Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Tongiorgi Enrico Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Tongiorgi Enrico
Qualifica
Ricercatore confermato
Facoltà Scienze MFN
Dipartimento Dipartimento di Biologia
Settore Scientifico Disciplinare Anatomia Comparata e Citologia BIO/06
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PERSONALE STRUTTURATO

Cognome e Nome Edomi Paolo
Qualifica
RU
Dipartimento
Scienze della Vita
Ente di appartenenza Università di Trieste
Cognome e Nome Florian Fiorella
Qualifica
RU
Dipartimento
Scienze della Vita
Ente di appartenenza Università di Trieste
Cognome e Nome Marzari Roberto
Qualifica PO
Dipartimento
Scienze della Vita
Ente di appartenenza Università di Trieste



























PERSONALE NON STRUTTURATO

Cognome e Nome Baj Gabriele
Qualifica Assegnista di Ricerca
Dipartimento Scienze della Vita
Ente di appartenenza
Università di Trieste

Cognome e Nome Sabrina Boscolo
Qualifica
Assegnista di Ricerca
Dipartimento Scienze della Vita
Ente di appartenenza
Università di Trieste
Cognome e Nome Luigi Dolcetti
Qualifica Borsista
Dipartimento Scienze della Vita
Ente di appartenenza Università di Trieste

LINEE DI RICERCA

1.Development of diagnostic tools for autoimmune neurological diseases

Autoimmunity is an important cause of disease affecting 3-5% of the human population. In particular, 58 different neurological diseases with autoimmune etiology have been described.  Although some factors causing these diseases have been identified, the etiology of most of autoimmune diseases remains obscure. Nevertheless, the antibody response to self antigens is considered indicative of an anti-self response.

 

Only in a few cases the antigen responsible for the rupture of the self-tolerance has been characterized. This is particularly important for the diagnosis, since when an autoantigen is identified as specific marker, diagnostic tests based on the purified antigen are used. In all other cases, an immunofluorescence test is performed on histological sections challenged with the patient’s serum but only limited information on the antigen is obtained.

 

Objectives of this UO is to develop approaches to describe the complexity of the humoral response in autoimmune diseases. The antigens identified are produced as recombinant proteins to develop new diagnostic assays. In addition we are creating an image data bank to characterize the different staining pattern produced by sera from neurological patients using semiautomated immunohistochemical analysis of the reactivity of sera on rat brain sections.

 

2.Neurotrophic Factors as biomarkers and drug-targets for neurological diseases

Brain-Derived Neurotrophic Factor (BDNF) is a growth and trophic factor that belongs to the family of related secretory proteins called neurotrophins. BDNF plays multifaceted and in part opposed functions in both development and maintenance of the nervous system. Indeed, BDNF promotes both cell survival and cell death, neuronal maturation including neurites and dendritic spines outgrowth, and has a prominent role in various forms of synaptic plasticity such as long-term potentiation and long-term depression (Casaccia-Bonnefil et al., 1999; Huang and Reichardt, 2001; Lu et al., 2005). BDNF has a complex biology as it is composed of 22 transcripts in rodents (34 in humans) each of which contain one of the 11 different exons of BDNF gene encoding the 5’untranslated and a common downstream eaxon containing the coding region and the 3’untranslated regions (UTRs) (Aid et al., 2007).

 

The need and importance is increasingly felt for the identification of specific compounds able to modulate BDNF, which can be used as drugs for the treatment of neurological and neuropsychiatric diseases or for the treatment of deleterious effects caused to the nervous system by abuse of illegal or legal drugs. It is therefore object of the present invention the development of a method of screening for BDNF translation modulators which allow the measurement of BDNF protein production. In particular it would be highly desirable to identify a screening method which would allow to determine the expression of all possible BDNF variants, and allow, at the same time, to obtain information on the final amounts of the BDNF protein produced in response to a specific drug.


