Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

Login



Rosati Sergio Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome
Rosati Sergio
Qualifica PO (professor)
Facoltà Veterinari Medicine, Turin
Dipartimento Scienze Veterinarie
Settore Scientifico Disciplinare VET05
E-mail Questo indirizzo e-mail è protetto dallo spam bot. Abilita Javascript per vederlo.

PERSONALE STRUTTURATO

Cognome e Nome
Grego Elena
Qualifica
RU
Dipartimento Scienze Veterinarie
Ente di appartenenza
Università di Torino Facoltà di Medicina veterinaria
Cognome e Nome
Mannelli Alessandro
Qualifica
PA
Dipartimento Scienze Veterinarie
Ente di appartenenza
Università di Torino Facoltà di Medicina veterinaria
Cognome e Nome Nebbia Patrizia
Qualifica
PA
Dipartimento Scienze Veterinarie
Ente di appartenenza
Università di Torino Facoltà di Medicina veterinaria
Cognome e Nome Bertolotti Luigi
Qualifica PA
Dipartimento Scienze Veterinarie
Ente di appartenenza Università di Torino
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

Small ruminant retrovirus infection: isolation, antigenic and genetic characterization of viral isolates, viral genes expression in prokaryotic system; development of serological tests; correlation between endogenous and exogenous retroviral sequences in ovine pathology - Lyme borreliosis: development and application of recombinant subunit antigen for serological diagnosis in domestic and wild species - Paratuberculosis: characterization of M. paratuberculosis field isolates by arbitrarily primed polymerase chain reaction and AFLP- Characterization of ruminant Mycoplasma lipoproteins. Mutagenesis and recombinant antigens.

Bovine herpesvirus and pestivirus. Expression of viral genes in baculovirus system and development of diagnostic tests.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Prokaryotic and eukaryotic expression systems
  • Recombinant antigens
  • Epitope mapping
  • Imunoenzymatic assays
  • 2D-electrophoresis
  • Nucleic acid sequencing
  • Real time PCR
  • Multiplex PCR

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Abi Prism 310 Genetic analyser
Scienze Veterinarie Lab: MolBiol2
Margherita Profiti
FPLC Pharmacia
Scienze Veterinarie, Infectious diseases unit, Lab: Protein Analysis
Sergio Rosati
i-Cycler BioRad
Scienze Veterinarie, Infectious diseases unit, Lab: MolBiol2
Elena Grego

