insubria
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PERSONALE NON STRUTTURATO

Cognome e Nome
Elisa Binda
Qualifica
Post-doc
Dipartimento
Biotechnology and Life Sciences (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome
Francesca Berini
Qualifica
Post-doc
Dipartimento
Biotechnology and Life Sciences (DBSV)
Ente di appartenenza
University of Insubria
Cognome e Nome Oleksandr Yushchuk
Qualifica Post-doc
Dipartimento Biotechnology and Life Sciences (DBSV)
Ente di appartenenza University of Insubria
Cognome e Nome Andres Andreo Vidal
Qualifica PhD Student
Dipartimento Biotechnology and Life Sciences (DBSV)
Ente di appartenenza University of Insubria
Cognome e Nome Eleonora Martegani
Qualifica PhD Student
Dipartimento Biotechnology and Life Sciences (DBSV)
Ente di appartenenza University of Insubria

LINEE DI RICERCA

The aim of the unit is to develop research projects mainly devoted to the biotechnological exploitation of uncommon and /or newly isolated and /or industrially relevant microorganisms, with a particular focus on antibiotics from actinomycetes. In parallel the research unit is involved in the optimization and process development for producing peptides and proteins by native or heterologous hosts, and  pigments from Pseudomonas spp, such as melanins, with possible applications for bioremediation, biofuel, pharmaceuticals and dermatological purposes.  Microbial products are still unsurpassed in their ability to provide novel and unpredictable molecular diversity able to face the concomitant evolution of pathogen resistant strategies and complexity of biological targets. It is commonly agreed that less than 1% of microbial species living on the Earth have been identified till now and probably much less can be easily cultivated and maintained in culture collections. By accessing to peculiar habitats (from Antarctic lake mats to Israeli desert sands) and by employing selective isolation protocols, we have isolated novel bacterial species belonging to biotechnologically relevant microbial groups. Novel metabolic pathways and products are been discovering in some of these organisms and their elucidation together with the development of cultivation methods and fermentation process represents a main interest of the lab. For two lipoglycopepetide antibiotics produced by two different actinomycetes, we are studying the self-resistance mechanisms, condition sine qua non for the antibiotic production and for its improvement. Interestingly the resistance genetic determinants and the phenotypes resemble and probably originated those described in the target pathogens, i.e. enterococci and staphylococci. For other recently discovered antibacterials, not only the chemical structure, but also the mechanism of action seems to be novel and promising for generating novel drugs. Novel species of actinomycetes have been also exploited as producers of novel enzymatic activities. Interest is focused on specific acylases and mannosidases able to modify complex antibiotic molecules or on hydrolytic activities for biomass recycling such as ligninases and chitinases. Additionally, bacterial pigments, such as melanins are being investigate since non-toxic, non-carcinogenic and biodegradable. Finally, we are developing  novel assays for testing the efficacy of novel antimicrobials on biofilms of model microorganisms such as Pseudomonas aeruginosa and Staphylococcus aureus.


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Batch and fed-batch fermentations
  • Isolation and cultivation of uncommon actinomycetes
  • Molecular biology and taxonomy of actinomycetes
  • Medium Optimization
  • Strain Improvement
  • Production of recombinant peptides and proteins
  • Identification of bioactive metabolites
  • Extraction and purification of bioactive products
  • Bacterila pigments as non cancerogenic compounds
  • Efficacy of novel antimicrobials on biofilms

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Three 3 liter Applikon fermenters fully equipped for batch and fed-batch fermentations
Microbial Biotecnology Lab
Flavia Marinelli
Bioexepert software for fermenter control and Stat graphics software for medium optimization
Microbial Biotecnology Lab
Flavia Marinelli
Shakers-Incubators
Microbial Biotecnology Lab
Flavia Marinelli
HPLC –DAD
Microbial Biotecnology Lab
Flavia Marinelli
LC-MS Spectrometry
Centro Grandi Attrezzature
Centro Grandi Attrezzature
RT-PCR
Centro Grandi Attrezzature
Centro Grandi Attrezzature
Electronic microscopes (SEM and TEM) Centro Grandi Attrezzature Centro Grandi Attrezzature

