Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Gennaro Renato Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Gennaro Renato
Qualifica Professore Ordinario
Facoltà
Scienze Matematiche, Fisiche e Naturali
Dipartimento Biochimica Biofisica e Chimica delle Macromolecole
Settore Scientifico Disciplinare BIO/10 Biochimica
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PERSONALE STRUTTURATO

Cognome e Nome
Gennaro Renato
Qualifica Professore Ordinario
Dipartimento Biochimica Biofisica e Chimica delle Macromolecole
Ente di appartenenza Università di Trieste
Cognome e Nome
Scocchi Marco
Qualifica
Ricercatore Universitario
Dipartimento Biochimica Biofisica e Chimica delle Macromolecole
Ente di appartenenza Università di Trieste
Cognome e Nome
Tossi Alessandro
Qualifica Professore Associato
Dipartimento Biochimica Biofisica e Chimica delle Macromolecole
Ente di appartenenza Università di Trieste
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome
Antcheva Nikolika
Qualifica
Post-doc researcher
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome
Benincasa Monica
Qualifica
Post-doc researcher
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome Creatti Luisa
Qualifica
Dottoranda
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome
Floreano Paolo
Qualifica
Dottorando
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome Mattiuzzo Maura
Qualifica
Dottoranda
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome
Zlatev Ventsislav
Qualifica
Borsista
Dipartimento
Borsista
Ente di appartenenza
Università di Trieste
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

Antimicrobial peptides are powerful molecules from innate defence with multiple functions in immunity and a wide range of potential applications as anti-infective drugs. Understanding the antibiotic properties of these molecules is of increasing importance due to the alarming spread of antibiotic resistant nosocomial strains of bacteria and fungi, and the consequent pressing need to develop novel and effective classes of antimicrobial drugs.

Our studies cover the biological activity, mode of action, evolution and de novo design of different classes of antimicrobial peptides, with the aim to better understand their role in immunity, to identify/design potent, selective antimicrobial peptides, to clearly identify their molecular targets, and develop them as therapeutic agents

The main focus of our research is the molecular characterization of the antimicrobial peptides (AMPs) activity. Currently, our laboratory is pursuing a number of questions concerning the mechanism of action of different structural classes of animal-derived AMPs, including cathelicidins, defensins, histatins, and de novo designed artificial peptides. Understanding their function and how this has been shaped by evolution will allow a better assessment of their role in maintaining the organism free from infection and permit their development also as exogenously administered drugs. This implies i) understanding the mode of action of different types of AMPs and how this has been modulated in evolutionarly selected sequence/structure variations; ii) identifying the molecular targets for these molecules, at the membrane or cytoplasmic levels; iii) redesigning peptides or designing peptides de novo to probe structure/activity relationships; iv) determining how bacteria respond to their action, and in particular what resistance mechanisms they can develop and v) use all this information for the design of potentially useful therapeutics.
The group also has an interest in the computer assisted design, synthesis and evaluation of pseudopeptidic inhibitors of microbial and viral aspartic proteases.
Main topics:

i) Investigation of the mode of action of the cathelicidin-derived proline-rich antimicrobial peptides and identification of their molecular target(s);
ii) Genetic analysis of AMP-resistant-mutants to identify gene(s) involved in the bactericidal action of these peptides;
iii) Investigation of the cell-penetrating and drug delivery features of the proline-rich group of peptides;
iv) Investigation of the mode of antimicrobial action of the cathelicidin -helical peptides and of β-defensins, in relation to their molecular evolution in mammals;
v) Investigation of the immunomodulatory roles of the cathelicidin -helical peptides and of β-defensins;
vi) Biophysical studies on the interaction of cathelicidin -helical peptides and of β-defensins with model membranes;
vii) Structure-activity studies to improve the antimicrobial activity and target selectivity of AMPs
viii) In vitro activity of selected AMPs against antibiotic multi-resistant pathogens;
ix) Testing of AMP efficacy in vivo via mouse acute infection models;

