Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Altruda Fiorella Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Altruda Fiorella
Qualifica PO Full Professor
Facoltà
Medical School
Dipartimento
Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Settore Scientifico Disciplinare BIO/13
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PERSONALE STRUTTURATO

Cognome e Nome
Emanuela Tolosano
Qualifica PA
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza
University of Turin
Cognome e Nome
Fiorella Altruda
Qualifica
PO
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza
University of Turin
Cognome e Nome Massimo Santoro
Qualifica
PA
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza
University of Turin

PERSONALE NON STRUTTURATO

Cognome e Nome Elvira Famulari
Qualifica PhD student
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza
University of Turin
Cognome e Nome Fagoonee Sharmila
Qualifica
Ricercatore CNR
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza
University of Turin
Cognome e Nome Chiabrando Deborah
Qualifica Assegnista
Dipartimento Dept. of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center
Ente di appartenenza University of Turin

LINEE DI RICERCA

Stem cells and regenerative medicine research is particularly active in the lab and regards mainly the use of stem cells for the cure of liver diseases. We have previously demonstrated that functional hepatocytes can be generated from mouse germline cell-derived pluripotent stem cells and that these cells can engraft in and participate to liver regeneration in vivo. Our aim is to generate functional hepatocytes from human stem cells in vitro,  and research is currently focused on:

a) Generating functional hepatocytes from human stem cells (human liver stem cells);

b) Correcting gene deficiency, responsible for liver metabolic diseases, with stem cells as platform for gene delivery or with microvesicles for delivery of therapeutic agents;

c) Studying the long term engraftment of stem cell-derived hepatocytes in the liver in mice models of liver diseases.

Another research area regards studying the role of an RNA-binding protein, ESRP1 in neoplastic transformation.  We came across this gene in a large-scale cDNA microarray analysis on mouse embryonic stem cells aiming at finding novel genes involved in stemness- and pluripotency- maintenance. ESRP1 is  responsible for epithelial differentiation and we are now studying:

a) The role of ESRP1, by modulating its expression, in the neoplastic transformation of epithelial cells

b) Tumor growth (primary and metastatic) in vivo in immunocompromised animals


TECNOLOGIE IN POSSESSO DELL'U. O.

  • Transgenic mice generation
  • KO mice
  • Knock-in mice
  • Gene expression analysis by real-time PCR

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
3 Inverted Microscopy stations with Nomarski
Molecular Biotechnology Center
Fiorella Altruda e Emilio Hirsch
Real time PCR
Molecular Biotechnology Center
Sharmila Fagoonee

PUBBLICAZIONI

- Ognibene M, Barbieri O, Vanni C, Mastracci L, Astigiano S, Emionite L, Salani B, Fedele M, Resaz R, Tenca C, Fais F, Sabatini F, De Santanna A, Altruda F, Varesio L, Hirsch E, Eva A. High frequency of development of B cell lymphoproliferation and diffuse large B cell lymphoma in Dbl knock-in mice. J Mol Med. 2011 May;89(5):493-504. Epub 2011 Jan 11

- Morello N, Bianchi FT, Marmiroli P, Tonoli E, Rodriguez Menendez V, Silengo L, Cavaletti G, Vercelli A, Altruda F, Tolosano E. A Role for Hemopexin in Oligodendrocyte Differentiation and Myelin Formation PloS ONE, 2011;6(5):e20173

- Fagoonee S, Pellicano R, Silengo L, Altruda F. Potential applications of germline cell-derived pluripotent stem cells in organ regeneration. Organogenesis. 2011 Apr 1;7(2):116-22. doi: 10.4161/org.7.2.16284. Epub 2011 Apr 1.

- Fiorito V, Geninatti Crich S, Silengo L, Altruda F, Aime S, Tolosano E. Assessment of iron absorption in mice by ICP-MS measurements of (57)Fe levels. Eur J Nutr. 2012 Oct;51(7):783-9. Epub 2011 Oct 19.

- Robin M. Hobbs, Sharmila Fagoonee, Antonella Papa, Kaitlyn Webster, Fiorella Altruda, Ryuichi Nishinakamura, Li Chai, Pier Paolo Pandolfi Functional Antagonism between Sall4 and Plzf Defines Germline Progenitors. Cell Stem Cell, Volume 10, Issue 3, 284-298, 2 March 2012

- Chiabrando D, Marro S, Mercurio S, Giorgi C, Petrillo S, Vinchi F, Fiorito V, Fagoonee S, Camporeale A, Turco E, Merlo GR, Silengo L, Altruda F, Pinton P, Tolosano E. The mitochondrial heme exporter FLVCR1b mediates erythroid differentiation. J Clin Invest. 2012 Dec 3;122(12):4569-79. doi: 10.1172/JCI62422. Epub 2012 Nov 26

- Chiabrando D, Fiorito V, Marro S, Silengo L, Altruda F, Tolosano E. Cell-specific regulation of Ferroportin transcription following experimentally-induced acute anemia in mice. Blood Cells Mol Dis. 2013 Jan;50(1):25-30. doi: 10.1016/j.bcmd.2012.08.002. Epub 2012 Aug 24

- Vinchi F, De Franceschi L, Ghigo A, Townes T, Cimino J, Silengo L, Hirsch E, Altruda F, Tolosano E. Hemopexin Therapy Improves Cardiovascular Function by Preventing Heme-Induced Endothelial Toxicity in Mouse Models of Hemolytic Diseases. Circulation. 2013 Mar 26;127(12):1317-29. doi: 10.1161/CIRCULATIONAHA.112.130179. Epub 2013 Feb 27.