PUBBLICAZIONI

1. ROSATI S., ROBINO P, FADDA M, POZZI S, MANNELLI A, PITTAU M. (2000). Expression and antigenic characterization of recombinant Mycoplasma agalactiae P48 major surface protein. VETERINARY MICROBIOLOGY. vol. 71, pp. 201-210 ISSN: 0378-1135.
2. ROSATI S., PITTAU M, ALBERTI A, POZZI S, YORK D.F., SHARP J.M., PALMARINI M. (2000). An accessory open reading frame (orf-X) of jaagsiekte sheep retrovirus is conserved between different virus isolates. VIRUS RESEARCH. vol. 66, pp. 109-116 ISSN: 0168-1702.
3. GREGO E., PROFITI M., GIAMMARIOLI M., GIANNINO L., RUTILI D., WOODALL C., ROSATI S. (2002). Genetic heterogoneity of small ruminant lentiviruses involves immunodominant epitope of capsid antigen and affects sensitivity of signle-strain based immunoassay. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. vol. 9, pp. 828-832 ISSN: 1071-412X.
4. ROSATI S., ORTOFFI M., PROFITI M., MANNELLI A., MIGNONE W., BOLLO E., GRADONI L. (2003). Prokaryotic expression and antigenic characterization of three recombinant leishmania antigens for serological diagnosis of canine leishmaniasis. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. vol. 10, pp. 1153-1156 ISSN: 1071-412X.
5. MANELLI A., BOGGIATTOG., GREGO E., CINCO M., MURGIA R., STEFANELLI S., DE MENEGHI D., ROSATI S. (2003). Acarological risk of exposure to agents of tick-borne zoonoses in the first recognized Italian focus of Lyme borreliosis. EPIDEMIOLOGY AND INFECTION. vol. 131, pp. 1139-1147 ISSN: 0950-2688.
6. YAGHOUBA K., ROSATI S., PROFITI M., DIOP O., KABORET Y.Y., ALOGNINOUWA T., LENA P. (2004). Enzootic nasal tumour virus demonstrated in Senegal by direct detection of provirus from tumour DNA. VETERINARY RECORD. vol. 155, pp. 526-528 ISSN: 0042-4900.
7. ROSATI S., PROFITI M., LORENZETTI R., BANDECCHI P., MANNELLI A., ORTOFFI M., TOLARI F, CIABATTI I.M. (2004). Development of recombinant capsid antigen / transmembrane epitope fusion proteins for serological diagnosis of animal lentivirus infections. JOURNAL OF VIROLOGICAL METHODS. vol. 121, pp. 73-78 ISSN: 0166-0934.
8. ROSATI S., PROFITI M., GREGO E., CARROZZA M.L., MAZZEI M., BANDECCHI P. (2004). Antigenic variability of ovine lentivirus isolated in Italy. VETERINARY RESEARCH COMMUNICATIONS. vol. 28, pp. 319-322 ISSN: 0165-7380.
8. ROSATI S., PROFITI M., GREGO E., CARROZZA M.L., MAZZEI M., BANDECCHI P. (2004). Antigenic variability of ovine lentivirus isolated in Italy. VETERINARY RESEARCH COMMUNICATIONS. vol. 28, pp. 319-322 ISSN: 0165-7380.
9. GREGO E., BERTOLOTTI L., CARROZZA M.L., PROFITI M., ROSATI S. (2005). Genetic and antigenic characterization of the matrix protein of two genetically distinct ovine lentiviruses. VETERINARY MICROBIOLOGY. vol. 106, pp. 179-185 ISSN: 0378-1135.
10. BOARINO A., SCALONE A., GRADONI L., FERROGLIO E., VITALE F., ZANATTA R., GIUFFRIDA M. G., ROSATI S. (2005). Development of a recombinant chimeric antigen expressing immunodominant B epitopes of Leishmania infantum for the serodiagnosis of visceral leishmaniasis. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. vol. 12, pp. 647-653 ISSN: 1071-412X.
ROBINO P., ALBERTI A., PITTAU M., CHESSA B., MICILETTA M., NEBBIA P., LE GRAND D., ROSATI S. (2005). Genetic and antigenic characterization of the surface lipoprotein P48 of Mycoplasma bovis. VETERINARY MICROBIOLOGY. Vol. 109, pp. 201-209 ISSN: 0378-1135.

KANE Y., ROSATI S., DIOP O.M., PROFITI M., NIANG I., KADJA M., KABORET Y.Y., ALOGNINOUWA T., LENA P. (2005). Tissue targets and phylogenetic characteristic of the enzotic nasal tumour virus (ENTV) infecting sahelian sheep. REVUE DE MÉDECINE VÉTÉRINAIRE, vol. 156; p. 29-36, ISSN: 0035-1555

 

KITTELBERGER R, O'KEEFE JS, MEYNELL R, SEWELL M, ROSATI S., LAMBERT M, DUFOUR P, PEPIN M. (2006). Comparison of four diagnostic tests for the identification of serum antibodies in small ruminants infected with Mycoplasma agalactiae. NEW ZEALAND VETERINARY JOURNAL, vol. 54; p. 10-15, ISSN: 0048-0169

 

ALBERTI A., ADDIS M.F., CHESSA B., CUBEDDU T., PROFITI M., ROSATI S., RUIU A., PITTAU M. (2006). Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, vol. 18; p. 40-50, ISSN: 1040-6387

 

LACERENZA D, GIAMMARIOLI M, GREGO E, MARINI C, PROFITI M, RUTILI D, ROSATI S. (2006). Antibody response in sheep experimentally infected with different small ruminant lentivirus genotypes. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 112; p. 264-271, ISSN: 0165-2427

 

