Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Valeria Dall'Asta Stampa
RESPONSABILE DELLA U. O.

Valeria Dall'Asta
Cognome e Nome Valeria Dall'Asta
Qualifica Professore Ordinario
Facoltà Università di Parma
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Settore Scientifico Disciplinare MED04
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PERSONALE STRUTTURATO

Cognome e Nome
Bussolati Ovidio
Qualifica
PA
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma
Cognome e Nome
Rotoli Bianca Maria
Qualifica
RU
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma
Cognome e Nome Sala Roberto
Qualifica
RU
Dipartimento
Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma
Cognome e Nome
Visigalli Rossana
Qualifica
Tecnico
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma

PERSONALE NON STRUTTURATO

Cognome e Nome
Barilli Amelia
Qualifica Assegnista
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma
Cognome e Nome
Bianchi Massimiliano
Qualifica Assegnista
Dipartimento
Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma
Cognome e Nome Tardito Saverio
Qualifica Dottorando
Dipartimento Dipartimento di Scienze Biomediche Biotecnologiche e Traslazionali, SBiBiT, Unità di Patologia Generale
Ente di appartenenza Università di Parma

LINEE DI RICERCA

-Endothelial cell damage in vitro model of Ischemic-reperfusion injury: role of arginine and nitric oxide production. The study aim to investigate how changes in arginine availability affect viability and activation of endothelial cells under conditions of hypoxia-reoxygenation. The experimental models consist in endothelial cells exposed to hypoxic-reoxigenation stress, treated with pro-inflammatory cytokines, or co-cultured with monocytes-macrophages. To approach in vivo conditions and stress endothelial damage, arginine-free media or media containing low concentrations of the cationic amino acid will be employed. Under these experimental conditions, toxic effects exerted by hypoxia-reoxygenation and/or treatment with pro-inflammatory cytokines will be investigated. The selected endothelial models consist of human endothelial cells from umbilical veins (HUVECs), as well as of adult endothelial strains, obtained from saphenous veins (HSVECs). For co-cultures of endothelial cells and monocytes/macrophages, monocytes freshly isolated from buffy coat or THP-1 (human monocytes cell line) will be employed.
-Pathogenesis of pulmonary alveolar proteinosis in Lisinuria protein intolerance (LPI): application of in vitro cellular models. The study of the pathogenesis of the LPI extra-renal alterations would require a great availability of pathologic cells from patients, a requirement hard to satisfy. Aim of this project is overcome this problem through the production of in vitro cell models with the same characteristic of the pathologic cells in vivo. The latter possibility is strengthened by the evidence, recently obtained by our group, that both monocytic and airway respiratory human cells (CALU-3 cell line) express system y+L and, in particular, the SLC7A7/y+LAT1 isoform mutated in LPI. These cell types may, therefore, express the LPI defect and hence, be involved in the pathogenesis of pulmonary alveolar proteinosis (PAP), a rare respiratory disease in which lipoproteinaceous material accumulates within alveoli, that is often the immediate cause of death in LPI.. Therefore, the objective pursued by this Unit will consist in the production of LPI cell models through small interference RNA (siRNA) of SLC7A7 expression.
-The role played by inflammation on the differentiation process of the stem cells. The in vivo situation after an ischemic event is characterized by a strong inflammatory response, with a number of cytokines and chemokines acting trough both paracrine and autocrine way to stimulate the activity of resident or immigrant cells. To achieve our goal we will establish culture conditions able to amplify CD34+ and CD133+ bone marrow-mobilized and blood cord stem cell unsuitable for therapeutic use, maintaining their stem cell markers; stem cells will be isolated by density gradient and further purified with specific antibodies by an immunomagnetic commercially available kit; stem progenitor cells from mouse bone marrow will be purified and identified, too. Thereafter, we will study in vitro the effects of the plasmatic mediators of inflammation on the capability of mature endothelial cells and fibroblasts in inducing the differentiation of previously characterized stem cells. We will compare the effects of the endothelial cells growth factors VEGF and FGF on the CD34+ and CD133+ differentiation process with the ones possessed by the cytokine induced differentiated cells. The cocultures between stem cells and differentiated cells, i.e. fibroblasts, endothelial cells, mouse cardiac myocytes stimulated for various time with well-known mediators of the inflammation will be used to such aim.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Tissue dissociation and cell cultures
  • Fluorescence of living cells and laser scanning confocal microscopy
  • Analysis of ions and aminoacids cell transport
  • Aminoacids determination in cells and in biological fluids
  • Signalling Transduction pathways analysis
  • Cell transfection/expression of tagged proteins
  • PCR, RT-PCR and DNA cloning
  • Northern, Southern and Western blot analysis
  • 1D and 2D-Electrophoresis
  • Densitometric analysis

