Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Defilippi Paola Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Defilippi Paola
Qualifica PO
Facoltà
Medicina e Chirurgia
Dipartimento Biotecnologie Molecolari e Scienze per la Salute
Settore Scientifico Disciplinare BIO13
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PERSONALE STRUTTURATO

Cognome e Nome
CRAVERO TIZIANA
Qualifica Tecnician
Dipartimento
Genetica, Biologia e Biochimica
Ente di appartenenza Università di Torino
Cognome e Nome TURCO EMILIA
Qualifica
PA
Dipartimento
Genetica, Biologia e Biochimica
Ente di appartenenza Università di Torino

PERSONALE NON STRUTTURATO

Cognome e Nome Grasso Silvia
Qualifica Post Doc
Dipartimento Biotecnologie Molecolari e Scienze per la Salute
Ente di appartenenza Università di Torino
Cognome e Nome Chapelle Jennifer
Qualifica PhD
Dipartimento Biotecnologie Molecolari e Scienze per la Salute
Ente di appartenenza Università di torino
Cognome e Nome Alfieri Annalisa
Qualifica PhD
Dipartimento Biotecnologie Molecolari e Scienze per la Salute
Ente di appartenenza Università di Torino
Cognome e Nome Salemme Vincenzo
Qualifica PhD
Dipartimento Biotecnologie Molecolari e Scienze per la Salute
Ente di appartenenza Università di Torino

LINEE DI RICERCA

Signalling platforms in normal and tranformed cells: molecular and functional characterisation and translational strategies.

The main interest of our lab is to investigate how cell signalling originating from integrin-dependent adhesion  and growth factor receptor co-ordinately regulate convergent pathways that control cell growth and migration in normal and transformed cells. Our current research is focused on the following points:
1.  the cross-talk between beta1 integrin and the EGF receptor
2.  the role of the adaptor protein p130Cas in development and tumorigenesis
3.  the role of the adaptor protein p140Cap in neurons and cancer cells.

Description

a) Signaling platforms in tumor transformation

A pressing clinical issue in cancer is the differentiation of cancer patients with indolent or aggressive disease, as well as those that would benefit from specific therapies. A selection of crucial players in the early onset of tumor metastasis, as well as homing of metastatic cells to target organs were discovered and validated at the preclinical level within the former FP7 program, MetaFight. As a coordinator of this project, our major aim is to work in an inter-disciplinary partnership on converting mechanistic insight relating to the machinery of tumor metastasis into novel diagnostic tools for better cancer patient management.

Since many years, we work on the integration of the signal between integrins and tyrosine kinase receptors, such as EGFR orHER2, in tumor progression. We also identified the adapter p140Cap as 'tumor suppressor' in breast cancer cells. Recently, we focused on the molecular mechanisms of action of p140Cap in HER2 + breast cancer, in the context of HER2amplicon on the long arm of chromosome 17. The problem is dealt with in vitro cellular models and in vivo animal models of tumorigenesis, such as the preclinical mouse model of breast cancer MMTV-NeuT, a MMTV-p140Cap model generated by us, to over express p140Cap in the mammary gland, p140Cap total KO and conditional floxed mice.

Moreover, in collaboration with Pathologists, we wish to perform:

1.   Evaluation of the prognostic potential of individual metastasis-related candidate biomarkers, like p140Cap, using retrospective cohorts of human tumor tissues

2.   Combinatorial assessment of individual candidate biomarkers towards definition of optimal prognostic set

3.   Exploration of the predictive utility of individual metastasis-related factors via the use of in vitro and in vivo modelling approaches.

b) Role of p140Cap family proteins in synaptic plasticity.

Synaptic plasticity regulates multiple functions, such as memory and behaviour. In turn, synaptic plasticity is controlled by complex mechanisms that include synaptic channels and receptor signalling. Our data indicate that the adapter protein p140Cap is highly expressed in neurons, with an enrichment in synaptic structures. Moreover we previously demonstrated that p140Cap knock out mice present cognitive deficits, impaired LTP and LTD and immature filopodia-like dendritic spines, indicating that p140Cap may be relevant for synaptic plasticity. However how p140Cap can regulate molecular events in dendritic spines is still unknown. The p140Cap protein family also includes its paralog, named Skt in mice and KIAA1217 in humans. Thanks to the generation of total and conditional KO mice for the genes encoding for these proteins, we want to investigate the role of these adapter sin synaptic plasticity. We will use morphological and biochemical assays, as well as electrophysiological and behavioural tests.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Production and characterisation of polyclonal and monoclonal antibodies
  • Cloning and expression of proteins in eukaryotic cells
  • Production of recombinant proteins in bacteria
  • Analysis of post-translational modifications (phosphorylation) by mass spectrometry
  • Analysis of signal transduction
  • Preparation of homologous recombination constructs in bacteria
  • Analysis of the development and differentiation of mice mammary gland

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Fluorescence microscopy and time lapse
Dipartimento di Biotecnologie Molecolari e Scienze per la salute
Di Cunto Ferdinando
FACS
HUGEF

PUBBLICAZIONI

http://www.ncbi.nlm.nih.gov/pubmed/?term=defilippi+p

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
DEFILIPPI Paola
Biotecnologie molecolari
Fiorella Altruda
Torino
TURCO Emilia
Biotecnologie molecolari
Fiorella Altruda
Torino

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10