GREGO E, USLENGHI F, STRASSER M, LUZZAGO C, FRIGERIO M, PELETTO S, ROSATI S. (2007). Development and application of an enzyme-linked immunosorbent assay for detection of bovine viral diarrhea antibody based on Erns glycoprotein expressed in a baculovirus system. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, vol. 19(1); p. 21-27, ISSN: 1040-6387

 

ACUTIS PL, PELETTO S, GREGO E, COLUSSI S, RIINA MV, ROSATI S., MIGNONE W, CARAMELLI M (2007). Comparative analysis of the prion protein (PrP) gene in cetacean species. GENE, vol. 392; p. 230-238, ISSN: 0378-1119

 

G. AMORE, L. TOMASSONE, E. GREGO, C. RAGAGLI, L. BERTOLOTTI, P. NEBBIA, ROSATI S., A. MANNELLI (2007). Borrelia lusitaniae in immature Ixodes ricinus (Acari: Ixodidae) feeding on common wall lizards (Podarcis muralis) in Tuscany, central Italy. JOURNAL OF MEDICAL ENTOMOLOGY, vol. 44; p. 303-307, ISSN: 0022-2585

 

P. ROBINO, A. ALBERTI, B. CHESSA, M. PITTAU, M. PROFITI, ROSATI S. (2007). Molecular Cloning and Expression of a Surface Lipoprotein of Mycoplasma Capricolum as a Potential Antigen for Serological Diagnosis. VETERINARY RESEARCH COMMUNICATIONS, vol. 31(suppl. 1); p. 257-260, ISSN: 0165-7380

 

FRAISIER C, ARNARSON H, BARBEZANGE C, ANDRSDOTTIR V, CARROZZA M.L, DE ANDRES D, TOLARI F, ROSATI S., LUJAN L, PPIN M, AMORENA B, HARKISS G, BACKLAWS B, SUSAN-MONTI M (2007). Expression of the gp150 maedi visna virus envelope precursor protein by mammalian expression vectors. JOURNAL OF VIROLOGICAL METHODS, vol. 146; p. 363-367, ISSN: 0166-0934

 

REINA R, GLARIA I, BENAVIDES J, DE ANDRÉS X, CRESPO H, SOLANO C, PÉREZ V, LUJÁN L, PÉREZ M.M, PÉREZ J. DE LA LASTRA M, ROSATI S., BLACKLAWS B, HARKISS G, DE ANDRÉS D, AMORENA B (2007). Association of CD80 and CD86 Expression Levels with Disease Status of Visna/Maedi Virus Infected Sheep. VIRAL IMMUNOLOGY, vol. 20; p. 609-622, ISSN: 0882-8245

 

GREGO E, BERTOLOTTI L, QUASSO A, PROFITI M, LACERENZA D, MUZ D, ROSATI S. (2007). Genetic characterization of small ruminant lentivirus in Italian mixed flocks: evidence for a novel genotype circulating in a local goat population. JOURNAL OF GENERAL VIROLOGY, vol. 88; p. 3423-3427, ISSN: 0022-1317

 

A. ALBERTI, P. ROBINO, C. BERNARDO, ROSATI S., M. F. ADDIS, P. MERCIER, A. MANNELLI, T. CUBEDDU, M. PROFITI, E. BANDINO, R. THIERY, M. PITTAU (2008). Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA. VETERINARY MICROBIOLOGY, vol. 128; p. 81-89, ISSN: 0378-1135

 

D. LACERENZA, F. GENOVESE, M. PROFITI, D. NUCERA, ROSATI S. (2008). Characterization of an immunodominant epitope of small ruminant lentivirus (SRLV) nucleoprotein. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 125; p. 361-367, ISSN: 0165-2427

 

GREGO E, DE MENEGHI D, ALVAREZ V, BENITO AA, MINGUIJÓN E, ORTÍN A, MATTONI M, MORENO B, DE VILLARREAL MP, ALBERTI A, CAPUCCHIO MT, CAPORALE M, JUSTE R, ROSATI S., DE LAS HERAS M (2008). Colostrum and milk can transmit jaagsiekte retrovirus to lambs. VETERINARY MICROBIOLOGY, vol. 130; p. 247-257, ISSN: 0378-1135

