Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
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Alberto Passi Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Alberto Passi
Qualifica
full professor
Dipartimento Department of Medicine and Surgery (DMC)
Settore Scientifico Disciplinare
Bio/10
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PERSONALE STRUTTURATO

Cognome e Nome Karousou Evgenia
Qualifica
PA
Dipartimento
Department of Medicine and Surgery (DMC)
Ente di appartenenza University of Insubria Varese
Cognome e Nome
Moretto Paola
Qualifica
Technician
Dipartimento
Department of Medicine and Surgery (DMC)
Ente di appartenenza University of Insubria Varese
Cognome e Nome
Pallotti Francesco
Qualifica
PA
Dipartimento
Department of Medicine and Surgery (DMC)
Ente di appartenenza University of Insubria Varese
Cognome e Nome Vigetti Davide
Qualifica
PA
Dipartimento
Department of Medicine and Surgery (DMC)
Ente di appartenenza University of Insubria Varese
Cognome e Nome
Viola Manuela
Qualifica
PA
Dipartimento
Department of Medicine and Surgery (DMC)
Ente di appartenenza University of Insubria Varese

PERSONALE NON STRUTTURATO

Cognome e Nome
Ilaria Caon
Qualifica
Post doc
Dipartimento Department of Medicine and Surgery (DMC)
Ente di appartenenza
University of Insubria Varese
Cognome e Nome Arianna Parnigoni
Qualifica PhD student
Dipartimento Department of Medicine and Surgery (DMC)
Ente di appartenenza
University of Insubria Varese

LINEE DI RICERCA

The main interest of the Passi’s group is the glycosaminoglycan (GAG) metabolism with particular attention to hyaluronic acid. GAGs are linear sulphated polysaccharides containing uronic acid and hexosamine residues. These molecules are very common in nature and linked to a protein core constitute the proteoglycan family. Hyaluronan (HA) is a special GAG without core protein and completely unsulphated. The aim of our work is the study of the mechanisms driving the GAG synthesis. In order to achieve this target we use a biochemical approach integrated with molecular biology techniques and morphology. To address the GAG chemistry we set up a new electrophoretic method based on the carbohydrate fluorescence (FACE). This method is very simple, fast and able to detect few pmoles of samples. Our preliminary data indicate that over expression in cells of UGDH, the enzyme involved in the synthesis of UDP-glucuronic acid (UDPGlcUA), the UDP uronic precursors of all GAGs, induced an increase in the cells of this precursor and consequently an increase of HA synthesis. On the other hand the silencing of the same enzyme by siRNA induced in the cells the opposite effect, confirming that the availability of UDPGlcUA controls the HA synthesis. In order to quantify the UDP sugars precursors in the cells, we developed a method using HPLC able to separate and quantify all UDP sugars involved in GAG synthesis. Moreover, the altered UDP sugar concentration in the cells was able to alter the expression of the enzymes involved in the UDP sugars synthesis and even the enzymes involved in GAG synthesis. On this basis we are now elucidating the mechanisms involved in this control of enzymes of the pathway of GAG synthesis. For this purpose we also consider the post transcriptional modification of the enzymes using a proteomic approach based on bi-dimensional electrophoresis and mass spectrometry analysis coupled with a genomic approach base on the microarray chips and real time RT-PCR.




TECNOLOGIE IN POSSESSO DELL'U. O.

  • HPLC and carbohydrate
  • FACE electrophoresis for carbohydrate
  • Confocal Cell Fluorescence
  • Cell cultures
  • Transient transfection by nucleofection
  • Signal silencing using siRNA
  • Cell membrane impedance (ECIS).
  • Carbohydrate Mass spectrometry
  • Real time RT-PCR

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Real time PCR
Pad. Bassani
Passi
Ultracentrifuge
Pad. Bassani
Moretto
HPLC/FPLC with fluorescence detector
Pad. Bassani
Viola
Nucleofector and cell cultures
Pad. Bassani
Vigetti

Inverted Fluorescence microscopy

Pad. Bassani
Karousou
Multiwavelength Fluorescence plate reader Pad. Bassani Moretto

PUBBLICAZIONI


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Davide Vigetti
Scienze della Vita e Biotecnologie
Silvia Sacchi
Varese
Manuela Viola
Scienze della Vita e Biotecnologie
Silvia Sacchi
Varese

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10