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Beckmann -Ultracentifuge–L60
Dipartimento SBiBiT
Dr. Visigalli Rossana
Corbett- rotor gene 3000
Dipartimento SBiBiT
Dr. Sala Roberto
Pharmacia Biotec Biochrom 20 -
Dipartimento SBiBiT
Prof. Dall’Asta Valeria
Zeiss- LSM 510 META Confocal microscope
Dipartimento SBiBiT
Prof. Gatti Rita

PUBBLICAZIONI

1. Bevilacqua E, Bussolati O, Dall'Asta V, Gaccioli F, Sala R, Gazzola GC, Franchi-Gazzola R. SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress. FEBS Lett. 2005 Jun 20;579(16):3376-80.
2. Rotoli BM, Bussolati O, Sala R, Gazzola GC, Dall'Asta V. The transport of cationic amino acids in human airway cells: expression of system y+L activity and transepithelial delivery of NOS inhibitors. FASEB J. 2005 May;19(7):810-2.
3. Rotoli BM, Uggeri J, Dall'Asta V, Visigalli R, Barilli A, Gatti R, Orlandini G, Gazzola GC, Bussolati O. Inhibition of glutamine synthetase triggers apoptosis in asparaginase-resistant cells. Cell Physiol Biochem. 2005;15(6):281-92.
4. Rotoli BM, Bussolati O, Sala R, Barilli A, Talarico E, Gazzola GC, Dall'Asta V. INFgamma stimulates arginine transport through system y+L in human monocytes. FEBS Lett. 2004 Jul 30;571(1-3):177-81.
5. Rotoli BM, Orlandini G, Guizzardi S, Uggeri J, Dall'Asta V, Gazzola GC, Bussolati O, Gatti R. Ethanol increases the paracellular permeability of monolayers of CAPAN-1 pancreatic duct cells. J Mol Histol. 2004 May;35(4):355-62.
6. Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Bataille R, Almici C, Caramatti C, Mangoni L, Rizzoli V. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Blood. 2003 Jul 15;102(2):638-45.
7. Rotoli BM, Orlandini G, Gatti R, Dall'Asta V, Gazzola GC, Bussolati O. Employment of confocal microscopy for the dynamic visualization of domes in intact epithelial cell cultures.Cells Tissues Organs. 2002;170(4):237-45.
8. Alamanni F, Parolari A, Visigalli R, Bussolati O, Rubini P, Sala R, Bonati L, Gazzola GC, Biglioli P, Dall'Asta V. Endothelial cell injury induced by preservation solutions: a confocal microscopy study. Ann Thorac Surg. 2002 May;73(5):1606-14.
9. Sala R, Rotoli BM, Colla E, Visigalli R, Parolari A, Bussolati O, Gazzola GC, Dall'Asta V. Two-way arginine transport in human endothelial cells: TNF-alpha stimulation is restricted to system y(+). Am J Physiol Cell Physiol. 2002 Jan;282(1):C134-43.
10. Franchi-Gazzola R, Visigalli R, Dall'Asta V, Sala R, Woo SK, Kwon HM, Gazzola GC, Bussolati O. Amino acid depletion activates TonEBP and sodium-coupled inositol transport.Am J Physiol Cell Physiol. 2001 Jun;280(6):C1465-74.

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede
Dall'Asta Valeria
Biologia e Patologia molecolare
Prof. Valeria Dall'Asta
Parma
Bussolati Ovidio
Biologia e Patologia molecolare
Prof. Valeria Dall'Asta
Parma
Rotoli Bianca Maria
Biologia e Patologia molecolare
Prof. Valeria Dall'Asta
Parma
Sala Roberto
Biologia e Patologia molecolare
Prof. Valeria dall'Asta
Parma

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10