 

FRANCO DAPRA, ALDO SCALONE, WALTER MIGNONE, EZIO FERROGLIO, ALESSANDRO MANNELLI, ALBERTO BIGLINO, RENATO ZANATTA, LUIGI GRADONI, ROSATI S. (2008). Validation of a recombinant based antibody ELISA for diagnosis of human and canine leishmaniasis. JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, vol. 29 (3); p. 244-256, ISSN: 1532-1819

 

REINA ARIAS R, BARBEZANGE C, NIESALLA H, DE ANDRÉS X, ARNARSON H, BIESCAS E, MAZZEI M, FRAISIER C, MCNEILLY TN, LIU C, PEREZ M, CARROZZA ML, BANDECCHI P, SOLANO C, CRESPO H, GLARIA I, HUARD C, SHAW DJ, DE BLAS I, DE ANDRÉS D, TOLARI F, ROSATI S., SUZAN-MONTI M, ANDRÉSDOTTIR V, TORSTEINSDOTTIR S, PETURSSON G, LUJAN L, PEPIN M, AMORENA B, BLACKLAWS B, HARKISS GD. (2008). Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes. VACCINE, vol. 26; p. 4494-4505, ISSN: 0264-410X

 

AIRAS REINA R, GREGO E, BERTOLOTTI L, DE MENEGHI D, ROSATI S. (2009). Genome analysis of small-ruminant lentivirus genotype E: a caprine lentivirus with natural deletions of the dUTPase subunit, vpr-like accessory gene, and 70-base-pair repeat of the U3 region. JOURNAL OF VIROLOGY, vol. 83; p. 1152-1155, ISSN: 0022-538X

 

CHESSA B., PITTAU M., PURICELLI M., ZOBBA R., CORADDUZZA E., DALL'ARA P., ROSATI S., POLI G., ALBERTI A. (2009). Genetic immunization with the immunodominant antigen P48 of Mycoplasma agalactiae stimulates a mixed adaptive immune response in BALBc mice. RESEARCH IN VETERINARY SCIENCE, vol. 86; p. 414-420, ISSN: 0034-5288

 

NIESALLA H., DE ANDRES X., BARBEZANGE C., FRAISIER C., REINA ARIAS R., ARNARSON H., BIESCAS E., MAZZEI M., MCNEILLY T.N., LIU C., WATKINS C., PEREZ M., CARROZZA M.L., BANDECCHI P., SOLANO C., CRESPO H., GLARIA I., HUARD C., SHAW D.J., DE BLAS I., DE ANDRES D., TOLARI F., ROSATI S., SUZAN-MONTI M., ANDRESDOTTIR V., TORSTEINSDOTTIR S., PETURSSON G., BADIOLA J., LUJAN L., PEPIN M., AMORENA B., BLACKLAWS B., HARKISS G.D. (2009). Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes. VACCINE, vol. 27; p. 260-269, ISSN: 0264-410X

 

DE ANDRÉS X, REINA R, CIRIZA J, CRESPO H, GLARIA I, RAMÍREZ H, GRILLÓ MJ, PÉREZ MM, ANDRÉSDÓTTIR V, ROSATI S., SUZAN-MONTI M, LUJÁN L, BLACKLAWS BA, HARKISS GD, DE ANDRÉS D, AMORENA B. (2009). Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus. VACCINE, vol. 27; p. 4591-4600, ISSN: 0264-410X

 

CARROZZA M.L., MAZZEI M., LACERENZA D., DEL CHIARO L., GIAMMARIOLI M., MARINI C., RUTILI D., ROSATI S., TOLARI F. (2009). Seroconversion against SU5 derived synthetic peptides in sheep experimentally infected with different SRLV genotypes. VETERINARY MICROBIOLOGY, vol. -137; p. -369--374, ISSN: 0378-1135

GREGO E, LACERENZA D, ARIAS RR, PROFITI M, ROSATI S. (2009). Serological characterization of the new genotype E of small ruminant lentivirus in Roccaverano goat flocks. VETERINARY RESEARCH COMMUNICATIONS, vol. 33; p. 137-140, ISSN: 1573-7446