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Phage display
  • Bacterial two-hybrid system
  • Recombinant proteins production
  • Two-D gel proteomic analysis
  • Medium-scale cell survival assay
  • Medium-scale bioluminescent assay
  • Medium-scale semi-automated immunohystochemical analysis
  • Confocal microscopy
  • Electron microscopy

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Confocal microscope
Dip. Scienze della Vita
E. Tongiorgi
Nikon E800 light microscope
Dip. Scienze della Vita
E. Tongiorgi
Nikon LE300 inverted light microscope with micromanipulators and chamber for live cell analysis
Dip. Scienze della Vita
E. Tongiorgi
DAKO automation hystological robot
Dip. Scienze della Vita
E. Tongiorgi
Two-D gel proteomic analysis system
Dip. Scienze della Vita
P. Edomi
Trasmission and Scanning Electron microscopes
Centro Microscopia di Ateneo
S. Soranzo

PUBBLICAZIONI

Marzari R., Sblattero, D., Florian F., Tongiorgi E., Not T., Tommasini A., Ventura A. and Bradbury A. (2001) Molecular dissection of the tissue transglutaminase autoantibody response in celiac disease. J. Immunol. 166:4170-4176.

Hadjivassiliou M., Boscolo S, Davies-Jones G.A.B., Grünewald R.A., Not T., Sanders D.S., Simpson J.E., Tongiorgi E., Williamson C.A. and Woodroofe M.N. (2002) The humoral response in the pathogenesis of gluten ataxia. Neurology 58:1221-1226

Chies R, Nobbio L, Edomi P, Schenone A, Schneider C, Brancolini C. “Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22” J Cell Sci, 116: 987-999 (2003)

Boscolo S., Baldas V., Gobbi G., Giordano L., Cioni G., Not T., Ventura A., Tongiorgi E. (2005) Anti-brain but not celiac disease antibodies in Landau-Kleffner Syndrome and related epilepsies. J.Neuroimmunol. 160: 228-232

Boscolo S, Baldas V, Cancelli I, Hadjivassiliou M, Ventura A and Tongiorgi E (2006) Detection of anti-brain serum antibodies using a semi-quantitative immunohistological method. J. Immunol. Methods 309:139-149.

Boscolo S and Tongiorgi E. (2007) Quantification of anti-neural antibodies in autoimmune neurological disorders. Expert Review of Clinical Immunology 3:949-973

Chiaruttini C, Vicario A, Baj G, Li Z, Braiuca P, Gardossi L, Baraban J and Tongiorgi E. (2009) The G196A (V66M) mutation in BDNF suppresses dendritic trafficking of BDNF mRNA by disrupting its interaction with Translin. Proc. Natl. Acad. Sci. 106:16481-16486

Baj G and Tongiorgi E. (2009) Neuroblastoma cell survival is supported by BDNF splice variants of the second promoter cluster. Journal of Cell Science 122:36-43.

Boscolo S, Sblattero D, Florian F, Baldas V, Not T, Ventura A, Marzari R and Tongiorgi E. (2010) Anti-transglutaminase antibodies cause ataxia in mice. PLOS-one 5(3):e9698

Carlino D, Leone E, Di Cola F, Baj G, Marin R, Dinelli G, Tongiorgi E*, De Vanna M*. (2011). Low serum truncated-BDNF isoform correlates with higher cognitive impairment in schizophrenia. J Psychiatr Res. 45(2):273-9. (*=equal last authorship)


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
E. Tongiorgi
Scuola di Dottorato in Neuroscienze e Scienze Cognitive
P.P. Battaglini
Dip. Scienze della Vita
P.Edomi
Scuola di Dottorato in Neuroscienze e Scienze Cognitive
P.P. Battaglini
Dip. Scienze della Vita
R. Marzari
Dottorato in Nanotecnologie
P.Tommasini
Dip. Fisica
F. Florian
Dottorato in Nanotecnologie
P.Tommasini
Dip. Fisica

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6
Si
CNB7
Si
CNB8

CNB9
CNB10