- Fiorito V, Geninatti Crich S, Silengo L, Aime S, Altruda F, Tolosano E. Lack of Plasma Protein Hemopexin Results in Increased Duodenal Iron Uptake. PLoS One. 2013 Jun 27;8(6):e68146.

- Sharmila Fagoonee, Claudia Bearzi, Ferdinando Di Cunto, John G.Clohessy, Roberto Rizzi, Marcus O. Reschke,  Emanuela Tolosano, Paolo Provero , Pier Paolo Pandolfi, Lorenzo Silengo and Fiorella Altruda. The RNA binding protein ESRP1 fine-tunes the expression of pluripotency-related factors in mouse embryonic stem cells. PLoS One, 2013 Aug 27;8 (8):e72300.DOI: 10.1371.

- Rolla S, Ingoglia G, Bardina V, Silengo L, Altruda F, Novelli F, Tolosano E. Acute-Phase Protein Hemopexin Is a Negative Regulator of Th17 Response and Experimental Autoimmune Encephalomyelitis Development. J Immunol. 2013 Dec 1;191(11):5451-9. doi: 10.4049/jimmunol.1203076. Epub 2013 Oct 23.

- Letizia De Chiara, Sharmila Fagoonee, Andrea Ranghino, Stefania Bruno, Giovanni Camussi, Emanuela Tolosano, Lorenzo Silengo and Fiorella Altruda. Renal cells from germline stem cells protect against kidney injury. J Am Soc Nephrol. 2014 Feb;25(2):316-28. doi: 10.1681/ASN.2013040367. Epub 2013 Oct 17.

- Maccarinelli F, Gammella E, Asperti M, Regoni M, Biasiotto G, Turco E, Altruda F, Lonardi S, Cornaghi L, Donetti E, Recalcati S, Poli M, Finazzi D, Arosio P, Cairo G. Mice lacking mitochondrial ferritin are more sensitive to doxorubicin-mediated cardiotoxicity. J Mol Med (Berl). 2014 Aug;92(8):859-69. doi: 10.1007/s00109-014-1147-0. Epub 2014 Apr 13.

- Fiorito V, Neri F, Pala V, Silengo L, Oliviero S, Altruda F, Tolosano E. Hypoxia controls Flvcr1 gene expression in Caco2 cells through HIF2α and ETS1. Biochim Biophys Acta. 2014 Apr;1839(4):259-64. doi: 10.1016/j.bbagrm.2014.02.010. Epub 2014 Feb 24. PubMed PMID: 24576667.

- Vinchi F, Ingoglia G, Chiabrando D, Mercurio S, Turco E, Silengo L, Altruda F, Tolosano E. Heme Exporter FLVCR1a Regulates Heme Synthesis and Degradation and Controls Activity of Cytochromes P450. Gastroenterology. 2014 May;146(5):1325-38. doi: 10.1053/j.gastro.2014.01.053. Epub 2014 Jan 31.

- Rusconi F, Paganini L, Braida D, Ponzoni L, Toffolo E, Maroli A, Landsberger N, Bedogni F, Turco E, Pattini L, Altruda F, De Biasi S, Sala M, Battaglioli E. LSD1 Neurospecific Alternative Splicing Controls Neuronal Excitability in Mouse Models of Epilepsy. Cereb Cortex. 2014 Apr 15. [Epub ahead of print]

- Mugoni V, Camporeale A, Santoro MMAnalysis of oxidative stress in zebrafish embryos. J Vis Exp. 2014 Jul 7;(89). doi: 10.3791/51328.

- Santoro MMZebrafish as a model to explore cell metabolism. Trends Endocrinol Metab. 2014 Oct;25(10):546-54. doi: 10.1016/j.tem.2014.06.003. Epub 2014 Jul 2. Review. Trends Endocrinol Metab

- Santoro MMAntiangiogenic cancer drug using the zebrafish model. Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):1846-53.

- Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, Santoro MM, Childs SJ. An α-smooth muscle actin (acta2/αsma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells. PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014.

- Mugoni V, Postel R, Catanzaro V, De Luca E, Turco E, Digilio G, Silengo L, Murphy MP, Medana C, Stainier DY, Bakkers J, Santoro MM. Ubiad1 is an antioxidant enzyme that regulates eNOS activity by CoQ10 synthesis. Cell. 2013 Jan 31;152(3):504-18. doi: 10.1016/j.cell.2013.01.013.

- Garaffo G, Conte D, Provero P, Tomaiuolo D, Luo Z, Pinciroli P, Peano C, D'Atri I, Gitton Y, Etzion T, Gothilf Y, Gays D, Santoro MM, Merlo GR. The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system. Mol Cell Neurosci. 2015 Apr 30;68:103-119. doi: 10.1016/j.mcn.2015.04.007.

- Gays D, Santoro MM. The admiR-able advances in cardiovascular biology through the zebrafish model system. Cell Mol Life Sci. 2013 Jul;70(14):2489-503. doi: 10.1007/s00018-012-1181-4. Epub 2012 Oct 16. Review.

 


DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Fiorella Altruda
Medicina Molecolare curriculum Bitecnologie Molecolari
Fiorella Altruda
Torino
Emanuela Tolosano
Scienze Biomediche e Oncologia Emilio Hirsch Torino

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4
si
CNB5
si
CNB6
si
CNB7
si
CNB8
si
CNB9 si
CNB10 si