Direzione e uffici C.I.B.

Direzione CIB:
Prof. Claudio Schneider
Email: claudio.schneider@Lncib.it

Segreteria CIB:
Prof. Roberto Gambari
Email: roberto.gambari@unife.it

SEGRETERIA ORGANIZZATIVA:
Elisabetta Lambertini
Tel: 0532/974451
Fax: 0532/974484
E-mail: lmblbt@unife.it

AMMINISTRAZIONE:
Vanessa Florit
Area di Ricerca
Padriciano, 99 - 34012 Trieste
Tel: 040/398979
Fax: 040/398990
E-mail: cib@lncib.it

Posta certificata C.I.B.:
cib@poste-certificate.it

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Cimino Filiberto Stampa
RESPONSABILE DELLA U. O.

Cognome e Nome Cimino Filiberto
Qualifica PO
Facoltà Medicina e Chirurgia Università di Napoli Federico
Dipartimento Biochimica e Biotecnologie Mediche, Settore scientifico disciplinare
Settore Scientifico Disciplinare BIO/10
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PERSONALE STRUTTURATO

Cognome e Nome
Ammendola Rosario
Qualifica
PO
Dipartimento Dipartimento di Scienze per la Salute
Ente di appartenenza Università del Molise
Cognome e Nome Bevilacqua M.Assunta
Qualifica
PA
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza Università di Napoli Federico II
Cognome e Nome Di Marzo Domenico
Qualifica
Dottoranda in Biochimica e Biologia Cellulare e Molecolare
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza Università di Napoli Federico II

PERSONALE NON STRUTTURATO

Cognome e Nome Calise Serena
Qualifica
Dottoranda in Biochimica e Biologia Cellulare e Molecolare
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza
Università di Napoli Federico II
Cognome e Nome
Iaccio Annalisa
Qualifica Specializzanda in Biochimica Clinica
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza
Università di Napoli Federico II
Cognome e Nome
Iovine Barbara
Qualifica Specializzanda in Biochimica Clinica
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza
Università di Napoli Federico II
Cognome e Nome
Montesano Gesualdi Nicola
Qualifica
Dottorando in Biochimica e Biologia Cellulare e Molecolare
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza
Università di Napoli Federico II
Cognome e Nome
Vergara Paola
Qualifica
Dottoranda in Biochimica e Biologia Cellulare e Molecolare
Dipartimento Dipartimento di Biochimica e Biotecnologie Mediche
Ente di appartenenza
Università di Napoli Federico II
Cognome e Nome
Qualifica
Dipartimento
Ente di appartenenza

LINEE DI RICERCA

A) Redox regulation of the expression of genes involved in the induction of a senescent phenotype (Prof. Bevilacqua) Previous studies allowed us to identify molecular markers selective for aging and/or cellular senescence, respectively: among them, fibromodulin (FM) whose mRNA levels are induced in growing fibroblasts, whereas are highly decreased in senescent cells. We have recently demonstrated in growing fibroblasts that FM expression is regulated by DDB1, a protein involved in the repair of UV-damaged DNA. Experiments are in progress to study the regulation of FM expression upon UV treatment, as well as the pathway of repair induced in these experimental conditions.
B) Molecular mechanisms responsible for the induction of oxidative stress- and apoptosis-resistant phenotypes ( Prof. Faraonio) NIH3T3 clones resistant to a mild oxidative stress have been selected, and several genes, whose expression is regulated in resistant cells, have been identified. The main interest of this group is to study the regulation of the expression of x-CT, a cystine-glutamate transporter, whose mRNA levels are highly induced in all the analyzed NIH resistant clones. Experiments are in progress to investigate the role of nrf2, a stress-related transcription factor involved in the regulation of the expression of x-CT gene.
C) Structural and functional characterization of genes involved in the regulation of adaptive response to oxidative stress and chemoresistance (Prof. Esposito) Saos-2 human osteosarcoma cells adapted to a mild oxidative stress induced by diethylmaleate (DEM) have been selected. The expression of two genes (AROS-29, an EST coding for a transmembrane protein of unknown function, and TRAP-1, a mitochondrial heat shock) was similarly induced both in DEM-adapted, as well as in cisplatin and/or 5-FU resistant cells: experiments are in progress to study the correlations between adaptation to oxidative stress and chemoresistance.
D) Molecular mechanisms involved in the activation of NADPH oxidase by formyl peptides in non-phagocytic cells (Prof. Ammendola) The main interst of this project is to study the mechanisms of NADPH oxidase activation by formyl peptides in eukaryotic cells, as well as the identification of natural ligands for FPRL-1 receptor, recently cloned by our group in human fibroblasts.