REINA ARIAS R., GREGO E., PROFITI M., GLARIA I., ROBINO P., QUASSO A., AMORENA B., ROSATI S. (2009). Development of specific diagnostic test for small ruminant lentivirus genotype E. VETERINARY MICROBIOLOGY, vol. 138; p. 251-257, ISSN: 0378-1135

 

CARPI G., BERTOLOTTI L., ROSATI S., RIZZOLI A. (2009). Prevalence and genetic variability of tick-borne encephalitis virus in host-seeking Ixodes ricinus in northern Italy. JOURNAL OF GENERAL VIROLOGY, vol. 90; p. 2877-2883, ISSN: 0022-1317

 

JUGANARU M., REINA R., GREGO E., PROFITI M., ROSATI S. (2010). LTR promoter activity of SRLV genotype E, strani Roccaverano.  VETERINARY RESEARCH COMMUNICATIONS, vol. 34 (Suppl 1): S47-S51, ISSN: 1573-7446

 

REINA R., BERTOLOTTI L., DEI GIUDICI S., PUGGIONI G., PONTI N., PROFITI M., PATTA C., ROSATI S. (2010). Small Ruminant Lentivirus genotype E is widespread in Sarda Goat. VETERINARY MICROBIOLOGY, vol. 144; p. 24-31, ISSN: 0378-1135

 

CARROZZA ML, MAZZEI M, BANDECCHI P, FRAISIER C, PÉREZ M, SUZAN-MONTI M, DE ANDRÉS D, AMORENA B, ROSATI S., ANDRÉSDOTTIR V, LUJAN L, PEPIN M, BLACKLAWS B, TOLARI F, HARKISS GD. (2010). Development and comparison of strain specific gag and pol real-time PCR assays for the detection of Visna/maedi virus. JOURNAL OF VIROLOGICAL METHODS, vol. 165; p. 161-167, ISSN: 0166-0934

M. Juganaru, R. Reina, L. Bertolotti, M.C. Stella, M. Profiti, M. Armentano, E. Bollo,

B. Amorena, S. Rosati. (2011). In vitro properties of small ruminant lentivirus genotype E. VIROLOGY, vol. 409; in press. ISSN: 0042-6822

 

R. Reina, M. Juganaru, M. Profiti, P. Cascio, F. Cerruti, L. Bertolotti, D. De Meneghi, B. Amorena, S. Rosati. (2011). Immunological parameters in goats experimentally infected with SRLV genotype E, strain Roccaverano. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, in press, ISSN: 0165-2427

 

Bertolotti L, Mazzei M, Puggioni G, Carrozza ML, Dei Giudici S, Muz D, Juganaru M, Patta C, Tolari F, Rosati S. (2011). Characterization of new small ruminant lentivirus subtype B3 suggests animal trade within the Mediterranean Basin. J Gen Virol. 92:1923-9.

Tramuta C, Lacerenza D, Zoppi S, Goria M, Dondo A, Ferroglio E, Nebbia P, Rosati S. (2011) Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. J Vet Diagn Invest. 23 (4): 657-64.

Ramírez H, Reina R, Bertolotti L, Cenoz A, Hernández MM, San Román B, Glaria I, de Andrés X, Crespo H, Jáuregui P, Benavides J, Polledo L, Pérez V, García-Marín JF, Rosati S, Amorena B, de Andrés D. (2012)  Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep. BMC Vet Res. 26; 8:8.

 

 


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Rosati Sergio
Scienze di Base e Biotecnologie Veterinarie
Nebbia Carlo
Facoltà di Medicina Veterinaria, Dipartimento di Morfofisiologia Veterinaria
Mannelli Alessandro
Scienze di Base e Biotecnologie Veterinarie
Nebbia Carlo
Facoltà di Medicina Veterinaria, Dipartimento di Morfofisiologia Veterinaria
Nebbia Patrizia
Scienze cliniche Veterinarie
Farca
Facoltà di Medicina Veterinaria, Dipartimento di Morfofisiologia Veterinaria

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10