Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Bozzoni Irene Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome
Bozzoni Irene
Qualifica Full Professor
Facoltà
La Sapienza
Dipartimento
Genetics and Molecular Biology
Settore Scientifico Disciplinare Bio-11
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PERSONALE STRUTTURATO

Cognome e Nome
Arceci Massimo
Qualifica
Researcher
Dipartimento
IBPM
Ente di appartenenza
CNR - Rome
Cognome e Nome Caffarelli Elisa
Qualifica
Researcher
Dipartimento
IBPM
Ente di appartenenza
CNR - Rome
Cognome e Nome
Carlo Presutti
Qualifica Associate Professor
Dipartimento Dept. of Genetics and Molecular Biology
Ente di appartenenza
University "La Sapienza"
Cognome e Nome
Fatica Alessandro
Qualifica
Researcher
Dipartimento Dept. of Genetics and Molecular Biology
Ente di appartenenza
University "La Sapienza"
Cognome e Nome Fragapane Paola
Qualifica
Researcher
Dipartimento
IBPM
Ente di appartenenza
CNR - Rome
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

PERSONALE NON STRUTTURATO

Cognome e Nome Ballarino Monica
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome De Angelis Fernanda
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Denti Michela
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Gioia Ubaldo
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Laneve Pietro
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Morlando Mariangela
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Rosa Alessandro
Qualifica
Post-doc
Dipartimento
Ente di appartenenza
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

RNA factory

The knowledge of the complexity of the machinery that participates in gene expression has significantly changed with the finding that a tight coupling exists between transcriptional and post-transcriptional processes. The carboxy-terminal domain of the polII large subunit drives on the nascent transcripts several factors required for post-transcriptional events: capping, splicing, polyadenylation. We are interested in studying the RNA-protein interactions that occur during the early phases of transcription and commit the primary transcript to its specific maturation fate. In particular, we are studying the biosynthesis of the complex class of small non coding RNAs including miRNAs, snoRNAs and snRNAs

Biosynthesis and function of miRNAs

In the last years a new network of regulatory circuits operating at the post-transcriptional level has been disclosed with the discovery of a complex class of non-coding RNAs of microscopic size (miRNAs). These molecules control gene reprogramming occurring during development and cell differentiation by regulating translation and stability of specific target mRNAs. We are interested in the following aspects of miRNA research: i) identify the regulatory regions required for their transcription and 3 end formation; ii) understand the mechanisms of translational and stability control; iii) study their role in the control of cell differentiation with specific interest in hematopoietic and neuronal differentiation.

Therapeutic RNAs

Antisense, ribozymes, aptamers and, more recently, siRNAs are modified activities of natural RNAs that can be utilized to control gene expression in a sequence-specific way. In the last years we have exploited some of these RNA activities for the gene therapy of different genetic diseases. One succeful application has been the use of antisense molecules for the gene therapy of the Duchenne Muscular Dystrophy. Recent work in the mdx dystrophic mouse has shown that AAV recombinant viruses carrying antisense-expression cassettes are very effective in systemic in vivo delivery of therapeutic sequences to affected muscles and in restoring both dystrophin synthesis and muscular functionality. In the future we intend to extend the use of other small non coding RNAs (siRNAs and miRNAs) to the therapy of different gene disorders.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Techniques of RNA analysis including miRNA identification and cloning
  • RNAi and antisense methodology to interfere with gene expression
  • Vectors for sncRNA expression
  • ChIP analysis
  • Protein tagging and purification of native complexes

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Phosphoimager
Department of Genetics and Molecular Biology

Real Time PCR
Department of Genetics and Molecular Biology

Atomic force microscope
Department of Genetics and Molecular Biology

P2 laboratory
Department of Genetics and Molecular Biology

Apparatus for microinjection
Department of Genetics and Molecular Biology

PUBBLICAZIONI

1. Fatica, A., Morlando M. and Bozzoni I. (2000) Yeast snoRNA accumulation relies on a cleavage-dependent/polyadenylation-independent 3’ processing apparatus. EMBO J., 19, 6218-6229.
2. Bousquet-Antonelli C, Presutti C, Tollervey D (2000) .Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell.102:765-75.
3. Giorgi C, Fatica A, Nagel R, Bozzoni I. (2001) Release of U18 snoRNA from its host intron requires interaction of Nop1p with the Rnt1p endonuclease. EMBO J. 20:6856-6865.
4. Galardi S, Fatica A, Bachi A, Scaloni A, Presutti C and Bozzoni I. (2002) Purified box C/D snoRNPs are able to reproduce site-specific 2’-O-methylation of target RNA Mol. Cell. Biol. 22:6663-8.
5. De Angelis F., Sthandier O. , Berarducci B., Toso S., Galluzzi G., Ricci E., Cossu G. and Bozzoni I. (2002) Chimeric snRNA molecules carrying antisense sequences against the splice junctions of exon 51 of the dystrophin pre-mRNA induce exon skipping and restoration of a corrected phenotype in 48-50 DMD cells, Proc. Natl. Acad. Sci. USA, 99:9456-61.
6. Denti M.A., Rosa A., Sthandier O., De Angelis G. and Bozzoni I. (2004) A novel vector for siRNA expression in mammalian cells that relies on polII-dependent transcription, Mol Ther. 10, 191-199.
7. Morlando M., Ballarino M., Greco P., Caffarelli E., Dichtl B. and Bozzoni I. (2004) Coupling between snoRNP assembly and the transcriptional/3’ processing apparatus controls box C/D snoRNA biosynthesis in yeast, EMBO J., 23:2392-2401.
8. Ballarino M., Morlando M, Pagano F., Fatica A. and Bozzoni I. (2005) The co- transcriptional assembly of snoRNPs controls the biosynthesis of H/ACA snoRNAs in S.cerevisiae- Mol. Cell. Biol., 25: 5396-403.
9. Fazi F., Rosa A., Fatica A., De Marchis M. L., Gelmetti V., Nervi C. and Bozzoni I. (2005) A mini-circuitry comprising microRNA-223 and transcription factors NFI-A and C/EBP regulates human granulopoiesis. Cell, 123: 819-831.
10. Denti M.A., Rosa A., D’Antona G., Sthandier O., De Angelis F.G., Nicoletti C., Allocca M., Pansarasa O., Parente V., Musarò A., Auricchio A., Bottinelli R. and Bozzoni I. (2006) Body-wide gene therapy of Duchenne Muscular Dystrophy in the mdx mouse model. Proc. Natl. Acad. Sci. USA, in press.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
I. Bozzoni
PhD Programme in Genetics and Molecular Biology
I. Bozzoni

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10