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Solid-phase peptide synthesis
  • HPLC-grade peptide/protein purification
  • Ion spray mass spectrometry
  • Antimicrobial in vitro assays
  • Gene cloning
  • 2-D gel electrophoresis
  • Polyclonal antibody production facility

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
PioneerTM Peptide Synthesis System
Dip. Biochimica Biofisica e Chimica delle Macromolecole
U.O. R. Gennaro
HPLC ÄKTA Basic, Amersham Biotech
Dip. Biochimica Biofisica e Chimica delle Macromolecole
U.O. R. Gennaro
Apparato per 2-D gel electrophoresis
Dip. Biochimica Biofisica e Chimica delle Macromolecole
U.O. R. Gennaro
API-I ion spray mass spectrometer PE SCIEX
Dip. Biochimica Biofisica e Chimica delle Macromolecole
U.O. R. Gennaro
Spectropolarimeter Jasco J-600
Dip. Biochimica Biofisica e Chimica delle Macromolecole
U.O. R. Gennaro

PUBBLICAZIONI

1. Zelezetsky I, Pacor S, Pag U, Papo N, Shai Y, Sahl HG & Tossi A (2005) Controlled alteration of the shape and conformational stability of alpha-helical cell-lytic peptides: effect on mode of action and cell specificity. Biochem J 390, 177-188.
2. Wu W, Wieckowski S, Pastorin G, Benincasa M, Klumpp C, Briand JP, Gennaro R, Prato M & Bianco A (2005) Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes. Angew Chem Int Ed Engl 44, 6358-6362.
3. Scocchi M, Zelezetsky I, Benincasa M, Gennaro R, Mazzoli A & Tossi A (2005) Structural aspects and biological properties of the cathelicidin PMAP-36. Febs J 272, 4398-4406.
4. Sahl HG, Pag U, Bonness S, Wagner S, Antcheva N & Tossi A (2005) Mammalian defensins: structures and mechanism of antibiotic activity. J Leukoc Biol 77, 466-475.
5. Benincasa M, Scocchi M, Podda E, Skerlavaj B, Dolzani L & Gennaro R (2004) Antimicrobial activity of Bac7 fragments against drug-resistant clinical isolates. Peptides 25, 2055-2061.
6. Antcheva N, Boniotto M, Zelezetsky I, Pacor S, Verga Falzacappa MV, Crovella S & Tossi A (2004) Effects of positively selected sequence variations in human and Macaca fascicularis beta-defensins 2 on antimicrobial activity. Antimicrob Agents Chemother 48, 685-688.
7. Boniotto M, Antcheva N, Zelezetsky I, Tossi A, Palumbo V, Verga Falzacappa MV, Sgubin S, Braida L, Amoroso A & Crovella S (2003) A study of host defence peptide beta-defensin 3 in primates. Biochem J 374, 707-714.
8. Risso A, Braidot E, Sordano MC, Vianello A, Macri F, Skerlavaj B, Zanetti M, Gennaro R & Bernardi P (2002) BMAP-28, an antibiotic peptide of innate immunity, induces cell death through opening of the mitochondrial permeability transition pore. Mol Cell Biol 22, 1926-1935.
9. Gennaro R, Zanetti M, Benincasa M, Podda E & Miani M (2002) Pro-rich antimicrobial peptides from animals: structure, biological functions and mechanism of action. Curr Pharm Des 8, 763-778.
10. Skerlavaj B, Scocchi M, Gennaro R, Risso A & Zanetti M (2001) Structural and functional analysis of horse cathelicidin peptides. Antimicrob Agents Chemother 45, 715-722.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Renato Gennaro
Medicina Molecolare
Prof. G. del Sal
Univ. Trieste
Alessandro Tossi
Scienze Biomolecolari
Prof. F. Vittur
Univ. Trieste
Marco Scocchi
Scienze Biomolecolari
Prof. F. Vittur
Univ. Trieste

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10