TECNOLOGIE IN POSSESSO DELL'U. O.

  • Biochemical
  • cellular and molecular biology technologies for genomic and proteomic analysis

STRUMENTAZIONE

Denominazione
Struttura ove la strumentazione è allocata
Responsabile della strumentazione
Storm 840 imaging system
Dipartimento di Biochimica e Biotecnologie Mediche
Prof. Filiberto Cimino
, iCycler iQ Real Time PCR
Dipartimento di Biochimica e Biotecnologie Mediche
Prof. Filiberto Cimino
Ultracentrifuges
Dipartimento di Biochimica e Biotecnologie Mediche
Prof. Filiberto Cimino
Work Station for set up and analysis of fluorescent 2D gels (DIGE)
Dipartimento di Biochimica e Biotecnologie Mediche
Prof. Filiberto Cimino

PUBBLICAZIONI

1. Esposito F, Russo L, Russo T, Cimino F. Retinoblastoma protein dephosphorylation is an early event of cellular response to prooxidant conditions. FEBS Lett. 2000 Mar 24;470(2):211-5
2. Esposito F, Russo L, Chirico G, Ammendola R, Russo T, Cimino F. Regulation of p21waf1/cip1 expression by intracellular redox conditions. IUBMB Life. 2001 Jul;52(1-2):67-70.
3. Esposito F, Russo T, Cimino F. Generation of prooxidant conditions in intact cells to induce modifications of cell cycle regulatory proteins. Methods Enzymol. 2002;352:258-68.
4. Ammendola R, Ruocchio MR, Chirico G, Russo L, De Felice C, Esposito F, Russo T, Cimino F. Inhibition of NADH/NADPH oxidase affects signal transduction by growth factor receptors in normal fibroblasts. Arch Biochem Biophys. 2002 Jan 15;397(2):253-7.
5. Faraonio R, Pane F, Intrieri M, Russo T, Cimino F. In vitro acquired cellular senescence and aging-specific phenotype can be distinguished on the basis of specific mRNA expression. Cell Death Differ. 2002 Aug;9(8):862-46.
6. Esposito F, Chirico G, Montesano Gesualdi N, Posadas I, Ammendola R, Russo T, Cirino G, Cimino F. Protein kinase B activation by reactive oxygen species is independent of tyrosine kinase receptor phosphorylation and requires SRC activity. J Biol Chem. 2003 Jun 6;278(23):20828-34. Epub 2003 Apr 7.
7Ammendola R, Russo L, De Felice C, Esposito F, Russo T, Cimino F. Low-affinity receptor-mediated induction of superoxide by N-formyl-methionyl-leucyl-phenylalanine and WKYMVm in IMR90 human fibroblasts. Free Radic Biol Med. 2004 Jan 15;36(2):189-200
8. Esposito F, Ammendola R, Faraonio R, Russo T, Cimino F. Redox control of signal transduction, gene expression and cellular senescence. Neurochem Res. 2004 Mar;29(3):617-28
9. Bevilacqua MA, Iovine B, Zambrano N, D'Ambrosio C, Scaloni A, Russo T, Cimino F. Fibromodulin gene transcription is induced by ultraviolet irradiation, and its regulation is impaired in senescent human fibroblasts. J Biol Chem. 2005 Sep 9;280(36):31809-17. Epub 2005 Jul 6
10. R. Faraonio, P. Vergara, D. Di Marzo, M. Napolitano T. Russo, F. Cimino Transcription regulation in NIH3T3 cell clones resistant to diethylmaleate-induced oxidative stress and apoptosis, Antioxid & Redox Signal, 2006 in press

DOTTORATI DI RICERCA

Componente U.O. Dottorato di Ricerca Coordinatore Sede

CONGRESSI C.I.B.

Congressi Partecipazione
CNB4

CNB5

CNB6

CNB7

CNB8

CNB9
CNB10