PUBBLICAZIONI


 

E.Binda , G.L.Marcone, L.Pollegioni, F.Marinelli (2012) Characterization of VanYn, a novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance in Nonomuraea sp. ATCC 3972. FEBS Journal. 279:3203-13

 

 

M. De Paola M, A.Mariani, P.Bigini, M.Peviani, G.Ferrara, M.Molteni, S.Gemma, P.Veglianese, V.Castellaneta, V.Boldrin, C.Rossetti, C.Chiabrando, G.Forloni, T.Mennini, R.Fanelli (2012) Neuroprotective effects of toll-like receptor 4 antagonism in spinal cord cultures and in a mouse model of motor neuron degeneration. Molecular  Medicine, 18:971-81

 

 

R.Oliver, K.J.Staples, J.Heckels, C.Rossetti, M.Molteni, M.Christodoulides (2012) Co-administration of the cyanobacterial lipopolysaccharide antagonist CyP with antibiotic inhibits cytokine production by an in vitro meningitis model infected with Neisseria meningitidis. Journal Antimicrobial Chemotherapy 67:1145-54

 

 

E.Binda, G.L.Marcone, F.Berini, L.Pollegioni, F.Marinelli (2013) Streptomyces spp. as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved in glycopeptides antibiotic resistance. BMC Biotechnology 13:24,

 

 

K.Hjort, I.Presti, A.Elväng, F.Marinelli, S.Sjöling (2014) Bacterial chitinase with phytopathogen control capacity from suppressive soil revealed by functional metagenomics. Applied Microbiology Biotechnology 98:2819-28

 

 

J.Treviño, C.Bayón, A.Ardá, F.Marinelli, R.Gandolfi, F.Molinari, J.Jimenez-Barbero, M.J.Hernáiz (2014) New insights into glycopeptide antibiotic binding to cell wall precursors using SPR and NMR spectroscopy Chemistry. A European Journal. 20:7363-72

 

 

L.Horbal, A.Kobylyanskyy, A.W.Truman, N.Zaburranyi, B.Ostash, A.Luzhetskyy, F.Marinelli, V.Fedorenko (2014) The pathway-specific regulatory genes, tei15* and tei16*, are the master switches of teicoplanin production in Actinoplanes teichomyceticus. Applied Microbiology Biotechnology 98:9295-309

 

 

G.L.Marcone, E.Binda, L.Carrano, M.Bibb M, F.Marinelli (2014) Relationship between glycopeptide production and resistance in the actinomycete Nonomuraea sp. ATCC 39727. Antimicrobial Agents and Chemotherapy. 58:5191-5201

 

 

V.Fedorenko, O.Genilloud, L.Horbal, G.L.Marcone, F.Marinelli, Y.Paitan, E. Z. Ron. (2015) Antibacterial discovery and development: from gene to product and back. BioMed Research International. Article ID 591349, 16

 

M.S.Cretoiu, F.Berini, A.M.Kielak, F.Marinelli, J.D.Van Elsas (2015) A novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil. Applied MicrobiologyBiotechnology, 99:8199-215

 

 

E. Binda, L. Carrano, G.L. Marcone, F. Marinelli. (2016). Extraction and Analysis of Peptidoglycan Cell Wall Precursors. Methods Mol Biol.1440:153-70.

 

 

C. Dalmastri, L. Gastaldo, G.L. Marcone, E. Binda, T. Congiu, F. Marinelli. (2016). Classification of Nonomuraea sp. ATCC 39727, an actinomycete that produces the glycopeptide antibiotic A40926, as Nonomuraea gerenzanensis sp. nov. Int J Syst Evol Microbiol. 66(2):912-21.

 

 

B. Rioseras, P. Yagüe, M.T. López-García, N. Gonzalez-Quiñonez, E. Binda, F. Marinelli, A. Manteca. (2016). Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor. Sci Rep. 6:21659.

 

 

F. Berini, S. Caccia, E. Franzetti, T. Congiu, F. Marinelli, M. Casartelli, G. Tettamanti. (2016). Effects of Trichoderma viride chitinases on the peritrophic matrix of Lepidoptera. Pest Manag Sci. 72(5):980-9.

 

 

C. Ferroni, G. Sotgiu, A. Sagnella, G. Varchi, A. Guerrini, D. Giuri, E. Polo, V.T. Orlandi, E. Marras, M. Gariboldi, E. Monti, A. Aluigi. (2016) Wool Keratin 3D Scaffolds with Light-Triggered Antimicrobial Activity. Biomacromolecules. 17(9):2882-90.

 

 

F. Bolognese, M. Bistoletti, P. Barbieri, V.T. Orlandi. (2016) Honey-sensitive Pseudomonas aeruginosa mutants are impaired in catalase A. Microbiology. 162(9):1554-1562.

 

 

F. Berini, C. Casciello, G.L. Marcone, F. Marinelli. (2017) Metagenomics: novel enzymes from nonculturable microbes. FEMS Microbiol Lett. 364(21)

 

 

G.L Marcone, E. Binda, M. Reguzzoni, L. Gastaldo, C. Dalmastri, F. Marinelli. (2017) Classification of Actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as Actinoplanes ramoplaninifer sp. nov. Int J Syst Evol Microbiol. 67(10):4181-4188.

 

 

L. Ausec, F. Berini, C. Casciello, M.S. Cretoiu, J.D. van Elsas, F. Marinelli, I. Mandic-Mulec. (2017). The first acidobacterial laccase-like multicopper oxidase revealed by metagenomics shows high salt and thermo-tolerance. Appl Microbiol Biotechnol. 101(15):6261-6276.

 

 

F. Berini, I. Presti, F. Beltrametti, M. Pedroli, K.J. Vårum, L. Pollegioni, S. Sjöling, F. Marinelli. (2017). Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome. Microb. Cell. Fact. 16:16.

 

 

V.T. Orlandi, F. Bolognese, E. Martegani, V. Cantaluppi, C. Medana, P. Barbieri. (2017) Response to photo-oxidative stress of Pseudomonas aeruginosa PAO1 mutants impaired in different functions. Microbiology. 163(11):1557-1567.

 

 

P. Cappelletti, E. Binda, M. Tunesi, D. Albani, C. Giordano, G. Molla, L. Pollegioni. (2017) Recombinant human Tat-Hsp70-2: A tool for neuroprotection. Protein Expr Purif. 138:18-24.

 

 

I. Armenia, G.L. Marcone, F. Berini, V.T. Olandi, C. Pirrone, E. Martegani, R. Gornati , G. Bernardini, F. Marinelli. (2018)Magnetic nanoconjugated teicoplanin: a novel tool for bacterial infection site targeting. Frontiers in Microbiology 9:2270

 

 

E. Binda, P. Cappelletti, F. Marinelli, G.L. Marcone. (2018) Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes. Antibiotics (Basel). 25(2).

 

 

G.L. Marcone, E. Binda, F. Berini, F. Marinelli. (2018) Old and new glycopeptide antibiotics: From product to gene and back in the post-genomic era. Biotechnol Adv. 36(2):534-554.

 

 

F. Berini, M. Verce, L. Ausec, E. Rosini, F. Tonin, L. Pollegioni, I. Mandic-Mulec. (2018). Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens. Appl Microbiol Biotechnol. 102(5):2425-2439

 

 

F. Berini, C. Katz, N. Gruzdev, M. Casartelli, G. Tettamanti, F. Marinelli. (2018) Microbial and viral chitinases: Attractive biopesticides for integrated pest management. Biotechnol Adv.36(3):818-838.

 

 

N. Baranzini, L. Monti, M. Vanott, V.T. Orlandi, F. Bolognese, D. Scaldaferri, R. Girardello, G. Tettamanti, M. de Eguileor, J. Vizioli, R. Taramelli, F. Acquati, A. Grimaldi. (2018) AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech. J Innate Immun. 26:1-18.

 

 

V.T. Orlandi, F. Bolognese, B. Rolando, S. Guglielmo, L. Lazzarato, R. Fruttero. (2018) Anti-Pseudomonas activity of 3-nitro-4-phenylfuroxan. Microbiology. 164(812):1557-1566.

 

 

V.T. Orlandi, E. Martegani, F. Bolognese. (2018) Catalase A is involved in the response to photooxidative stress in Pseudomonas aeruginosa. Photodiagnosis Photodyn Ther. 22: 233-240.

 

 

E. Caruso, S. Ferrara, P. Ferruti, A. Manfredi, E. Ranucci, V.T. Orlandi. (2018) Enhanced photoinduced antibacterial activity of a BODIPY photosensitizer in the presence of polyamidoamines. Lasers Med Sci. 33(6):1401-1407

 

 

V.T. Orlandi, F. Bolognese, P. Barbieri. (2018) Blue light enhances the antimicrobial activity of honey against Pseudomonas aeruginosa. Progress in Biomedical Optics and Imaging-Proceedings of SPIE. 10479-

 

 

A. Baj, E. Moro, M. Bistoletti, V.T. Orlandi, F. Crema, C. Giaroni. (2019) Glutamatergic Signaling Along The Microbiota-Gut-Brain Axis. Int J Mol Sci. 25;20(6).

 

 

O. Yushchuk, L. Horbal, B. Ostash, F. Marinelli, W. Wohlleben, E. Stegmann, V. Fedorenko. (2019) Regulation of teicoplanin biosynthesis: refining the roles of tei cluster-situated regulatory genes. Appl Microbiol Biotechnol. 2019 Apr 1. doi: 10.1007/s00253-019-09789-

 


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Flavia Marinelli
Scienze della Vita e Biotecnologie
Loredano Pollegioni
DBSV, Università dell’Insubria

CONGRESSI C.I.B.

Congressi Partecipazione

CNB11

Workshop "3rd Generation Biorefineries"

 

N. Baranzini, L. Monti, M. Vanott, V.T. Orlandi, F. Bolognese, D. Scaldaferri, R. Girardello, G. Tettamanti, M. de Eguileor, J. Vizioli, R. Taramelli, F. Acquati, A. Grimaldi. (2018) AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech. J Innate Immun. 26:1-18.

V.T. Orlandi, F. Bolognese, B. Rolando, S. Guglielmo, L. Lazzarato, R. Fruttero. (2018) Anti-Pseudomonas activity of 3-nitro-4-phenylfuroxan. Microbiology. 164(812):1557-1566.

V.T. Orlandi, E. Martegani, F. Bolognese. (2018) Catalase A is involved in the response to photooxidative stress in Pseudomonas aeruginosa. Photodiagnosis Photodyn Ther. 22: 233-240.

E. Caruso, S. Ferrara, P. Ferruti, A. Manfredi, E. Ranucci, V.T. Orlandi. (2018) Enhanced photoinduced antibacterial activity of a BODIPY photosensitizer in the presence of polyamidoamines. Lasers Med Sci. 33(6):1401-1407

V.T. Orlandi, F. Bolognese, P. Barbieri. (2018) Blue light enhances the antimicrobial activity of honey against Pseudomonas aeruginosa. Progress in Biomedical Optics and Imaging-Proceedings of SPIE. 10479-

A. Baj, E. Moro, M. Bistoletti, V.T. Orlandi, F. Crema, C. Giaroni. (2019) Glutamatergic Signaling Along The Microbiota-Gut-Brain Axis. Int J Mol Sci. 25;20(6).

O. Yushchuk, L. Horbal, B. Ostash, F. Marinelli, W. Wohlleben, E. Stegmann, V. Fedorenko. (2019) Regulation of teicoplanin biosynthesis: refining the roles of tei cluster-situated regulatory genes. Appl Microbiol Biotechnol. 2019 Apr 1. doi: 10.1007/s00253-019-09789-